A5013863101- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hypothalamic control of reproductive hormones
- Animal Genetics and Reproduction
- Neuropeptides and Animal Physiology
- Sperm and Testicular Function
- Aquaculture disease management and microbiota
- Meat and Animal Product Quality
- Fish Ecology and Management Studies
- Estrogen and related hormone effects
- Genetic and phenotypic traits in livestock
- Identification and Quantification in Food
- Plant Reproductive Biology
- Virus-based gene therapy research
- Hippo pathway signaling and YAP/TAZ
- Marine and fisheries research
- Neuroendocrine regulation and behavior
- Congenital heart defects research
- Animal Behavior and Reproduction
- CRISPR and Genetic Engineering
- Receptor Mechanisms and Signaling
- Animal Nutrition and Physiology
- Regulation of Appetite and Obesity
- Viral Infectious Diseases and Gene Expression in Insects
Consejo Superior de Investigaciones Científicas
2012-2024
Instituto de Acuicultura Torre de la Sal
2015-2024
Texas Tech University
2014-2018
Hospital Universitario La Paz
2013
Universidad Autónoma de Madrid
2013
National Academies of Sciences, Engineering, and Medicine
2011
Instituto de Investigaciones Agropecuarias
2011
University of Würzburg
1997-2003
This study, conducted in the brain of a perciform fish, European sea bass, aimed at raising antibodies against precursor kisspeptins order to map kiss systems and correlate expression kisspeptins, kiss1 kiss2, with that kisspeptin receptors (kiss-R1 kiss-R2). Specific could be raised preprokiss2, but not preoprokiss1. The data indicate kiss2 neurons are mainly located hypothalamus project widely subpallium pallium, preoptic region, thalamus, pretectal area, optic tectum, torus...
The endocrine pathways controlling vertebrate spermatogenesis are well established in mammals where the pituitary gonadotropins follicle-stimulating hormone (FSH) and luteinizing (LH) exclusively activate FSH receptor (FSHR) Sertoli cells LH/choriogonadotropin (LHCGR) Leydig cells, respectively. In some teleosts, however, it has been shown that Lh can cross-activate Fshra ortholog, coexpress Lhcgrba paralogs, thus mediating androgenic function of Fsh testis. Here, we investigated whether...
Different yields, biopotency, and in vivo pharmacokinetics are obtained for recombinant sea bass gonadoltropins depending on the production system DNA construct, but they show specific activation of their corresponding receptors. Gonadotropins (GTHs) glycoprotein hormones that play a major role regulation gonadal functions. Recently, we succeeded isolating native Fsh from pituitaries, to ensure availability bioactive GTHs no cross-contamination with other related glycoproteins, were produced...
Follicle-stimulating hormone (Fsh) is thought to act early in the process of spermatogenesis; however, its action fish has not yet been clearly established. In present work, we analyzed effects recombinant Fsh sea bass (Dicentrarchus labrax) spermatogenesis according two different approaches: direct injection single-chain (scFSH) and scFSH coding sequence. Both approaches were efficient increasing plasma at 7 15 days, respectively, after injection. The increment caused a significant increase...
Abstract Gonadotropin‐inhibitory hormone (GnIH) is a neuropeptide that suppresses reproduction in birds and mammals by inhibiting GnRH gonadotropin secretion. GnIH orthologs with C‐terminal LPXRFamide (LPXRFa) motif have been identified teleost fish. Although recent work also suggests its role fish reproduction, studies are scarce controversial, mainly focused on cyprinids. In this we cloned full‐length cDNA encoding an LPXRFa precursor the European sea bass, Dicentrarchus labrax . contrast...
Some teleost species, including European sea bass, harbor two different kisspeptin coding genes: kiss1 and kiss2. Both genes are expressed in the brain, but their differential roles central control of fish reproduction only beginning to be elucidated. In this study, we have examined effects intracerebroventricular injections highly active bass peptides Kiss1-15 Kiss2-12 on spermiating male bass. Physiological saline, Kiss1-15, or was injected into third ventricle. To establish gene...
Kisspeptins are key players in the neuroendocrine control of puberty and other reproductive processes mammals. Several studies have demonstrated that KISS/GPR54 system is expressed by gonadotrophs, but vitro assessing direct stimulatory effects kisspeptin on gonadotropin secretion pituitary provided conflicting results. In this study, we investigated whether directly influences function sea bass pituitary. First, highly active peptides Kiss1-15 Kiss2-12 were used to stimulate dispersed cells...
Pituitary gonadotropins, follicle-stimulating hormone (FSH), and luteinizing (LH) act via their cognate glycoprotein receptors (GpHRs), FSH receptor (FSHR), LH/choriogonadotropin (LHCGR) to regulate gonad physiology. Here, we show that the flatfish Senegalese sole (Solea senegalensis) expresses functional isoforms of fshr lhcgr, but genomic origin, ligand activation, tissue distribution transcripts are more complex than expected. By integrating molecular phylogeny GpHRs with syntenic loci...
Follicle-stimulating hormone (FSH) is a glycoprotein that plays key role in the regulation of gonadal functions vertebrates. The present study reports monitoring pituitary and plasma Fsh levels during sex differentiation oogenesis European sea bass (Dicentrarchus labrax) using homologous immunoassay an vitro bioassay. Both assays were used complementarily for first time fish species. High bioactivity found initial phases sexual differentiation. Plasma (quantity bioactivity) biological to...