A5014952697- Adenosine and Purinergic Signaling
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Neonatal Respiratory Health Research
- Neuroscience and Neural Engineering
- Neuroendocrine regulation and behavior
- Neuroscience of respiration and sleep
- Cardiac electrophysiology and arrhythmias
- Lipid Membrane Structure and Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Connexins and lens biology
- Digestive system and related health
- Pancreatic function and diabetes
- Nicotinic Acetylcholine Receptors Study
- Protein Kinase Regulation and GTPase Signaling
- Cellular Mechanics and Interactions
- Nitric Oxide and Endothelin Effects
- Photoreceptor and optogenetics research
- Neonatal Health and Biochemistry
- Ion Channels and Receptors
- Erythrocyte Function and Pathophysiology
- Rabbits: Nutrition, Reproduction, Health
- Ion Transport and Channel Regulation
Nagoya University
2013-2025
Osaka University
1975-2015
Foundation for Ichthyosis and Related Skin Types
2011-2012
Japan Science and Technology Agency
2005-2008
Kyoto Institute of Technology
1996-2000
National Institute for Physiological Sciences
1990-2000
National Institutes of Natural Sciences
1997
Nihon University
1997
Yamaguchi University
1996
Tokyo Medical and Dental University
1993
P-glycoprotein (Pgp), a drug transport protein, pumps many drugs out of hepatocytes. To begin to determine how variation in the level human hepatic Pgp might influence individual differences disposition, we have used Northern blot and immunochemical analysis mRNA for (MDR1) liver from 41 individuals. These samples were divided into two groups, normal perineoplastic (normal adjacent secondary neoplasms). There was large MDR1 protein expression between all samples. The average amount 2.5-fold...
Endothelial cells (ECs) release ATP in response to shear stress, a fluid mechanical force generated by flowing blood but, although its has crucial role controlling variety of vascular functions activating purinergic receptors, the mechanism never been established. To analyze dynamics release, we developed novel chemiluminescence imaging method using cell-surface-attached firefly luciferase and CCD camera. Upon stimulation cultured human pulmonary artery ECs simultaneously released two...
In patients with pulmonary diseases such as idiopathic fibrosis and severe acute respiratory distress syndrome, progressive is caused by dysregulated wound healing via activation of fibroblasts after lung inflammation or damage. Migration toward the fibrotic lesions plays an important role in fibrosis. Fibrotic tissue much stiffer than normal tissue. Emerging evidence supports hypothesis that stiffness matrix not only a consequence fibrosis, but also can induce fibroblast activation....
Cutaneous wound healing is accelerated by exogenous mechanical forces and impaired in TRPC6 knockout mice. Therefore, we designed experiments to determine how force channels contribute using HaCaT keratinocytes. cells were pretreated with hyperforin, a major component of traditional herbal medicine for also activator, cultured an elastic chamber. At 3 h after scratching on the confluent cells, ATP release intracellular Ca2+ increases response stretching (20%) live-imaged. was observed only...
Subepithelial fibroblasts form a cellular network just under the epithelium of gastrointestinal tract. Using primary cultured cells isolated from rat duodenal villi, we previously found that subepithelial reversibly changed cell morphology between flat and stellate-shape depending on intracellular cAMP levels. In this paper, examined cell-cell communication via released ATP Ca2+ signaling in network. were sensitive to mechanical stress such as ;touching' with fine glass rod ;stretching'...
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Abstract Astrocytes, a major subtype of glia, interact with neurons as supportive partner supplying energy sources and growth factors. Astrocytes regulate the activity neighboring by releasing chemical transmitters (gliotransmitters). However, precise role gilotransmitters in regulating neuronal is still under debate. Here, we report that subtle enhancement release one gliotransmitter, ATP, affects synaptic potentiation from an analysis mice containing astrocyte‐selective (GFAP) mutation. We...
The high interstitial ATP concentration in the cancer microenvironment is a major source of adenosine, which acts as strong immune suppressor. However, release has not been elucidated. We measured during hypotonic stress using real-time luminescence imaging system breast cell lines and primary cultured mammary cells. In lines, was released with slowly rising diffuse pattern, whereas cells, intermittently transient-sharp peaks. pattern changed to by cholera toxin treatment reverse change...
ABSTRACT A calcium ion indicator, fura-2 bovine serum albumin, was introduced into Dictyostelium discoideum cells by electroporation. The concentration of intracellular ions ([Ca2+]i) increased transiently in vegetative upon stimulation with submicromolar concentrations folic acid, a chemoattractant for this organism at the stage. Similar [Ca2+]i responses were also observed aggregation-competent subnanomolar cAMP, aggregation response caused cAMP 2.1 times higher than that acid. magnitude...
Abstract Mechano-transduction at cellular and tissue levels often involves ATP release activation of the purinergic signalling cascade. In lungs, stretch is an important physical stimulus but its impact on release, underlying mechanisms transduction pathways are poorly understood. Here, we investigated effect unidirectional from human alveolar A549 cells by real-time luciferin-luciferase bioluminescence imaging coupled with simultaneous infrared imaging, to monitor extent cell identify...
Activation of airway smooth muscle (ASM) cells plays a central role in the pathophysiology asthma. Because ASM is an important therapeutic target asthma, it beneficial to develop bioengineered models available for assessing physiological and biophysical properties cells. In condition vivo, are surrounded by extracellular matrix (ECM) exposed mechanical stresses such as cyclic stretch. We utilized 3-D culture model human embedded type-I collagen gel. further examined effects stretch, which...
Phase diagram has been determined with X-rays for bilayer membranes of mixed lipids dioleoyl-phosphatidylcholine/dipalmitoyl-phosphatidylcholine in water. Observed liquidus lines were reproduced well by calculation on the assumption ideal mixing two lipids. In fluid state there seems to be a transition temperature where small discontinuous structural change takes place. Obtained X-Ray data suggest that direction tilting lipid hydrocarbon chains becomes indefinite regard longitudinal angle...
Cutaneous wound healing is accelerated by mechanical stretching, and treatment with hyperforin, a major component of traditional herbal medicine known TRPC6 activator, further enhances the acceleration. We recently revealed that this was due to enhancement ATP-Ca 2+ signaling in keratinocytes hyperforin treatment. However, low aqueous solubility easy photodegradation impede topical application for therapeutic purposes. designed compound hydroxypropyl- β -cyclodextrin- (HP- -CD-) tetracapped...
An acute application of neurosteroid pregnenolone sulfate (PREGS) to hippocampal slices from adult rats induced a long-lasting potentiation (LLP PREGS) at the perforant path-granule cell synapse. As partial mechanism LLP PREGS, we previously revealed that PREGS transiently increases probability presynaptic glutamate release via sensitization alpha7-nicotinic acetylcholine receptor (alpha7nAChR). We herein demonstrate could be separated into two independent processes: above-mentioned early...