Abstract Background Trastuzumab resistance followed by metastasis is a major obstacle for improving the clinical outcome of patients with advanced human epidermal growth factor receptor 2-positive (HER-2+) breast cancer. While long non-coding RNAs (lncRNAs) can modulate cell behavior, contribution these in trastuzumab and HER-2+ cancer not well known. In this study, we sought to identify regulatory role lncRNA accompanied Epithelial-mesenchymal Transition (EMT) process Methods...

Accurate diagnosis and staging are crucial for selecting treatment patients with pancreatic ductal adenocarcinoma (PDAC). The desmoplastic responses associated PDAC often characterized by hypometabolism. Here, we investigated

This article introduces the concept of chiral ion mobility spectrometry (CIMS) and presents examples demonstrating gas-phase separation enantiomers a wide range racemates including pharmaceuticals, amino acids, carbohydrates. CIMS is similar to traditional spectrometry, where ions, when subjected potential gradient, are separated at atmospheric pressure due differences in their shapes sizes. In addition size shape, separates ions based on stereospecific interaction with gas. order achieve...

A secondary electrospray ionization (SESI) method was developed as a nonradioactive source for ion mobility spectrometry (IMS). This SESI relied on the gas-phase interaction between charged particles created by (ESI) and neutral gaseous sample molecules. Mass (MS) used detection after separation identification. Preliminary investigations focussed understanding process of SESI. The performance ESI-IMS SESI-IMS illicit drug evaluated determining analytical figures merit. In general, had higher...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMeasuring the Resolving Power of Ion Mobility SpectrometersWilliam F. Siems, Ching. Wu, Edward E. Tarver, Herbert H. Jr. Hill, Paul R. Larsen, and Dennis G. McMinnCite this: Anal. Chem. 1994, 66, 23, 4195–4201Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December 1994https://pubs.acs.org/doi/10.1021/ac00095a014https://doi.org/10.1021/ac00095a014research-articleACS PublicationsRequest...

A hybrid atmospheric pressure ion mobility spectrometer is described which exhibits resolving power approaching the diffusion limit for singly and multiply charged ions (over 200 most favorable case). Using an electrospray ionization source a downstream quadrupole mass with electron multiplier as detector, this ESI-IMS−MS instrument demonstrates potential of IMS rapid analytical separations similar to liquid chromatography. The first measurements gas-phase spectra mass-identified migrating...

Background and Purpose— This study investigated compared the responsiveness validity of Fugl-Meyer Assessment (FMA), Action Research Arm Test (ARAT), Wolf Motor Function (WMFT) for patients after stroke rehabilitation. Methods— A total 57 with received 1 3 rehabilitation treatments weeks. At pretreatment posttreatment, outcome measures, as well Functional Independence Measure (FIM) external criterion, were administered. The standardized response mean (SRM) Wilcoxon signed rank test used to...

In this paper, the first examples of baseline separation isomeric macromolecules by electrospray ionization/ion mobility spectrometry (ESI/IMS) at atmospheric pressure are presented. The behavior a number different peptides in IMS was investigated using nitrogen as drift gas. coupled to quadrupole mass spectrometer, which used for identification and selective detection electrosprayed ions. data were determine their average collision cross sections. gas-phase ions found have all cases, doubly...

Objectives. This study aimed to establish the minimal detectable change (MDC) and clinically important differences (CID) of Wolf Motor Function Test (WMFT) in patients with stroke, assess proportions patients' scores exceeding MDC CID after stroke rehabilitation. Methods. A total 57 received 1 3 treatments for weeks underwent clinical assessments before treatment. The MDC, at 90% confidence (MDC 90 ), was calculated from standard error measurement indicate a real individual patients....

The purpose of this study was to establish the minimal detectable change (MDC) and clinically important differences (CIDs) physical domains Stroke Impact Scale (SIS) assess proportions patients' scores exceeding MDC CIDs after stroke rehabilitation.Seventy-four patients received 1 3 treatments for weeks underwent clinical assessment before treatment. calculated from standard error measurement indicate a real with 95% confidence individual (MDC(95)). Anchor-based distribution-based approaches...

Objective: To compare the outcome of robot-assisted therapy with dose-matched active control by using accelerometers to study functional recovery in chronic stroke patients. Design: Prospective, randomized, controlled trial. Setting: Stroke units three medical centres. Subjects: Twenty patients post for a mean 22 months. Intervention: Robot-assisted ( n = 10) or 10). All received either these two therapies 90–105 minutes each day, 5 days per week, four weeks. Main measures: Outcome measures...

In this study, we compared the responsiveness and validity of Box Block Test (BBT), Nine-Hole Peg (NHPT), Action Research Arm (ARAT). We randomized 59 patients with stroke into one three rehabilitation treatments for 3 weeks. administered six outcome measures (BBT, NHPT, ARAT, Fugl-Meyer Assessment [FMA], Motor Activity Log [MAL], Stroke Impact Scale [SIS] hand function domain) pretreatment posttreatment. used standardized response mean (SRM) to examine Spearman rank correlation coefficient...

Background and Objective. This study investigated the relative effects of distributed constraint-induced therapy (CIT) bilateral arm training (BAT) on motor performance, daily function, functional use affected arm, quality life in patients with hemiparetic stroke. Methods. A total 60 were randomized to CIT, BAT, or a control intervention less specific but active therapy. Each group received intensive for 2 hours/day, 5 days/week, 3 weeks. Pretreatment posttreatment measures included...

Background and Objective. This study compared the efficacy of distributed constraint-induced therapy (dCIT), bilateral arm training (BAT), control treatment (CT) on motor functional performance upper limb in stroke patients. Methods. A total 66 patients with mean onset 16.20 months mild to moderate impairment were randomized dCIT, BAT, or CT groups. Each group received for 2 h/d 5 d/wk 3 weeks. Pretreatment posttreatment measures included reaching kinematic variables unilateral tasks, Wolf...

Cancer stem cells (CSCs) are predicted to be critical drivers of tumor progression due their "stemness", but the molecular mechanism CSCs in regulating metastasis remains elucidated. Epithelial-mesenchymal transition (EMT), hypoxia-inducible factor (HIF)-1α, and miR-21, all which contribute cell migration for metastasis, interrelated with CSCs. In present study, third-sphere forming (3-S) CSC-like cells, showed elevated CSC surface markers (ALDH1(+) CD44(+)/CD24(-/low)) sphereforming...

Spasticity is a major problem in patients with stroke and influences their activities of daily living, participation, quality life. The Modified Ashworth Scale widely used to assess spasticity. However, the responsiveness minimal clinically important differences have not been explored.This study aims examine stroke.Longitudinal six-month follow-up study.Rehabilitation wards tertiary hospital.One-hundred fifteen were recruited.All underwent assessment for upper extremity (flexors elbow,...

Persons with stroke frequently suffer from cognitive impairment. The Montreal Cognitive Assessment (MoCA), a recently developed screening tool, is sensitive to poststroke deficits. present study assessed its psychometric and clinimetric properties (i.e., responsiveness, minimal clinically important difference (MCID), criterion validity) in survivors receiving rehabilitative therapy.The MoCA the Stroke Impact Scale (SIS) were administered 65 before after 4 5 weeks of therapy. effect size...