A5057987858- Chemical Synthesis and Analysis
- Lymphatic System and Diseases
- Sphingolipid Metabolism and Signaling
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- PARP inhibition in cancer therapy
- Adenosine and Purinergic Signaling
- Cancer, Hypoxia, and Metabolism
- Enzyme Catalysis and Immobilization
- Glioma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Microbial Metabolic Engineering and Bioproduction
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Caveolin-1 and cellular processes
- Analytical Chemistry and Chromatography
- Endoplasmic Reticulum Stress and Disease
- Cancer Genomics and Diagnostics
- Asymmetric Synthesis and Catalysis
- Angiogenesis and VEGF in Cancer
- Silk-based biomaterials and applications
- Proteoglycans and glycosaminoglycans research
University of Turku
2015-2024
Institute for Environmental Sciences
2024
Osaka University
2011-2016
Kitasato University
2011-2014
Osaka International University
2013
Tokyo Medical University Ibaraki Medical Center
2013
Dankook University Hospital
2004
Kasumigaura Medical Center
2001
Showa Pharmaceutical University
1998
Okayama University
1975-1992
The lack of T cell infiltrates is a major obstacle to effective immunotherapy in cancer. Conversely, the formation tumor-associated tertiary-lymphoid-like structures (TA-TLLSs), which are local site humoral and cellular immune responses against cancers, associated with good prognosis, they have recently been detected checkpoint blockade (ICB)-responding patients. However, how these lymphoid aggregates develop remains poorly understood. By employing single-cell transcriptomics, endothelial...
Single-cell transcriptomics promises to revolutionize our understanding of the vasculature. Emerging computational methods applied high dimensional single cell data allow integration results between samples and species, illuminate diversity underlying developmental architectural organization populations. Here, we illustrate these in analysis mouse lymph node (LN) lymphatic endothelial cells (LEC) at resolution. Clustering identifies five well-delineated subsets, including two medullary sinus...
Lymphocyte extravasation from the high endothelial venules (HEVs) of lymph nodes is crucial for maintenance immune homeostasis, but its molecular mechanism remains largely unknown. In this article, we report that lymphocyte transmigration across basal lamina HEVs regulated, at least in part, by autotaxin (ATX) and end-product, lysophosphatidic acid (LPA). ATX an HEV-associated ectoenzyme produces LPA lysophosphatidylcholine (LPC), which abundant systemic circulation. agreement with selective...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAsymmetric reduction of the prochiral carbonyl group aliphatic .gamma.- and .delta.-keto acids by use fermenting bakers' yeastMasanori Utaka, Hisashi Watabu, Akira TakedaCite this: J. Org. Chem. 1987, 52, 19, 4363–4368Publication Date (Print):September 1, 1987Publication History Published online1 May 2002Published inissue 1 September 1987https://pubs.acs.org/doi/10.1021/jo00228a039https://doi.org/10.1021/jo00228a039research-articleACS...
Macrophages are critical in driving an immunosuppressive tumor microenvironment that counteracts the efficacy of T-cell-targeting therapies. Thus, agents able to reprogram macrophages toward a proinflammatory state hold promise as novel immunotherapies for solid cancers. Inhibition macrophage scavenger receptor Clever-1 has shown benefit inducing CD8
Lymph nodes (LNs) are highly confined environments with a cell-dense three-dimensional meshwork, in which lymphocyte migration is regulated by intracellular contractile proteins. However, the molecular cues directing intranodal cell remain poorly characterized. Here we demonstrate that lysophosphatidic acid (LPA) produced LN fibroblastic reticular cells (FRCs) acts locally to LPA2 induce T-cell motility. In vivo, either specific ablation of LPA-producing ectoenzyme autotaxin FRCs or...
Clever-1 also known as Stabilin-1 and FEEL-1 is a scavenger molecule expressed on subpopulation of anti-inflammatory macrophages lymphatic endothelial cells (LECs). However, its role in regulating dendritic cell (DC) trafficking subsequent effects immunity have remained unexplored. In this study, we demonstrate that DC from the skin into draining lymph nodes compromised absence Clever-1. By adoptive transfer approaches further show poor due to impaired entrance DCs afferent lymphatics....
In nonobese diabetic (NOD) mice, C. rodentium promotes priming of islet-specific T-cells in pancreatic lymph nodes (PaLN), which is a critical step initiation and perpetuation islet-autoimmunity. To investigate mechanisms by T-cell PaLN, we used fluorescent imaging lymphatic vasculature emanating from colon, followed dendritic cell (DC) migration colon using photoconvertible-reporter evaluated the translocation bacteria to with GFP-C. situ hybridization bacterial DNA. Fluorescent dextran...
Abstract Tissue engineering requires the delivery and survival of cells to organ sites needing repair. Previously, we showed that an active laminin peptide (AG73: RKR‐LQVQLSIRT)‐conjugated chitosan membrane promoted cell adhesion spreading in vitro . Here, seeded human keratinocytes onto AG73‐chitosan membranes found nearly 80% were attached within 2 h. The carrying inverted placed exposed muscle fascia on backs nude mice. After 3 days, had migrated from established a stratified...
Naive lymphocytes continuously migrate from the blood into lymph nodes (LNs) via high endothelial venules (HEVs). To extravasate HEVs, undergo multiple adhesion steps, including tethering, rolling, firm and transmigration. We previously showed that autotaxin (ATX), an enzyme generates lysophosphatidic acid (LPA), is highly expressed in ATX/LPA axis plays important role lymphocyte transmigration across HEVs. However, detailed mechanism underlying this axis's involvement has remained...
Radiation protection from bone marrow death by a single injection of partially purified ginseng extact after whole-body X-irradiation was confirmed in JCL-ICR mice. The extract efficacious both intraperitoneal and intravenous injection. protected mice when it injected 2 days before irradiation to 2.5 hr that. Recovery splenic weight DNA stimulated the extract, but that thymic not. Stimulated recovery also observed thrombocyte erythrocyte counts, while did not markedly affect leukocyte...
Abstract Reduction of ethyl 2-acetoxy-3-oxobutanoate (4a) with immobilized baker’s yeast in calcium alginate gel gave a 18 : 19 63 mixture (2R,3S)- and (2S,3S)-2-acetoxy-3-hydroxybutanoates (2S,3S)-2,3-dihydroxybutanoate each >95% e.e. 58% yield. A similar treatment 2-benzoyloxy analog 4a afforded 6 94 (2S,3S)-2-benzoyloxy-3-hydroxybutanoates (>95% e.e.) 70% Their absolute configurations were determined by comparison authentic (2S,3S)-2,3-dihydroxybutanoic acids. Effects the...
Vascular endothelial cells often change their phenotype to adapt local microenvironment. Here we report that the vascular adhesion molecule nepmucin/CD300LG, which is implicated in lymphocyte binding and transmigration, shows unique expression patterns microvascular of different tissues. Under physiological conditions, nepmucin/CD300LG was constitutively selectively expressed at luminal surface small arterioles, venules, capillaries most tissues, but it only weakly microvessels splenic red...
The present study aimed to develop a two-layered cultured dermal substitute (CDS). upper layer is hyaluronic acid (HA) and collagen (Col) spongy sheet with or without epidermal growth factor (EGF). lower HA Col gel containing fibroblasts. CDS prepared in serum-free medium, followed by placing on the wound surface. Corresponding clinical application, was incubated medium for period of 1, 3 5 days, onto air culture interface (wound surface model), 6 days using conventional supplemented serum....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRegio- and enantioselective reduction of .alpha.,2-dioxocycloalkaneacetates with fermenting baker's yeast. A new synthesis (R)-(-)-hexahydromandelic acidSadao Tsuboi, Emiko Nishiyama, Hiroyuki Furutani, Masanori Utaka, Akira TakedaCite this: J. Org. Chem. 1987, 52, 7, 1359–1362Publication Date (Print):April 1, 1987Publication History Published online1 May 2002Published inissue 1 April...
The autologous transplantation of hair follicles that have been separated into single follicular units is an accepted treatment for androgenetic alopecia. Recent studies demonstrate the multiple stem cell populations and surrounding cutaneous tissues coordinately regulate follicle functions skin homeostasis. Therefore, critical issues consideration regarding functional restoration therapy are reproduction correct connectivity cooperation with host tissues, including arrector pili muscle...
Lymphocyte entry into the spleen is initiated by their binding to Clever-1–expressing vessels in red pulp.
CD73 is an important ectoenzyme responsible for the production of extracellular adenosine. It involved in regulating inflammatory responses and cell migration overexpressed various cancers. The functions blood endothelial cells are understood detail, but its role on afferent lymphatics remains unknown. Moreover, anti-CD73 antibodies now used multiple clinical cancer trials, their effects different types have not been studied. This study reveals that a previously unknown to dampen immune...