A5037636317- RNA Research and Splicing
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Muscle Physiology and Disorders
- Protein Degradation and Inhibitors
- Telomeres, Telomerase, and Senescence
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Ovarian function and disorders
- Protein Tyrosine Phosphatases
- Reproductive Biology and Fertility
- DNA and Nucleic Acid Chemistry
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Ovarian cancer diagnosis and treatment
- Multiple Myeloma Research and Treatments
- Chromatin Remodeling and Cancer
- Genomic variations and chromosomal abnormalities
- Nitrogen and Sulfur Effects on Brassica
- Diphtheria, Corynebacterium, and Tetanus
- Cancer Research and Treatments
- Cardiac Fibrosis and Remodeling
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Mechanisms of cancer metastasis
Jinzhou Medical University
2024-2025
Hong Kong University of Science and Technology
2020-2023
University of Hong Kong
2020-2023
Hiroshima University
2021
North China University of Science and Technology Affiliated Hospital
2020
Significance Epigenetic regulations control the accessibility of transcription factors to their target regions. Modulation chromatin determines which transcripts be expressed and therefore, defines cell identity. Chromatin modulation during fate determination involves a complex regulatory network, yet comprehensive view remains explored. Here, we provide global muscle stem activation. We identified long noncoding RNA (lncRNA), LncMyoD , regulates lineage progression through modulating...
Age-associated impairments in adult stem cell functions correlate with a decline somatic tissue regeneration capacity. However, the mechanisms underlying molecular regulation of aging remain elusive. Here, we provide proteomic analysis physiologically aged murine muscle cells (MuSCs), illustrating pre-senescent signature. During aging, mitochondrial proteome and activity are impaired MuSCs. In addition, inhibition function results cellular senescence. We identified an RNA-binding protein,...
Abstract Bmp plays an important role in cardiomyocyte differentiation, but the function of Smad4 signaling remains elusive. Here, we show that disruption gene cardiac progenitors expressing Sfrp5 led to embryonic lethality with hypoplastic heart formation. Although expression Nkx2-5 is regulated by signaling, was weakly detected mutant heart. However, nuclear translocation impaired. Expression CK2 or PP1 , which could alter phosphorylation status NLS Nkx2-5, not affected, found bind...
Chromatin accessibility is critical for cell identity. Conventional ATAC-seq can examine chromatin on freshly prepared muscle stem cells or satellite (SCs); however, isolating SCs in mice remains challenging. Here, we present a protocol to preserve the vivo profile of by applying paraformaldehyde (PFA) perfusion throughout mouse before SC isolation. We describe steps PFA and FACS sorting SCs. then detail library preparation ATAC-seq. For complete details use execution this protocol, please...
Abstract Background Polycystic Povary syndrome(PCOS) is a diverse condition with an unknown cause. Anti-Mullerian hormone(AMH) hormone that belongs to the transforming growth factor-β(TGF-β) class. Mothers against decapentaplegic homolog 4(SMAD4) crucial transcription factor widely expressed in granulosa cells TGF-β signaling pathway. Previous studies have revealed AMH may be important follicular developmental disorders PCOS patients , as biomarker of PCOS. Objective This study examines...
Regulation of chromatin accessibility is critical for cell fate decisions. Chromatin structure responds to extrinsic environments rapidly. The traditional adult stem isolation approach requires tissue dissociation, which triggers activation and leads alterations in structure. To preserve the vivo states, we utilized PFA-perfusion-based characterized DNA regulatory landscapes during muscle quiescence exit aging. We showed that aged SCs display a chronically activated signature. Detailed...