A5101602639- Osteoarthritis Treatment and Mechanisms
- RNA Interference and Gene Delivery
- Knee injuries and reconstruction techniques
- Total Knee Arthroplasty Outcomes
- Lower Extremity Biomechanics and Pathologies
- Advanced Drug Delivery Systems
- Neuroscience and Neural Engineering
- Antimicrobial Peptides and Activities
- Advanced biosensing and bioanalysis techniques
- Inflammatory mediators and NSAID effects
- Clinical Nutrition and Gastroenterology
- Nanofabrication and Lithography Techniques
- Lubricants and Their Additives
- Tissue Engineering and Regenerative Medicine
- Advanced Sensor and Energy Harvesting Materials
- Analytical Methods in Pharmaceuticals
- Polymer Surface Interaction Studies
- Force Microscopy Techniques and Applications
- Biochemical and Structural Characterization
- Advanced Biosensing Techniques and Applications
- Exercise and Physiological Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Bone and Joint Diseases
- Vitamin D Research Studies
- Extracellular vesicles in disease
Northeastern University
2017-2025
Universidad del Noreste
2020-2022
IIT@MIT
2013-2015
Massachusetts Institute of Technology
2013-2014
ABSTRACT Intra‐articular (i.a.) drug delivery for local treatment of osteoarthritis remains inadequate due to rapid clearance by the vasculature or lymphatics. Local therapy targeting articular cartilage is further complicated its dense meshwork collagen and negatively charged proteoglycans, which can prevent even nano‐sized solutes from entering. In a previous in vitro study, we showed that Avidin, size (7 nm diameter) high positive charge (p I 10.5), penetrated full thickness bovine was...
Abstract For evaluation of new approaches to drug delivery into cartilage, the choice an animal model is critically important. Since cartilage thickness varies with size, different levels uptake, transport and retention should be expected. Simple intra‐articular injection can require very high doses achieve a concentration gradient enough for diffusion cartilage. New involve nanoparticle functionalized drugs directly cartilage; however, diffusion‐binding kinetics proceeds as square . In this...
Abstract Background Most in vitro studies of potential osteoarthritis (OA) therapies have used cartilage monocultures, even though synovium is a key player mediating joint inflammation and, thereby, degeneration. In the case interleukin-1 (IL-1) inhibition using its receptor antagonist (IL-1Ra), like chondrocytes, synoviocytes also express IL-1 receptors that influence intra-articular signaling and IL-1Ra efficacy. The short residence time after injection requires application frequent...
Biological systems overwhelmingly comprise charged entities generating electrical activity that can have significant impact on biological structure and function. This intrinsic bio-electrical also be harnessed for overcoming the tissue matrix cell membrane barriers, which been outstanding challenges targeted drug delivery, by using rationally designed cationic carriers. The weak reversible long-range electrostatic interactions with fixed negatively groups facilitate electro-diffusive...
Here we describe methods for synthesizing a cationic, multi-arm Avidin (mAv) nano-construct that has wide range of applications in drug delivery and imaging negatively charged tissues. We use Avidin-biotin technology gives the flexibility conjugating biotinylated Dexamethasone to mAv by simple mixing at room temperature. also control hydrolysis rates ester linkers enable sustained (and tunable) release therapeutic doses.•Multi-arm structure provides multiple sites covalent conjugation...
Abstract Low back pain is often the direct result of degeneration intervertebral disc. A wide range therapeutics including anti-catabolic, pro-anabolic factors and chemo-attractants that can stimulate resident cells recruit endogenous progenitors are under consideration. The avascular nature dense matrix this tissue make it challenging for systemically administered drugs to reach their target inside nucleus pulposus (NP), central gelatinous region disc (IVD). Therefore, local intra-discal...
Several negatively charged tissues in the body, like cartilage, present a barrier to targeted drug delivery due their high density of aggrecans and, therefore, require improved targeting methods increase therapeutic response. Because cartilage has negative fixed charge density, drugs can be modified with positively carriers take advantage electrostatic interactions, allowing for enhanced intra-cartilage transport. Studying transport is, crucial towards predicting efficacy inducing biological...
Abstract Gastric resident dosage forms have been used successfully in farm animals for the delivery of a variety drugs helping address challenge extended dosing. Despite these advances, there remains significant across range species with large variation body size. To this, we investigate scalable gastric platform capable prolonged retention. We prototypes dimensions consistent administration and retention stomachs two (rabbit pig). sustained our form platform, pigs show capacity to modulate...
Several negatively charged tissues in the body, like cartilage, present a barrier to targeted drug delivery due their high density of aggrecans and, therefore, require improved targeting methods increase therapeutic response. Because cartilage has negative fixed charge density, drugs can be modified with positively carriers take advantage electrostatic interactions, allowing for enhanced intra-cartilage transport. Studying transport is, crucial towards predicting efficacy inducing biological...
Biphasic poro-viscoelastic constitutive material model (BPVE) captures both the fluid flow dependent and independent behavior of cartilage under stress relaxation type indentation. A finite element based on BPVE formulation was developed to explore sensitivity Young's modulus, Poisson's ratio, permeability, viscoelastic parameters expressed in terms Prony series coefficients. Then we fit numerical experimental force versus time curves from indents bovine tibial plateaus extract properties....
Abstract Adolescent obesity has risen dramatically in the last few decades. While adult may be osteoprotective, effects of during adolescence, which is a period massive bone accrual, are not clear. We used murine model induced adolescent to examine structural, mechanical, and compositional differences between obese healthy weight 16‐week‐old female C57Bl6 mice. also examined return normal after skeletal maturity (24 weeks old). found exhibited decreased trabecular volume, increased cortical...