A5102705047- Nematode management and characterization studies
- Cytokine Signaling Pathways and Interactions
- Lung Cancer Diagnosis and Treatment
- Genetics, Aging, and Longevity in Model Organisms
- COVID-19 Clinical Research Studies
- Global Cancer Incidence and Screening
- Head and Neck Cancer Studies
- SARS-CoV-2 and COVID-19 Research
- Legume Nitrogen Fixing Symbiosis
- Wound Healing and Treatments
- Long-Term Effects of COVID-19
- Cancer Immunotherapy and Biomarkers
- Respiratory Support and Mechanisms
- Heat shock proteins research
- Lung Cancer Treatments and Mutations
- Pressure Ulcer Prevention and Management
- Reproductive System and Pregnancy
- Airway Management and Intubation Techniques
- Pneumonia and Respiratory Infections
- Selenium in Biological Systems
- Immune Cell Function and Interaction
- HIV/AIDS Research and Interventions
- Plant Pathogens and Resistance
- Bacterial biofilms and quorum sensing
- IL-33, ST2, and ILC Pathways
University of Leeds
2011-2025
University of Toronto
2024
Oxford University Clinical Research Unit
2021
NIHR Clinical Research Network
2021
National Institute for Health Research
2021
National Records of Scotland
2021
Intensive Care National Audit & Research Centre
2021
Public Health Scotland
2021
University Hospitals Birmingham NHS Foundation Trust
2021
Public Health England
2021
Abstract Background Globodera pallida is a devastating pathogen of potato crops, making it one the most economically important plant parasitic nematodes. It also an model for biology cyst Cyst nematodes and root-knot are two nematode groups together represent global threat to food security. Results We present complete genome sequence G. , with transcriptomic data from life cycle, particularly focusing on cycle stages involved in root invasion establishment biotrophic feeding site. Despite...
The yellow potato cyst nematode, Globodera rostochiensis, is a devastating plant pathogen of global economic importance. This biotrophic parasite secretes effectors from pharyngeal glands, some which were acquired by horizontal gene transfer, to manipulate host processes and promote parasitism. G. rostochiensis classified into pathotypes with different resistance-breaking phenotypes. We generate high quality genome assembly for pathotype Ro1, identify putative transfer events, map expression...
Powdery mildew, caused by the obligate biotrophic fungus Erysiphe necator, is an economically important disease of grapevines worldwide. Large quantities fungicides are used for its control, accelerating incidence fungicide-resistance. Copy number variations (CNVs) unbalanced changes in structure genome that have been associated with complex traits. In addition to providing first description large and highly repetitive E. this study describes impact genomic structural variation on fungicide...
Immunosurveillance constitutes the first step of cancer immunoediting in which developing malignant lesions are eliminated by antitumorigenic immune cells. However, mechanisms neoplastic cells induce an immunosuppressive state to evade response still unclear. The transcription factor STAT3 has been implicated breast carcinogenesis and tumor immunosuppression advanced disease, but its involvement early disease development not established. Here, we genetically ablated Stat3 epithelia inducible...
Plant pathogens and parasites are a major threat to global food security. parasitism has arisen four times independently within the phylum Nematoda, resulting in at least one parasite of every crop world. Some species most economically important order (Tylenchida) secrete proteins termed effectors into their host during infection re-programme development immunity. The precise detail how nematodes evolve new is not clear. Here we reconstruct evolutionary history novel effector gene family. We...
Aim: Fluorescence imaging can visualize polymicrobial populations in chronic and acute wounds based on porphyrin fluorescence. We investigated the fluorescent properties of specific wound pathogens fluorescence detected from bacteria biofilm. Methods: Utilizing Remel Porphyrin Test Agar, 32 bacterial four yeast species were examined for red under 405 nm violet light illumination. Polymicrobial biofilms, supplemented with δ-aminolevulinic acid, similarly. Results: A total 28/32 bacteria, 1/4...
Abstract The potato cyst nematodes Globodera pallida and G. rostochiensis are economically important plant pathogens causing losses to UK harvests estimated at £50 m/ year. Implications of climate change on their future pest status have not been fully considered. Here, we report growth female over the range 15 25°C. Females per fecundity declined progressively with temperatures above 17.5°C for , whilst females were optimal between 17.5 22.5°C . Relative reproductive success temperature was...
Grape powdery mildew (PM), caused by the biotrophic ascomycete Erysiphe necator, is a devastating fungal disease that affects most Vitis vinifera cultivars. We have previously identified panel of V. accessions from Central Asia with partial resistance to PM possess Ren1-like local haplotype. In this study, we show in addition typical Ren1-associated late post-penetration resistance, these display range different levels development suggesting alternative alleles or additional genes contribute...
Characterisation of the pathways by which xenobiotics are metabolised and excreted in both target non-target organisms is crucial for rational design effective specific novel bioactive molecules. Consequently, we have investigated induced responses model nematode Caenorhabditis elegans to a variety represent range putative modes action. The majority genes that were specifically preliminary microarray analyses encoded enzymes from Phase I II metabolism, including cytochrome P450s, short chain...
Objective: Diagnostics which provide objective information to facilitate evidence-based treatment decisions could improve the chance of wound healing. Accurate measurements, bacterial assessment, and regular, consistent tracking these parameters are important aspects care. This study aimed assess accuracy, clinical incorporation documentation capabilities a handheld fluorescence imaging device (MolecuLight i:X). Method: Benchtop models with known dimensions images were repeatedly measured by...
Wound biofilms must be identified to target disruption and bacterial eradication but are challenging detect with standard clinical assessment. This study tested whether fluorescence imaging could porphyrin-producing bacteria within a biofilm using well-established in vivo models. Mouse wounds were inoculated on Day 0 planktonic (n = 39, non-porphyrin-producing species, 107 colony forming units (CFU)/wound) or polymicrobial 16, 3 per mouse, each 1:1:1 parts Staphylococcus aureus/Escherichia...
Hydrophobic interaction chromatography (HIC) was used to separate populations of recombinant IgG2 antibody that were created as a result prolonged incubation at 40 degrees C. Antibody separated by HIC into three major and seven minor fractions. All but one fraction composed with distinct chemical modifications resulted from exposure elevated temperature. The results intact reduced mass analysis well peptide map data derived the fractions indicated being chromatographically containing...
Control of Fasciola hepatica infections livestock in the absence vaccines depends largely on chemical triclabendazole (TCBZ) because it is effective against immature and adult parasites. Overdependence a single drug improper application considered significant factor increasing global reports fluke resistant to TCBZ. The mode(s) action biological target(s) TCBZ are not confirmed, delaying detection monitoring early resistance. In this study, further understand liver response TCBZ, soluble...
Abstract Rab coupling protein (FIP1C), an effector of the Rab11 GTPases, including Rab25, is amplified and overexpressed in 10% to 25% primary breast cancers correlates with poor clinical outcome. Rab25 also frequently silenced triple-negative cancer, suggesting its ability function as either oncogene or a tumor suppressor, depending on cancer subtype. However, pathobiologic role FIP family members, such FIP1C, tumor-specific setting remains elusive. In this study, we used ErbB2 mouse models...
Background Breast milk transmission of HIV-1 remains a major route pediatric infection. Defining the characteristics viral variants to which breastfeeding infants are exposed is important for understanding genetic bottleneck that occurs in majority mother-to-child transmissions. The blood-milk epithelial barrier markedly restricts quantity breast milk, even absence antiretroviral drugs. basis this restriction and relationship between blood not well established. Methodology/Principal Findings...
High bacterial loads within chronic wounds increase the risk of infection and complication. Detection localization through point-of-care fluorescence (FL) imaging can objectively inform support treatment decisions. This single time-point, retrospective analysis describes decisions made on 1000 (DFUs, VLUs, PIs, surgical wounds, burns, others) at 211 wound-care facilities across 36 US states. Clinical assessment findings plans derived from them, as well subsequent FL-imaging (MolecuLight®)...
BACKGROUND Hospitalized COVID-19 patients with troponin elevation have a higher prevalence of cardiac abnormalities than control individuals.However, the progression and impact myocardial injury on survivors remain unclear.OBJECTIVES This study sought to evaluate in baseline follow-up imaging assess medium-term outcomes.METHODS was prospective, longitudinal cohort 25 United Kingdom centers (June 2020 March 2021).Hospitalized underwent magnetic resonance (CMR) scans within 28 days 6 months...
Stress responses are controlled by specialized stress-responsive proteostasis transcription factors that rapidly upregulate protein quality components to re-establish homeostasis and safeguard survival. Here we show the zinc finger factor PQM-1 is crucial for stress survival in response thermal oxidative challenges. We provide mechanistic insight into regulation of during depends on ILS-DAF-16 signaling, as well phosphorylation threonine residue 268 located within a conserved AKT motif. Our...
EPR spectra at 94.5 GHz (3 mm) and 249.9 (1 have been measured for the dichloro-, dibromo-, diiodobis(triphenylphosphine oxide)manganese(II) complexes. These compounds a distorted tetrahedral geometry, highest possible symmetry obtainable is C2v. Because are taken in high-field limit, they simple much easier to interpret than lower magnetic fields. Accurate values of zero-field splitting parameters, D η (E/D), determined by least-squares fitting homotopy using matrix diagonalization. The...
Summary An oxygen‐enriched atmosphere enhances the potential for operating‐room fires. We thus determined oxygen concentrations at various facial landmarks during administration via nasal cannulae. Thirteen supine volunteers were draped similarly to patients undergoing a cervical‐node biopsy. Oxygen was delivered in random order through cannulae rates of 2, 4, and 6 l.min −1 . concentration measured pre‐determined also distal drape non‐facial sites. At flow 2 , exceeded 23% only within few...
Knowledge of how drugs are metabolized and excreted is an essential component understanding their fate within among target non-target organisms. Thiabendazole (TBZ) was the first benzimidazole (BZ) to be commercially available remains one most important anthelmintic for medical veterinary use. We have characterized Caenorhabditis elegans metabolizes excretes TBZ. shown that TBZ directly binds nuclear hormone receptor (NHR)-176 this required induction by cytochrome P450 (CYP) encoded...
Cyst nematodes are a group of plant pathogens each with defined host range that cause major losses to crops including potato, soybean and sugar beet. The infective mobile stage hatches from dormant eggs moves short distance through the soil roots, which it then invades. A novel strategy for control has recently been proposed in is able secrete peptide disorientates prevents invasion pathogen. This study provides indirect evidence support mechanism by one such disrupts chemosensory function...