A5028068838- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Protein Kinase Regulation and GTPase Signaling
- Protein Tyrosine Phosphatases
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- Biochemical effects in animals
- Receptor Mechanisms and Signaling
- Free Radicals and Antioxidants
- Mass Spectrometry Techniques and Applications
- Cytokine Signaling Pathways and Interactions
- Antimicrobial Peptides and Activities
- Carbohydrate Chemistry and Synthesis
- Biochemical and Structural Characterization
- Analytical Chemistry and Chromatography
- Protein Hydrolysis and Bioactive Peptides
- Click Chemistry and Applications
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Radiopharmaceutical Chemistry and Applications
- Neuropeptides and Animal Physiology
- X-ray Diffraction in Crystallography
- PI3K/AKT/mTOR signaling in cancer
- Enzyme Structure and Function
- Melanoma and MAPK Pathways
Istituto di Chimica Biomolecolare
2002-2019
University of Padua
2000-2013
Weatherford College
2009-2011
National Institute on Aging
2005
Institute on Aging
2005
A tissue-specific casein kinase, purified from the Golgi-enriched-membrane fraction of guinea-pig lactating mammary gland (GEF-CK), readily phosphorylates synthetic peptide Ser-Glu5, a good substrate kinase-2, and several derivatives varying for number position acidic residues on C-terminal side serine, except those lacking an chain at +2. The least peptide, still significantly affected by GEF-CK, is Ser-Ala-Glu-Ala3 which not CK-2. Conversely, peptides Ser-Ala2-Glu-Ala2, Ser-Ala2-Glu3,...
Abstract Histidine is a naturally occurring amino acid with antioxidant properties, which present in low amounts tissues throughout the body. We recently synthesized and characterized histidine analogues related to natural dipeptide carnosine, selectively scavenge toxic lipid peroxidation product 4‐hydroxynonenal (HNE). now report that analogue histidyl hydrazide effective reducing brain damage improving functional outcome mouse model of focal ischemic stroke when administered intravenously...
Antamanide is a cyclic decapeptide derived from the fungus Amanita phalloides. Here we show that antamanide inhibits mitochondrial permeability transition pore, central effector of cell death induction, by targeting pore regulator cyclophilin D. Indeed, (i) inhibition not additive with D-binding drug cyclosporin A, (ii) inhibitory action on requires phosphate, as previously shown for A; (iii) ineffective in mitochondria or cells D null animals, and (iv) abolishes CyP-D peptidyl-prolyl...
Glutathione transferase (formerly GST) catalyzes the inactivation of various electrophile-producing anticancer agents via conjugation to tripeptide glutathione. Moreover, several data link overexpression some GSTs, in particular GSTP1-1, both natural and acquired resistance structurally unrelated drugs. Tumor these proteins has provided a rationale for search GST inhibitors activated cytotoxic prodrugs. In present review we discuss current structural pharmacological knowledge GST-activated compounds.
The local specificity determinants for a T‐cell protein tyrosine phosphatase (TC‐PTP) have been inspected with the aid of series synthetic peptides, either enzymically or chemically phosphorylated, derived from phosphoacceptor sites phosphotyrosyl proteins. truncated form PTP, deprived its C‐terminal down‐regulatory domain, readily dephosphorylates submicromolar concentrations eptapeptides to eicosapeptides, reproducing site pp60 c‐src (Tyr527), phosphorylated loop IV calmodulin and main...
Many neurodegenerative diseases, including Huntington’s, Alzheimer’s and Parkinson’s are characterized by protein misfolding aggregation. The capability of trehalose to interfere with aggregation has been recently evaluated several research groups. In the present work, we studied, means synchrotron radiation circular dichroism (SRCD) spectroscopy, dose-effect on α-synuclein conformation and/or stability probe this osmolyte α-synuclein’s Our study indicated that a low concentration stabilized...
Abstract Polyoxometalates (POMs) are multi metallic and polyanionic oxides whose functionalization with organic moieties can generate new properties. Among possible strategies to molecular diversity find applications in different fields, the postfunctionalization of a POM peptides is particularly interesting easily achievable. In this article, we present Anderson‐Evans polyoxomolybdate ([MnMo 6 O 24 ] 3– ) Bombesin antagonist peptide, highlight interplay between these 2 domains, terms...
A growing number of natural and/or synthetic peptides with cell membrane penetrating capability have been identified and described in the past years. These molecules considered promising tools for delivering bioactive compounds into various types. Although mechanism uptake is still unclear, it reasonable to assume that relative contribute each proposed could differ same peptide, depending on experimental protocol cargo molecule composition. In this work we try connect interact model lipid...
Abstract Tat cell‐penetrating peptide (GRKKRRQRRRPPQG) is able to translocate and carry molecules across cell membranes. Using CD spectroscopy the conformation of this synthetic was studied in aqueous membrane‐mimicking, micellar SDS solutions at different temperatures. The spectrum solution virtually unchanged from that solution, low temperature it close a poly(proline) II helix. Copyright © 2004 European Peptide Society John Wiley & Sons, Ltd.
The synthesis, scavenging activity, and cytoprotective profiles of histidyl-containing carnosine analogues bearing hydrazide or 1,2-diol moieties is reported. Some compounds have demonstrated higher aldehyde-sequestering efficiency than were also efficient in protecting SH-SY5Y neuroblastoma cells rat hippocampal neurons from 4-hydroxy-trans-2,3-nonenal (HNE)-mediated death. efficacy these suggests their potential use as therapeutic agents for disorders that involve excessive membrane lipids...
Eukaryotic signal transduction involves the assembly of transient protein-protein complexes mediated by modular interaction domains. Specific Pro-rich sequences with consensus core motif PxxP adopt PPII helix conformation upon binding to SH3 For short peptides, little or no ordered secondary structure is usually observed before interactions. The association a peptide domain unfavorable entropy due loss rotational freedom on forming helix. With aim stabilizing in HPK1 decapeptide PPPLPPKPKF...
Abstract The conformational and ion binding properties of the sequences 93–104, 96–104, 93–98 domain III bovine brain calmodulin (CaM) have been studied by CD Tb 3+ ‐mediated fluorescence. In aqueous solution interaction all fragments with Ca 2+ Mg ions is very weak without any effect on peptide conformation, which remains always random. trifluoroethanol strong different exhibit distinct properties. particular, dodecapeptide fragment its N‐terminal hexapeptide 98–104, bind calcium magnesium...
Abstract Cortactin is a ubiquitous actin‐binding protein that regulates various aspects of cell dynamics and implicated in the pathogenesis human neoplasia. The sequence cortactin contains number signaling motifs an SH3 domain at C‐terminus, which mediates interaction with several partners, including Shank2. A recombinant protein, comprising murine fused to GST (GST‐SH3 m‐cort ), was prepared used assess domain‐binding affinity potential peptide‐ligands reproducing proline‐rich regions HPK1...
Targeted molecular imaging techniques have become indispensable tools in modern diagnostic, providing accurate and specific disease information. Conventional non-specific contrast agents suffer from low targeting efficiency; thus, the use of molecularly targeted probes is needed depending on different modalities. The overexpression peptide receptors many human cancers has attracted an enormous interest molecules for development tumor-specific radiopharmaceutical compounds diagnostic therapy...
Abstract: In order to investigate the antiproliferative properties of antamanide, we have synthesized and studied two antamanide analogs where phenylalanine residue in positions 6 or 9 is substituted by tyrosine, their corresponding linear forms cyclic des Phe 5 ,Phe –Tyr –analogs. Antamanide its biologically active synthetic are able form highly stable complexes with metal ions, particularly Na + , K Ca 2+ . We ion‐binding Tyr–antamanide CD Tb 3+ ‐mediated fluorescence acetonitrile. this...
Despite the very recent discovery that about 25% of apparently sporadic forms pheochromocytoma are actually due to germline mutations RET, VHL, SDHB or SDHD genes, genetic bases tumourigenesis this type cancer still incompletely understood. Recent studies provided evidence a new tumour suppressor gene, mapping on short arm chromosome 1, could be involved in early pheochromocytoma.We have performed fine analysis loss heterozygosity (LOH) region. In particular, we analysed 31 highly...
Abstract Cathepsin B is a cysteine protease that in tumor tissues localized both acidic lysosomes and extracellular spaces. It can catalyze the cleavage of peptide bonds by two mechanisms: endoproteolytic attack with pH optimum around 7.4, from C ‐terminus at 4.5–5.5. In this work, seven fluorescent, internally quenched, decapeptides have been synthesized using prototypical cathepsin selective substrate Z‐Phe‐Arg‐AMC as lead, used to identify structural factors determining susceptibility...