A5029136654- Advanced Fluorescence Microscopy Techniques
- Blood properties and coagulation
- Photoacoustic and Ultrasonic Imaging
- Erythrocyte Function and Pathophysiology
- Microfluidic and Bio-sensing Technologies
- Laser Material Processing Techniques
- Lipid Membrane Structure and Behavior
- Advancements in Transdermal Drug Delivery
- Optical Imaging and Spectroscopy Techniques
- 3D Printing in Biomedical Research
- Curcumin's Biomedical Applications
- Osteoarthritis Treatment and Mechanisms
- Collagen: Extraction and Characterization
- Advanced optical system design
- Magnetic and Electromagnetic Effects
- Adaptive optics and wavefront sensing
- Micro and Nano Robotics
- Polymer Foaming and Composites
- Silk-based biomaterials and applications
- Hemoglobin structure and function
- Retinoids in leukemia and cellular processes
- Spectroscopy Techniques in Biomedical and Chemical Research
- Laser Applications in Dentistry and Medicine
- Tissue Engineering and Regenerative Medicine
- Advanced Biosensing Techniques and Applications
Central Scientific Research Institute of Traumatology and Orthopedics
2017-2024
Ministry of Health of the Russian Federation
2017
State Optical Institute named after SI Vavilov
1994
Extracellular matrix (ECM) provides both structural support and dynamic microenvironment for cells regulating their behavior fate. As a critical component of stem cell niche ECM maintains activates proliferation differentiation under specific stimuli. Mesenchymal stem/stromal (MSCs) regulate tissue-specific functions locating in immediate producing various bioactive factors, including components. We evaluated the ability MSC-produced to restore progenitor vitro analyzed possible mechanisms...
Blood cell analysis is one of the standard clinical tests.Despite widespread use exogenous markers for blood quantification, label-free optical methods are still high demand due to their possibility in vivo application and signal specific biochemical state provided by native fluorophores.Here we report results characterization using fluorescence imaging techniques flow-cytometry.Autofluorescence parameters different types -white cells, red erythrophagocytic cells -are assessed analyzed terms...
Developing bone scaffolds can greatly improve the patient's quality of life by accelerating rehabilitation process. In this paper, we studied process composite polycaprolactone supercritical foaming for tissue engineering. The influence graphene oxide and reduced on parameters was studied. structural mechanical properties were demonstrated flexibility endurance. co-culturing live/dead tests that obtained are biocompatible. Different induced various surface cell behaviors. experimental data...
Objective Chondrospheres represent a variant of tissue spheroids biofabricated from chondrocytes. They are already being used in clinical trials for cartilage repair; however, their biomechanical properties have not been systematically investigated yet. The aim our study was to characterize chondrospheres long-term vitro culture conditions morphometric changes, integrity, and fusion spreading kinetics. Results It has demonstrated that the increase secant modulus elasticity is strongly...
Two-photon excited fluorescence of red blood cells (RBC) has been reported to be applicable for their assessment in vitro and vivo. The corresponding emission was ascribed hemoglobin (Hb), however, as Hb is essentially non-fluorescent at single-photon excitation, the mechanism two-photon RBC remains debatable. Here we show that a fluorescent photoproduct, characterized by an ultrafast decay formed after irradiation with femtosecond laser pulses ca. 8 · 10−5 quantum yield, it also excitation....
Abstract In this study, fluorescence recovery after photobleaching (FRAP) experiments were performed on RBC labeled by lipophilic fluorescent dye CM-DiI to evaluate the role of adenylyl cyclase cascade activation in changes lateral diffusion erythrocytes membrane lipids. Stimulation adrenergic receptors with epinephrine (adrenaline) or metaproterenol led significant acceleration FRAP recovery, thus indicating an elevated fluidity. The effect stimulation protein kinase A membrane-permeable...
Abstract Investigation of cells and tissues in vitro systems is an attempt to simplify the very complex interactions between various cell types from multicellular organisms. Monolayer cultures with single type do not allow show all possible paracrine cells. To analyze intercellular inter-actions, it use that co-cultivate several types. This article proposes a new co-cultivation system based on levitation magnetic field culture medium protein membrane The developed can be made any laboratory...
Various technologies based on different light—tissue interaction phenomena have been developed for medical applications. Now the progress in area of laser depends more and from design a special fiber—optical tools1'2 . Thus flexible fabrication technique such fiber tools manufacturing is needed These should serve beam forming ( local spot or volume irradiation, inclination irradiation direction, size intensity distribution variation, etc.), most effective use power distinct operations....
A new approach to fiber—end microlenses fabrication is described below. The general idea combine laser action on glass samples ( for example, fiber ) with such mechanical forces as stretching, twisting, bending, etc. Several different schemes combination are suggested and tested'. next innovation observe measure microlens geometrical optical parameters during the processing use results control production. This allows one producing measurement testing operations in time place , that provide...
The possibility to use oral intake of fluorescent dyes approved on humans for intravital fluorescence in vivo imaging is attractive due its simplicity and non-invasive character. Here we investigate the potential solid lipid curcumin particles (SLCP) capillary permeability using video capillaroscopy. Curcumin corresponding 0.7 μg/ml concentration was observed from blood plasma after consumption 2 g SLCP. However, this signal ~ 15% higher than that intrinsic fluorescence, which itself at...
The possibility of using spectrally and time-resolved endogenous fluorescent response to monitor processes involving white blood cells was investigated.
Analysis of laser-assisted 3D-LOM systems is given. Parameters lasers and laser beam delivery as scanners plotters are estimated. Criteria optimization them suggested. Characteristics two designed LOM Optoacoustical device OASCAN for complicated 3D-objects images input described.
Problems are considered referring to development of a large adaptive telescope consisting individual modules with common image and synthesized aperture. The capabilities estimated by Fresnel number as product aperture size field dimensions divided wavelength. It is shown that configurations based on `power' most promising ones provide superlarge values rather than conventional afocal modules. Some problems solved including matching using the images curved surfaces having center simultaneous...