A5083708850- Helicobacter pylori-related gastroenterology studies
- Microscopic Colitis
- Diet and metabolism studies
- Gastrointestinal motility and disorders
- Legionella and Acanthamoeba research
- Cancer, Stress, Anesthesia, and Immune Response
- Inflammatory Bowel Disease
- Gut microbiota and health
- Clinical Nutrition and Gastroenterology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Gastroesophageal reflux and treatments
- Esophageal and GI Pathology
- Gastrointestinal disorders and treatments
- Diverticular Disease and Complications
- Lymphatic System and Diseases
- Cancer Diagnosis and Treatment
- Bacterial biofilms and quorum sensing
- Diabetes Treatment and Management
- Liver Disease Diagnosis and Treatment
- Biliary and Gastrointestinal Fistulas
- Diagnosis and treatment of tuberculosis
- Medical and Biological Ozone Research
- Vibrio bacteria research studies
- Digestive system and related health
Self-Defense Forces Central Hospital
2024
University of California, Los Angeles
2017-2020
National Defense Medical College
2008-2018
National Defense Medical College Hospital
2008-2018
VA West Los Angeles Medical Center
2017
University of Shizuoka
2017
VA Greater Los Angeles Healthcare System
2017
Kobe Pharmaceutical University
2017
National Defence Medical Centre
2017
Tokyo Medical University
2000-2001
The presence of PCR inhibitors in water samples is well known and contributes to the fact that a practical assay has not been developed for legionella surveillance. In this study, we devised new seminested detection Legionella spp. as means overriding without loss sensitivity. utilized primers amplify 16S rRNA gene (LEG primers) 39 was specific legionellae, sensitivity 1 fg extracted DNA laboratory examination. To evaluate feasibility identifying it used survey contamination 49 cooling...
Lipopolysaccharides (LPS) are potent pro-inflammatory molecules that enter the systemic circulation from intestinal lumen by uncertain mechanisms. We investigated these mechanisms and effect of exogenous glucagon-like peptide-2 (GLP-2) on LPS transport in rodent small intestine. Transmucosal was measured Ussing-chambered rat jejunal mucosa. In anesthetized rats, appearance fluorescein isothiocyanate (FITC)-LPS into portal vein (PV) mesenteric lymph simultaneously monitored after...
We have found that Legionella dumoffii strain Tex-KL (ATCC 33343) invades into and proliferates in the human lung alveolar epithelial-cell line A549 vitro. The organism associated with cells at a 10-fold greater magnitude than L. pneumophila Philadelphia-1 during vitro coculture for 1 h. Thereafter, invaded significantly higher rate (100- to 1,000-fold) did Philadelphia-1. After internalization, however, both bacteria proliferated same rate. This finding led us examine bacterial localization...
Lymphatic failure is a histopathological feature of inflammatory bowel disease (IBD). Recent studies show that interaction between platelets and podoplanin on lymphatic endothelial cells (LECs) suppresses lymphangiogenesis. We aimed to investigate the role in process colitis, which likely be through modulation Lymphangiogenesis colonic mucosal specimens from patients with IBD was investigated by studying mRNA expression lymphangiogenic factors histologically examining vessel (LV) densities....
Liver fibrosis is a life-threatening disorder for which no approved therapy available. Recently, we reported that mouse hepatic stellate cell (HSC) activation increased free cholesterol (FC) accumulation, partly by enhancing signaling through sterol regulatory element-binding protein 2 (SREBP2) and microRNA-33a (miR-33a), resulted in HSC sensitization to transforming growth factor-β (TGFβ)-induced "vicious cycle" of liver fibrosis.Human HSCs were isolated from surgical specimens control...
In Crohn's disease (CD), assessment of activity and extension is important for clinical management. Endoscopy the most reliable tool evaluating in these patients it distinguishes between lesions based on ulcer, erosion, redness. Magnetic resonance imaging (MRI) less invasive than endoscopy; however, sensitivity MRI detecting believed to be lower, whether can detect milder has not been studied. The aim this study was compare detection ability magnetic enterocolonography (MREC) with...
Abstract Famciclovir is a guanine analog antiviral drug used commonly for herpes zoster. Efficacy of famciclovir treatment has been reported to be comparable valacyclovir treatment. Both these medications reduce the time complete cessation zoster‐associated pain including post‐herpetic neuralgia, as compared acyclovir. We conducted multicenter, randomized, open clinical trial in order evaluate extent relief afforded by two drugs during acute disease phase The study group comprised 86...
The enhanced recruitment of leukocytes to the inflamed colon is a key feature ulcerative colitis (UC). gut-specific adhesion molecules involved in leukocyte have emerged as recent therapeutic targets. Nicotine absorbed from smoking has been reported work protectively UC patients. Our hypothesis that nicotine may suppress aberrant and colonic inflammation via suppression overexpressed colon. To test this hypothesis, severity degree induced by were assessed dextran sulfate sodium (DSS) mice...
Uric acid is excreted from blood into the intestinal lumen, yet roles of uric in diseases remain to be elucidated. The study aimed determine whether could reduce end points associated with nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy.A mouse model NSAID-induced enteropathy was generated by administering indomethacin intraperitoneally 8-week-old male C57BL/6 mice, and then vehicle or administered orally. A group mice treated also concurrently inosinic acid, a precursor,...
Abstract To determine the infection source of a sporadic Legionella pneumonia case associated with hot spring bath, we used five molecular methods, including repetitive element polymerase chain reaction (rep‐PCR), arbitrarily primed PCR (AP‐PCR), ribotyping, restriction endonuclease analysis (REA), and macrorestriction (MREA) by pulsed‐field gel electrophoresis. L. pneumophila serogroup (SG) 3 strain EY 3702, isolated from an intratracheal specimen 71‐year‐old Japanese female who developed...
Serotonin (5-HT), predominantly synthesized and released by enterochromaffin cells, is implicated in gastrointestinal symptoms such as emesis, abdominal pain, diarrhea. Because luminal short-chain fatty acids (SCFAs) release 5-HT from which express the SCFA receptor free acid 2 (FFA2) rat duodenum, we examined effects of selective FFA2 agonist phenylacetamide-1 (PA1) on duodenal with consequent bicarbonate secretion [duodenal (DBS)] indomethacin (IND)-induced enteropathy. Intestinal injury...
Abstract Background and Aim Few drugs have been found satisfactory in the treatment of nonsteroidal anti‐inflammatory ( NSAIDs )‐induced enteropathy. T oll‐like receptor TLR ) 4 aberrant leukocyte migration to intestinal mucosa are reported be involved pathology enteropathy 2 agonists evoke hyposensitivity stimulation vitro. In this study, we investigated whether how lipoarabinomannan LAM or lipoteichoic acid LTA ), agonists, attenuated indomethacin IND damage. Methods (0.5 mg/kg) (15 was...
We present the case of a 25-year-old woman at 16 weeks gestation who presented with non-comatose autoimmune acute liver failure and was high risk developing fulminant hepatitis. Predictive formulas indicated probability Unenhanced computed tomography showed marked hepatic atrophy broadly heterogeneous hypoattenuating areas. The course her illness subacute, etiology injury unclear. Considering all above, we predicted poor prognosis. Plasma exchange (PE) continuous hemodiafiltration (CHDF)...
Xenin-25, a neurotensin (NT)-related anorexigenic gut hormone generated mostly in the duodenal mucosa, is believed to increase rate of ion secretion, because xenin-induced diarrhea not present after Roux-en-Y gastric bypass surgery. Because local effects xenin on secretion have remained uninvestigated, we thus examined neural pathways underlying anion secretion. Intravenous infusion xenin-8, bioactive C-terminal fragment xenin-25, dose dependently increased HCO<sub>3</sub><sup>−</sup>...
Recent studies suggest that primary low-grade gastric lymphomas of mucosa-associated lymphoid tissue (MALT) are cured in many cases between 1 and 18 months after H. pylori eradication. The aim this study is to elucidate when complete regression (CR) MALT lymphoma can be histologically predicted eradication.Twenty-one patients with were treated triple therapy (amoxicillin, clarythromycin proton pump inhibitor) for 14 days. Subsequently, they followed up by sequential endoscopy biopsy (number...
We examined the intracellular growth of 20 strains within six species Legionella in thioglycolate-elicited peritoneal macrophages from A/J and C57BL/6 mice a congenic strain derived them (A.B Lgn1). With exception pneumophila Togus-1 Bloomington-2, 15 L. tested was controlled by Lgn1. However, five other than not under Lgn1's control.
Phlebosclerotic colitis is a rare and recently known disease entity its etiology still to be elucidated. Some phlebosclerotic cases are difficult distinguish from collagenous because of the similarity pathological findings. In all J apanese case reports in which an association with use C hinese herbal medicine suspected, sansisi (gardenia fruit) was included, suggesting pathogenesis this disease. We report that wasdifficult distinguished colitis, suspected as cause
Thrombomodulin (TM) is an anticoagulant cofactor protein. We hypothesized that its recombinant soluble TM (rhTM) form, widely used to treat disseminated intravascular coagulation, might have anti-inflammatory action in inflammatory bowel disease (IBD), possibly through inhibition of high-mobility group box 1 protein (HMGB1).We investigated effects HMGB1 and effect rhTM dextran sulfate sodium (DSS)-treated mice, some cell lines ulcerative colitis (UC) patients, particularly focusing on...
Sphingosine-1-phosphate (S1P) receptor 1, a therapeutic target of the S1P
Abstract We examined the effects of temperature on interaction between Legionella pneumophila and phagocytes guinea pigs. The body temperatures pigs infected with a sublethal dose (1.2 × 10 4 CFU) or lethal (1.0 5 L. elevated from 38.4±0.15 C to 40.2±0.42 40.3 ± 0.62 C, respectively. intracellular bacterial killing by proliferation in were at 33, 37, 40, 42 using vitro culture systems peritoneal macrophages polymorphonuclear leukocytes (PMN) In all incubated different temperatures,...