The malignant phenotype of tumour cells is fuelled by changes in the expression various transcription factors, including some well-studied proteins such as p53 and Myc. Despite significant progress made, little known about several other ELF4, how they help shape oncogenic processes cancer cells. To this end, we performed a bioinformatics analysis to facilitate detailed understanding variations ELF4 human cancers are related disease outcomes cell drug responses. Here, using mRNA data 9,350...

Introduction: Epstein-Barr virus (EBV) is a ubiquitous that infects more than 90% of the world's population, and implicated in lymphoma pathogenesis. However, Zambia during diagnosis these lymphomas, association with lymphomas not established. Since most patients have poor prognosis, identification within lesion will allow for targeted therapy. The aim this study was to provide evidence presence EBV diagnosed at University Teaching Hospital (UTH) Lusaka, Zambia.

Investigating the human genome is vital for identifying risk factors and devising effective therapies to combat genetic disorders cancer. Despite extensive knowledge of "light genome", poorly understood "dark genome" remains understudied. In this study, we integrated data from 20,412 protein-coding genes in Pharos 8,395 patient-derived tumours The Cancer Genome Atlas (TCGA) examine pharmacological dependencies cancers their treatment implications. We discovered that dark exhibited high...

Pancreatic cancer remains a significant public health problem with an ever-rising incidence of disease. Cancers the pancreas are characterised by various molecular aberrations, including changes in proteomics and genomics landscape tumour cells. Therefore, there is need to identify proteomic pancreatic specific genomic alterations associated disease subtypes. Here, we carry out integrative bioinformatics analysis The Cancer Genome Atlas dataset, whole-exome sequencing data collected from...

The diagnosis and evaluation of impaired renal function remains a challenge owing to lack reliable biomarker for assessment kidney function. existing panel biomarkers currently displays several limitations, recently injury molecule-1 (KIM-1) has been suggested as sensitive proposed enter clinical practice.This study was conducted determine the diagnostic value serum creatinine, urea, microalbuminuria (MAU) in relation novel biomarker, KIM-1.Serum MAU, KIM-1 were measured forty individuals...

Sickle cell disease is a group of hemoglobin (Hb) disorders resulting from the inheritance sickle β-globin gene. It most common pathological Hb mutation worldwide with 75% being born in Sub-Saharan Africa.This study aims to determine if dried blood spots (DBSs) can be used for diagnosis newborns. In Zambia, there no neonatal screening program anemia (SCA), yet it has been proved that early by newborn (NBS) using DBSs and access comprehensive care results survival adulthood over 96%...

Abstract Since the earliest reports of Coronavirus disease - 2019 (COVID-19) in Wuhan, China December 2019, has rapidly spread worldwide, attaining pandemic levels early March 2020. However, COVID-19 differed African setting compared to countries on other continents. To predict Africa and within each country continent, we applied a Susceptible-Infectious-Recovered mathematical model. Here, our results show that, overall, is currently (July 24, 2020) at peak pandemic, after which number cases...

Abstract Investigating the human genome is vital for identifying risk factors and devising effective therapies to combat genetic disorders cancer. Despite extensive knowledge of “light genome”, poorly understood “dark genome” remains understudied. In this study, we integrated data from 20,412 protein-coding genes in Pharos 8,395 patient-derived tumours The Cancer Genome Atlas (TCGA) examine pharmacological dependencies cancers their treatment implications. We discovered that dark exhibited...

Abstract Cervical cancer has remained the most prevalent and lethal malignancy among women worldwide accounted for over 250,000 deaths in 2019. Nearly ninety-five per cent of cervical cases are associated with persistent infection high-risk Human Papillomavirus (HPV), seventy these viral integration host genome. HPV-infection imparts specific changes regulatory network infected cells that diagnostic, prognostic importance. Here, we conducted a systems-level analysis changes, proteins...

Human herpes virus-8, a γ2-herpes virus, is the aetiological agent of Kaposi sarcoma. Recently, Kaposi's sarcoma cases have increased in Zambia. However, diagnosis this disease based on morphological appearance affected tissues using histological techniques, and association with its causative agent, Herpes virus 8 not sought. This means poor prognosis for patients since targeted during KS lesions may be mistaken other reactive neoplastic vascular proliferations when only techniques are used....

Abstract Pancreatic cancer remains a significant public health problem with an ever-rising incidence of disease. Cancers the pancreas are characterised by various molecular aberrations, including changes in proteomics and genomics landscape tumour cells. There is need, therefore, to identify proteomic pancreatic specific genomic alterations associated disease subtypes. Here, we carry out integrative bioinformatics analysis The Cancer Genome Atlas dataset that includes whole-exome sequencing...

Abstract In recent years, there has been growing interest in understanding the role of dark genes genetic diseases and phenotypes. Despite their lack functional characterisation, account for a significant portion human genome are believed to play regulating gene expression cellular processes. We investigated phenotypes by conducting integrative network analyses enrichment studies across multiple large-scale molecular datasets. Our investigation revealed predominant association both light...

Since the earliest reports of Coronavirus disease - 2019 (COVID-19) in Wuhan, China December 2019, has rapidly spread worldwide, attaining pandemic levels early March 2020. However, COVID-19 differed African setting compared to countries on other continents. To predict Africa and within each country continent, we applied a Susceptible-Infectious-Recovered mathematical model. Here, our results show that, overall, is currently (May 29, 2020) at peak pandemic, after which number cases would...

The malignant phenotype of tumour cells is fuelled by changes in the expression various transcription factors, which include some well-studied proteins such as p53 and Myc. Despite significant progress made, little known about several other including ELF4, how they help shape oncogenic processes that occur cancer cells. To this end, we performed a bioinformatics analysis to facilitate detailed understanding transcriptional variations ELF4 human cancers are related disease outcomes cell drug...

Cervical cancer has remained the most prevalent and lethal malignancy among women worldwide accounted for over 250,000 deaths in 2019. Nearly ninety-five per cent of cervical cases are associated with persistent infection high-risk Human Papillomavirus (HPV), seventy these viral integration host genome. HPV-infection imparts specific changes regulatory network infected cells that diagnostic, prognostic importance. Here, we conducted a systems-level analysis changes, proteins thereof,...

Abstract Acute myeloid leukaemia (AML) is a heterogeneous disease with complex pathogenesis that affects hematopoietic stem cells. Ethnic and racial disparities have been reported to affect treatment survival outcomes in AML patients. Here, we analysed clinical transcriptomic data from The Cancer Genome Atlas (TCGA) investigate potential differences the genetic landscape of between African European individuals. We found several differentially expressed mRNA transcripts Africans Europeans....

Abstract Anaemia is a condition in which either the number of red blood cells or their oxygen-carrying capacity insufficient to meet physiologic needs, vary by age, sex, altitude, smoking and pregnancy status. The global estimate childhood anaemia indicates that 293.1 million children are anaemic, 28.5% these reside sub-Sahara Africa. Also, significant public health problem with high age-standardised death rate 11.18 per 100,000 Zambia. We conducted cross-sectional study involving 392...