A5071549825- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
- ATP Synthase and ATPases Research
- T-cell and B-cell Immunology
- Autophagy in Disease and Therapy
- Cancer, Hypoxia, and Metabolism
- Single-cell and spatial transcriptomics
- Drug Transport and Resistance Mechanisms
- Photodynamic Therapy Research Studies
- Immunotherapy and Immune Responses
- melanin and skin pigmentation
- Nanoplatforms for cancer theranostics
- Probiotics and Fermented Foods
- Listeria monocytogenes in Food Safety
- Retinoids in leukemia and cellular processes
- Molecular Biology Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Adipose Tissue and Metabolism
- Skin Protection and Aging
- Microbial infections and disease research
- Photosynthetic Processes and Mechanisms
- Cancer therapeutics and mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Response and Inflammation
- Cell Image Analysis Techniques
Université de Limoges
1991-2017
CNR de la Résistance aux Antibiotiques
2011
Zero to Three
2007
Centre National de la Recherche Scientifique
1999-2003
Contrôle de la Réponse Immune B et Lymphoproliférations
2003
Christian Dior (France)
1997
Technologie Médicale (France)
1993
Centre Hospitalier Universitaire de Limoges
1988-1990
The acridine orange derivative, 10 N ‐nonyl orange, is an appropriate marker of the inner mitochondrial membrane in whole cells. We use model systems to demonstrate that binds negatively charged phospholipids (cardiolipin, phosphatidylinositol and phosphatidylserine). stoichiometry has been found be 2 mol orange/mol cardiolipin 1 dye/mol phosphatidylserine or phosphatidylinositol, while, with zwitterionic phospholipids, significant binding could not detected. affinity constants were × 6 M −1...
The distribution of cardiolipin across the inner mitochondrial membrane was directly determined by using ability fluorescent dye 10‐ N ‐nonyl‐3,6‐bis(dimethylamino)acridine (10‐ ‐nonyl acridine orange) to form dimers when it interacts with diacidic phospholipid. Two independent methods were employed: (a) a spectrophotometric measurement orange binding isolated rat liver mitochondria, mitoplasts and inside‐out submitochondrial particles, (b) flow‐cytometric analysis specific red fluorescence,...
Abstract: Studies have established that autophagy constitutes an efficient process to recycle cellular components and certain proteins. The phenomenon was demonstrated primarily in response nutrient starvation, there are increasing evidences it is implied differentiation. Keratinocyte differentiation going along activation of lysosomal enzymes organelle clearance, terminal steps sometimes described as a specialized form cell death leading corneocytes. We examined whether initiation the human...
Abstract Due to its spectral characteristics, the fluorochrome nonyl acridine orange (NAO) (λ abs :489 nm, λ em :525 nm), which is spontaneously incorporated by mitochondria with a high relative specificity, provides new probe for in situ study of these organelles flow cytometry. In 15 min at 20°C, dye 4.75 × 10 −6 M saturates mitochondrial binding sites present 1.5 6 cells. Unlike Rh 123, fixation not affected action uncouplers and ionophores. orange, sensitive nucleases. By studying...
Mesentericin Y105, a bacteriocin produced by Leuconostoc mesenteroides strain, dissipates the plasma membrane potential of Listeria monocytogenes and inhibits transport leucine glutamic acid. It also induces an efflux preaccumulated amino acids from cells. In addition, uncouples mitochondria increasing state 4 respiration decreasing 3 respiration. The ATP synthase adenine nucleotide translocase organelle while affinity ADP for its carrier is not modified. results suggest that mesentericin...
Abstract MTT reduction is usually analysed by colorimetric assay to study mitochondrial dehydrogenase activity as a test of cytotoxicity. This enzymatic reaction produces dark‐blue granules formazan, which increase cell refringency. In this work, we define the conditions for use in quantitative flow cytometric analysis. provides nonfluorescent dye usable technique an intracellular NADH‐dependent vital cells. We observe that formazan production increases asymptotically with concentration and...
Cobalt chloride (CoCl2), a hypoxia-mimetic agent, induces reactive oxygen species (ROS) generation, leading to cell death. Divergent data have been reported concerning p53 implication in this apoptotic mechanism. In study, we studied cobalt-induced death neuroblastoma lines carrying wild-type (WT) ( SHSY5Y) and mutated DNA-binding domain [SKNBE(2c)]. CoCl2 induced an upregulation of p53, p21 PUMA expression WT cells but not SKNBE(2c). SHSY5Y cells, serine-15 phosphorylation appeared early (6...
Apoptosis is currently studied by flow cytometry with mitochondrial membrane potential (Deltapsimt) and integrity fluorochromes. Rhodamine 123 DiOC6(3) remain controversial to identify cells displaying a low Deltapsimt. JC-1 constitutes good Deltapsimt indicator, due fluorescence shift from green orange emission, according the increase in Nevertheless, it not feasible analyze simultaneously propidium iodide. Among available fluorescent probes, TOTO-3 seems be candidate for double staining...
Flow cytometry can measure variations in DNA content and chromatin structure as well dramatic changes the mitochondria of germ cells during maturation from spermatogonia to elongated spermatids. Using 10-N nonyl acridine orange (NAO), an inner mitochondrial membrane dye, it is easy follow rearrangements. Mouse testis stained with fluorescent probe propidium iodide (PI) analyzed by flow be discriminated on basis their ploidy levels into five main regions corresponding spermatids, round...
Glioblastoma multiform (GBM), the most common and aggressive primary brain tumor, is characterized by a high degree of hypoxia resistance to therapy because its adaptation capacities, including autophagy growth factors signaling. In this study, we show an efficient hypoxia-induced survival in four different GBM cell lines (U87MG, M059K, M059J LN-18) activation particular neurotrophin signaling pathway. Indeed, enhancement both TrkC NT-3 was followed downstream p38MAPK phosphorylation,...