A5081175565- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- PARP inhibition in cancer therapy
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Radioactivity and Radon Measurements
- Carcinogens and Genotoxicity Assessment
- Radioactive contamination and transfer
- Acute Myeloid Leukemia Research
- Nuclear and radioactivity studies
Novo Nordisk Foundation
2023
University of Copenhagen
2023
Universidad de Las Palmas de Gran Canaria
2022
Universidad de La Laguna
2019-2020
Hospital Universitario de Canarias
2014-2020
Chk1, an essential checkpoint kinase in the DNA damage response pathway (DDR), is tightly regulated by both ATR-dependent phosphorylation and proteasome-mediated degradation. Here we identify ubiquitin hydrolase USP7 as a novel regulator of Chk1 protein stability. was shown before to regulate other DDR proteins such p53, Hdm2 Claspin, adaptor ATR-Chk1 required for activation. Depletion or inhibition leads lower levels. The decreased after knock down cannot be rescued simultaneously elevating...
Abstract Post-translational histone modifications and chromatin remodelling play a critical role controlling the integrity of genome. Here, we identify lysine demethylase PHF2 as novel regulator DNA damage response by regulating damage-induced focus formation 53BP1 BRCA1, factors in pathway choice for double strand break repair. knockdown leads to impaired BRCA1 delays resolution foci. Moreover, irradiation-induced RPA phosphorylation formation, well localization CtIP, required end...
Abstract The cellular response to DNA breaks is influenced by chromatin compaction. To identify regulators involved in the damage response, we screened for genes that affect recovery following using an RNA i library of regulators. We identified remodeling, sister chromatid cohesion, and histone acetylation not previously associated with checkpoint recovery. Among these PHD finger protein 6 ( PHF 6), a gene mutated Börjeson–Forssman–Lehmann syndrome leukemic cancers. find loss dramatically...
Abstract SUMOylation regulates numerous cellular processes, but what represents the essential functions of this protein modification remains unclear. To address this, we performed genome-scale CRISPR–Cas9-based screens, revealing that BLM-TOP3A-RMI1-RMI2 (BTRR)-PICH pathway, which resolves ultrafine anaphase DNA bridges (UFBs) arising from catenated structures, and poorly characterized NIP45/NFATC2IP become indispensable for cell proliferation when is inhibited. We demonstrate NIP45...
ABSTRACT Post-translational histone modifications and chromatin remodelling play a critical role in the mechanisms controlling integrity of genome. Here we identify lysine demethylase PHF2 as novel regulator DNA damage response by regulating balance between damage-induced focus formation 53BP1 BRCA1, factors pathway choice for double strand break repair. knock down leads to impaired BRCA1 delays resolution foci. Moreover, irradiation-induced RPA phosphorylation formation, well localization...