A5016888268- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Immunotherapy and Immune Responses
- Infectious Diseases and Mycology
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Vibrio bacteria research studies
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Gene expression and cancer classification
- Microbial Metabolism and Applications
- Animal Genetics and Reproduction
- Bacteriophages and microbial interactions
- Parasite Biology and Host Interactions
- Genomics and Phylogenetic Studies
- Bioactive Compounds and Antitumor Agents
- vaccines and immunoinformatics approaches
- Genomic variations and chromosomal abnormalities
- Fish Ecology and Management Studies
University of Iceland
2016-2018
Institut Scientifique de Santé Publique
2011-2014
Reykjavík University
2013
National University Hospital of Iceland
2013
University of Pittsburgh
2008-2012
National Institute of Infectious Diseases
2004
Osaka University
2002
After short duplexes of synthetic 21-23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector-based approach for siRNAs was demonstrated mammalian cultured cell lines. However, the effect RNA interference (RNAi) on various differentiated cells live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein every part mouse and rat body. These results suggest...
The host immune response is generally sufficient to contain Mycobacterium tuberculosis infection. It does not, however, efficiently prevent subsequent infection with M. or provide sterilizing immunity. While the understanding of generated against this pathogen incomplete, improvements have been achieved due advances in immunological tools. In study, we analyzed multifunctional nature primary and memory CD8 T-cell responses during murine We a recombinant strain expressing ovalbumin (OVA)...
Abstract Amplification of 8p12‐p11 is relatively common in breast cancer and several genes within the region have been suggested to affect tumor progression. The aim study was map amplified a large set tumors an effort identify genetic driver explore its impact on progression prognosis. Copy number alterations ( CNA s) were mapped 359 tumors, gene expression data from 577 (359 included) correlated with , clinical–pathological factors, protein (39 tumors). 11.4% tumors. smallest amplification...
There is no effective vaccine against Buruli ulcer. In experimental footpad infection of C57BL/6 mice with M. ulcerans, a prime-boost vaccination protocol using plasmid DNA encoding mycolyltransferase Ag85A ulcerans and homologous protein boost has shown significant, albeit transient protection, comparable to the one induced by bovis BCG. The mycolactone toxin an obvious candidate for vaccine, but virtue its chemical structure, this not immunogenic in itself. However, antibodies some...
Abstract Winter ulcer disease, caused by Moritella viscosa , is a significant problem in cold water salmonid farming, although the bacterium can infect and cause disease number of other fish species, such as lumpfish ( Cyclopterus lumpus ). Lumpfish are used cleaner fish, to eat sea lice from Atlantic salmon Salmo salar ) pens. It remains be established whether M. transmitted between species. In this study, we examined isolate could lumpfish. We further salmon. Finally, vaccination with...
Pasteurella multocida can be part of the upper respiratory flora animals, but under conditions stress or immunocompromisation, bacteria cause severe symptoms. In this study, we compared 10 P. isolates from Icelandic sheep with symptoms and 19 apparently healthy abattoir sheep. We examined capsule type, genetic variability presence toxA gene in two groups. Surprisingly, found that all ovine study carried gene, which markedly differs what has been published other studies. Interestingly,...
Mycoplasma ovipneumoniae is a pathogen that causes atypical pneumoniae in sheep and goats. While infection of lambs can induce strong immune responses, typically measured as serum antibodies, experimental vaccines appear to lower antibody titres. The purpose this study was better understand the bacterium its interaction with host, order improve vaccination strategy.We designed primers compare seven M. gene sequences, addition 16S sequence used, estimate variability between isolates. In...
Transgenes can affect transgenic mice via transgene expression or the so-called positional effect. DNA sequences be localized in chromosomes using recently established mouse genomic databases. In this study, we describe a chromosomal mapping method that uses walking technique to analyze flank transgenes, combination with genome database searches. Genomic was collected from two lines harboring pCAGGS-based and adaptor-ligated, enzyme restricted libraries for each line were constructed....
Flavobacterium species cause significant disease in salmonid farming worldwide, typically seen as mortality sac fry and later necrosis ulceration fingerlings fry. In this study, we sampled Atlantic salmon Salmo salar Arctic char Salvelinus alpinus from 5 Icelandic fish farms 2014 2017, where flavobacteria were suspected to disease. The objective of the study was identify characterise bacteria by sequencing 16S rRNA gene multilocus sequence type housekeeping genes. We found distinct groups...
Abstract M. tuberculosis (Mtb) infection in mice results a slow but steady increase bacterial numbers the lungs, peaking at 3-4 weeks, followed by state of chronic with slightly lower than peak. While T cells have been shown to be important for containing Mtb infection, only known method clearing bacteria is extended antibiotic treatment. Our previous showed that Mtb-specific CD8 were limited either cytotoxicity or secretion IFN-g, indicating functional defect cells. The majority cytotoxic,...
Abstract Mycobacterium tuberculosis (Mtb), causing (TB), is estimated to infect one third of the human population, and kills about 1.8 million people per year. CD8 T cells are essential components in adaptive immunity against Mtb. We previously demonstrated that Mtb-specific cell populations divided into two distinct types: either cytotoxic or IFN-γ secreting. However, cytokine signals prime evolve these phenotypes, develop capability have both producing functions not known. found naïve...
The immune response against Mycobacterium tuberculosis (Mtb) is generally sufficient to contain, but not clear, the infection. Memory forms following Mtb infection and BCG vaccination, doesn't protect from reinfection with has limited effect on bacterial load. Our research aimed at better understanding CD8 T cell responses identify host factors that prevent clearing of bacteria. We created recombinant expressing OT1 OT2 epitopes, recognized by CD4 cells respectively, which allows us follow...