We demonstrate a handheld on-chip biosensing technology that employs plasmonic microarrays coupled with lens-free computational imaging system towards multiplexed and high-throughput screening of biomolecular interactions for point-of-care applications resource-limited settings. This lightweight field-portable device, weighing 60 g 7.5 cm tall, utilizes compact optoelectronic sensor array to record the diffraction patterns nanostructures under uniform illumination by single-light emitting...

Plasmonic nanohole arrays have received significant attention, as they highly advantageous optical properties for ultrasensitive and label-free biosensing applications. Currently, most of these subwavelength periodic apertures are mainly implemented on transparent materials, which results in multiple spectrally close transmission resonances. However, this spectral characteristic is not ideal applications, it complicates monitoring variations. In article, utilizing a hybrid substrate composed...

We demonstrate a high-throughput biosensing device that utilizes microfluidics based plasmonic microarrays incorporated with dual-color on-chip imaging toward real-time and label-free monitoring of biomolecular interactions over wide field-of-view >20 mm2. Weighing 40 grams 8.8 cm in height, this biosensor an opto-electronic imager chip to record the diffraction patterns nanoapertures embedded within microfluidic channels, enabling analyte exchange. This is simultaneously illuminated by two...

This article reports the results of a curriculum survey 322 analytical chemistry instructors (92% U.S.-based) conducted during Spring 2021. snapshot current course formats, topics, and pedagogical methods will be useful to both seasoned novice teachers chemistry. The majority respondents reported that their major requires two courses, 97% at least one is required. Over 80% required lab course. topic rated most important was standardization calibration methods, followed by UV–vis absorbance...

A two-dimensional array of gold nanotriangles inscribed onto glass coverslips were optimized for the surface-enhanced Raman detection streptavidin/biotin monolayer assemblies. The nanostructures fabricated by electron beam lithography, and its optical parameters to be probed under a microscope with linearly polarized He−Ne laser an excitation wavelength λ = 632.8 nm. platforms first tested against biotinylated alkanethiols (BAT) functionalized over nanostructure, showing that good-quality...

Metallic nanotriangles arranged in a hexagonal lattice are common nanostructures used for surface-enhanced Raman spectroscopy. Such structures, generally made by nanosphere lithography or electron beam (EBL), exhibit localized surface plasmon resonance (LSPR) at the junctions between two facing nanotriangles. Localized and efficient enhancement of electromagnetic field is expected when light with matching LSPR frequency impinging sample. Herein, first, we report modeling both gold silver...

Effective analyte delivery is essential to achieve rapid and sensitive biodetection systems. In this article, we present an actively controlled fluidic system integrated with a suspended plasmonic nanohole sensor superior efficiency ultrafast response, as compared conventional 70 nm sized virus like solution used experimentally demonstrate the performance improvements. Sensor response time reduced by one order of magnitude methods. A seven orders dynamic concentration range from 103 109...

Osteopontin (OPN) inhibits the nucleation and/or growth of several biominerals, including hydroxyapatite (HA) and calcium oxalate monohydrate (COM), is thought to function in prevention soft-tissue calcification. In previous studies, pOPAR, a peptide corresponding amino acids 65–80 rat bone OPN (pSHDHMDDDDDDDDDGD), was shown be potent inhibitor HA crystal growth. We now show that formation COM presence this results plate-shaped crystals with rounded ends scalloped {100} faces. Measurement...

Nanomaterials use in traditional spectroscopic assays has enabled the development of more sensitive and accessible methods with wider-range applications. Usually, sensitivity nanosensors is improved through incorporation labels or by inducing nanoparticles aggregation. Herein, we present a label-free, selective, reliable, affordable gold nanoprisms aptasensor to detect content ochratoxin A (OTA) suspension surface-enhanced Raman spectroscopy (SERS). Label-free SERS provides molecular...

We report the fabrication and optical study of Fisher's patterns inscribed on glass slides. Such structures, fabricated by electron beam lithography, consist gold nanotriangles, organized in a hexagonal arrangement. By changing conditions, it is possible to control precisely size structures gap distance between facing triangles but most importantly, finely tune their localized surface plasmon resonance. In addition experimental studies, plasmonic properties Fischer's were characterized as...

In this paper, we demonstrate a novel method for high throughput patterning of bioprobes with nanoscale features on biocompatible polymer substrate. Our technique, based nanostencil lithography, employs resolution and robust masks integrated array reservoirs. We show that the smallest pattern size can reach down to 100 nm. also different types biomolecules be patterned same substrate simultaneously. Furthermore, stencil reused multiple times generate series identical patterns at low cost....

The electric field interaction of hexagonal arrays gold nanotriangles positioned on glass slides was studied using finite-difference time domain (FDTD) simulations. length the triangles (L = 100−300 nm), irradiation wavelength (λ 400−1100 and direction linearly polarized input (either Px or Py) were three variables considered in calculation spectra localized surface plasmons. results show that plasmonic properties this array nanostructures are susceptible to modification by changing any...

Skin wound healing is a complex biological process of tissue regeneration in which the dressing crucial for rapid healing; it must protect keep an adequate level moisture and prevent infections. Alginate (AL), polysaccharide from brown algae, has been extensively studied treatment, aloe vera gels (AVGs) have also used treatment skin. The AVG main bioactive was combined with AL preparation membranes. Two-dimensional membranes were prepared by casting and, comparison, transparent nanoparticle...

We introduce a hand-held biosensor based on large-area plasmonic microarrays coupled with lensfree computational imaging system for high-throughput biosensing. Our light-weight device, 60 g and 7.5 cm, is highly suitable point-of-care applications field-settings.

We report the development of a peptide-based optical nanoprobe specifically tailored for prostate cancer imaging. The imaging probe is comprised cyclic peptide nanotubes, formed

The potential bactericidal properties of nanosized zinc oxide (ZnO) make this semiconductor suitable for medical and pharmaceutical applications.In article we present two methods synthesis ZnO nanoparticles (Nps-ZnO): chemical precipitation solution combustion synthesis.The synthesized nanopowders were characterized with Scanning Electron Microscopy-Energy Dispersive X-Ray Spectroscopy (SEM-EDS), X-ray Diffraction (XRD) Transmission Microscopy (TEM).The EDS analysis indicate a Zn:O atomic...