A5063427107- CRISPR and Genetic Engineering
- Neuropeptides and Animal Physiology
- Genetics, Aging, and Longevity in Model Organisms
- Innovation and Socioeconomic Development
- Neuroendocrine regulation and behavior
- Reproductive biology and impacts on aquatic species
- Neurobiology and Insect Physiology Research
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Developmental Biology and Gene Regulation
- Hypothalamic control of reproductive hormones
- Chromosomal and Genetic Variations
University of Hong Kong
2022-2024
Xiamen University
2020-2024
The cephalochordate amphioxus is a promising animal model for studying the origin of vertebrates due to its key phylogenetic position among chordates. Although transcription activator-like effector nucleases (TALENs) have been adopted in genome editing, labor-intensive construction TALEN proteins limits usage many laboratories. Here we reported an application CRISPR/Cas9 system, more amenable editing method, this group animals. Our data showed that while co-injection Cas9 mRNAs and sgRNAs...
The Hatschek's pit in the cephalochordate amphioxus, an invertebrate deuterostome basal to chordates is suggested be functional homolog structure of vertebrate adenohypophysis based on anatomy and expression homologous neuroendocrine genes. However, endocrine potential remains demonstrated as well physiological actions secreted neuropeptides. In this study, we have explored distribution characterize function amphioxus PACAP/GCG precursor, which ortholog hypothalamic PACAP neuropeptide...
Abstract Sex determination is remarkably variable among animals with examples of environmental sex determination, male heterogametic (XX/XY) and female (ZZ/ZW) chromosomal other genetic mechanisms. The cephalochordate amphioxus occupies a key phylogenetic position as basal chordate outgroup to vertebrates, but its mechanism unknown. During the course generating Nodal mutants transcription activator-like effector nucleases (TALENs) in Branchiostoma floridae, serendipitously, we generated...
Amphioxus is a promising model organism for understanding the origin and evolution of vertebrates due to its basal phylogenetic position among chordates. We here compared mutation efficacy type tail tips gametes amphioxus founders injected with Cas9 protein six different sgRNAs targeting five distinct genes, revealed strong correlation mild two tissues. In addition, we also observed positive relationship between gene insertions in Tol2 transposase transgenic constructs. Finally, showed that...
Abstract The cephalochordate amphioxus is a promising animal model for studying the origin of vertebrate due to its key phylogenetic position among chordates. Although transcription activator-like effector nucleases (TALENs) has been adopted in genome editing, labor intensive construction TALEN proteins limits usage many laboratories. We here report an application CRISPR/Cas9 system, more amenable editing method, this group animals. Our data show that while co-injection Cas9 mRNAs and sgRNAs...