A5009889196- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- Cellular Mechanics and Interactions
- Neurofibromatosis and Schwannoma Cases
- Wnt/β-catenin signaling in development and cancer
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Axon Guidance and Neuronal Signaling
- Invertebrate Immune Response Mechanisms
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Signaling Pathways in Disease
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Bioinformatics and Genomic Networks
- Viral Infectious Diseases and Gene Expression in Insects
- Silk-based biomaterials and applications
- Cell death mechanisms and regulation
- Click Chemistry and Applications
- Protist diversity and phylogeny
- Marine Invertebrate Physiology and Ecology
- Advanced Fluorescence Microscopy Techniques
- Vascular Malformations Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
University of Chicago
2013-2024
Marine Biological Laboratory
2020
Institute of Molecular and Cell Biology
2019
Duke University
1993-2005
House Clinic
2000
Yale University
1989-1993
Howard Hughes Medical Institute
1990-1993
Seattle University
1985
University of Washington
1985
Genetic analyses have raised the possibility of interactions between gene products neurogenic loci Notch and Delta, each which encodes a transmembrane protein with EGF homology. To examine intermolecular association these two genes, we studied effects their expression on aggregation in Drosophila S2 cells. We find that Notch-expressing cells form mixed aggregates specifically express Delta this process is calcium dependent. In addition, show can associate within membrane single cell,...
The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based technology to screen 102 bait proteins from melanogaster , most them orthologous cancer-related and/or signaling proteins, against high-complexity fly cDNA libraries. More than 2300 protein-protein interactions (PPI) were identified, which 710 are high confidence. computation reliability score for...
ABSTRACT Protein 4.1 functions to link transmembrane proteins with the underlying spectrin/actin cytoskeleton. To permit a genetic analysis of developmental role and cellular this membrane-skeletal protein, we have identified characterized its Drosophila homologue (termed D4.1). D4.1 is localized septate junctions epithelial cells encoded by coracle gene, new locus whose primary mutant phenotype failure in dorsal closure. In addition, mutations dominantly suppress Ellipse, hypermorphic...
The Notch gene in Drosophila encodes a transmembrane protein with homology to EGF that appears mediate cell-cell interactions necessary for proper epidermal vs. neural fate decisions. In this study, we examine expression detail throughout embryonic and imaginal development using confocal laser-scanning microscopy specific mAb probes. We find is expressed tissue-specific manner as early the cellular blastoderm stage, when cells of presumptive mesoderm clearly express less than adjacent...
One essential function of epithelia is to form a barrier between the apical and basolateral surfaces epithelium. In vertebrate epithelia, tight junction primary paracellular flow across whereas in invertebrate septate (SJ) provides this function. study, we identify new proteins that are required for functional Drosophila. addition previously known components Coracle (COR) Neurexin (NRX), show four other proteins, Gliotactin, Neuroglian (NRG), both α β subunits Na+/K+ ATPase, formation...
ABSTRACT Delta and Notch function are required for cell fate specification in numerous tissues during embryonic postembryonic Drosophila development. is expressed by all members of interacting populations within which fates being specified subsequently down-regulated as cells stably adopt particular fates. Multiphasic expression the derivatives many germ layers implies successive requirements a number tissues. At cellular level, generally coincident developing subcellular localized apparent...
Although extensively studied biochemically, members of the Protein 4.1 superfamily have not been as well characterized genetically. Studies coracle, a DrosophilaProtein homologue, provide an opportunity to examine genetic functions this gene family. coracle was originally identified dominant suppressor ofEgfr Elp , hypermorphic form theDrosophila Epidermal growth factor receptor gene. In article, we present phenotypic analysis ofcoracle, one first for member superfamily. Screens new alleles...
ABSTRACT The Notch gene in Drosophila encodes a transmembrane protein with homology to EGF that, variety of tissues, appears mediate cell interactions necessary for fate choices. Here we demonstrate that oogenesis and spermatogenesis depend on Notch. We examine the phenotypes temperature-sensitive allele, Nts1, and, using monoclonal antibody, determine cellular subcellular distribution during oogenesis. show Nts1 is associated missense mutation extracellular, homologous region at...
ABSTRACT Neurofibromatosis-2 is an inherited disorder characterized by the development of benign schwannomas and other Schwann-cell-derived tumors associated with central nervous system. The tumor suppressor gene encodes Merlin, a member Protein 4.1 superfamily most closely related to Ezrin, Radixin Moesin. This discovery suggested novel function for family members in regulation cell proliferation; proteins this were previously thought primarily link transmembrane underlying cortical actin....
Interest in members of the protein 4.1 super-family, which includes ezrin-radixin-moesin (ERM) group, has been stimulated recently by discovery that human neurofibromatosis 2 (NF2) tumor suppressor gene encodes an ERM-like protein, merlin. Although many proteins this family are thought to act linking actin-based cytoskeleton transmembrane proteins, cellular functions merlin have not defined. To investigate and developmental these we identified characterized Drosophila homologues moesin...
The Notch locus of Drosophila melanogaster is one a small number zygotically acting "neurogenic" genes involved in the correct segregation neural from epidermal lineages during embryogenesis as well other postembryonic developmental events. We have generated antibody probes against three regions protein to study expression and begin biochemical characterization protein. Consistent with predictions based on DNA sequence data, here we gather evidence showing that encodes large, glycosylated...
Merlin, the product of Neurofibromatosis type 2 (NF2) tumor-suppressor gene, is a member protein 4.1 superfamily that most closely related to ezrin, radixin, and moesin (ERM). NF2 dominantly inherited disease characterized by formation bilateral acoustic schwannomas other benign tumors associated with central nervous system. To understand its cellular functions, we are studying Merlin homologue in Drosophila. As case for tumors, Drosophila cells lacking function overproliferate relative...