A5077409122- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Diphtheria, Corynebacterium, and Tetanus
- Antibiotic Resistance in Bacteria
- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- Pneumonia and Respiratory Infections
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Clostridium difficile and Clostridium perfringens research
- Liver Disease Diagnosis and Treatment
- PARP inhibition in cancer therapy
- Respiratory Support and Mechanisms
- Bacterial Infections and Vaccines
- Pancreatitis Pathology and Treatment
- Intensive Care Unit Cognitive Disorders
- Clinical Nutrition and Gastroenterology
- Infective Endocarditis Diagnosis and Management
- Nutrition and Health in Aging
- Urinary Tract Infections Management
- Antibiotic Use and Resistance
- Cardiac Ischemia and Reperfusion
- Fatty Acid Research and Health
- Immune cells in cancer
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2014-2025
Universidade de São Paulo
2015-2024
Universidade Brasil
2009-2024
Hospital de Clínicas
2024
Hospital Universitário da Universidade de São Paulo
2010-2023
Hospital São Paulo
2012-2023
Universidade Cruzeiro do Sul
2021
Escuela Universitaria de Fisioterapia de la ONCE
2016
University of Almería
2016
Universidad Autónoma de Madrid
1980-2014
Patients with diabetes exhibit a high incidence of diabetic cardiomyopathy and vascular complications, which underlie the development retinopathy, nephropathy, neuropathy increase risk hypertension, stroke, myocardial infarction. There is emerging evidence that activation nuclear enzyme poly(ADP-ribose) polymerase (PARP) importantly contributes to endothelial dysfunction in streptozotocin-induced model diabetes. We investigated role PARP pathogenesis cardiac genetic (nonobese diabetic)...
Gram-negative sepsis is mediated by the actions of proinflammatory genes induced in response to microbes and their products. We report that flagellin, monomeric subunit flagella, a potent species released Salmonella. Flagellin (1 microgram/ml) induces IkappaBalpha degradation, NF-kappaB nuclear translocation, inducible NO synthase expression cultured intestinal epithelial cells (IEC). Aflagellic Salmonella mutants do not induce activation or production IEC. Antiserum flagellin blocks IEC...
Recent studies demonstrated that activation of the nuclear enzyme poly(ADP-ribose) polymerase-1 (PARP-1) by oxidant-mediated DNA damage is an important pathway tissue injury in conditions associated with oxidative stress. Using a dual approach PARP-1 suppression, genetic deletion or pharmacological inhibition phenanthridinone PARP inhibitor PJ-34, we now demonstrate essential role development pulmonary inflammation induced lipopolysaccharide (LPS). PARP-1+ / + and PARP-1− − mice received...
To describe the composition of metabolic acidosis in patients with severe sepsis and septic shock at intensive care unit admission throughout first 5 days stay.Prospective, observational study.Twelve-bed unit.Sixty either or shock.None.Data were collected until after admission. We studied contribution inorganic ion difference, lactate, albumin, phosphate, strong gap to acidosis. At admission, standard base excess was -6.69 +/- 4.19 mEq/L survivors vs. -11.63 4.87 nonsurvivors (p < .05);...
Oxygen- and nitrogen-derived free radicals oxidants play an important role in the pathogenesis of diabetic endothelial dysfunction. Recently we proposed importance oxidant-induced DNA strand breakage activation nuclear enzyme poly(ADP-ribose) polymerase (PARP) In this study, tested whether established dysfunction is reversible by PARP inhibition. The novel inhibitor PJ34 (10 mg/kg per day PO) was given at various lengths (4 weeks or 3 days) for streptozotocin-diabetic animals. addition, also...
Objective To synthesize novel inhibitors of the nuclear enzyme poly(adenosine 5′-diphosphate [ADP]-ribose) synthetase (PARS), also known as poly(ADP-ribose) polymerase (PARP), and to test them in vitro models oxidant-induced cytotoxicity endotoxin splanchnic occlusion-reperfusion-induced shock. Design Randomized, prospective laboratory study. Setting Research laboratory. Subjects Murine macrophages, thymocytes, endothelial cells; Balb/c mice Wistar rats. Interventions Macrophages cells were...
In this study, we have evaluated the impact of methodological approaches in determination biofilm formation by four clinical isolates Escherichia coli static assays.The assays were performed microtitre plates with two minimal and enriched broths, one- or two-steps protocol, using three different mathematical formulas to quantify adherent bacteria. Different patterns found depending on E. strain, culture medium reading optical density protocol. Strong moderate occurred mostly media. The used...
Sepsis is associated with increased production of superoxide and nitric oxide, consequent peroxynitrite generation. Cardiodepression induced in the heart during oxidative stress septic shock. Oxidative nitrosative can lead to activation nuclear enzyme poly(adenosine 5'-diphosphate [ADP]-ribose) polymerase (PARP), subsequent loss myocardial contractile function. The aim study was investigate whether cardiodepression found patients plasma markers necrosis PARP activation.Prospective...
Hemorrhagic shock (HS) and resuscitation leads to widespread production of oxidant species. Activation the enzyme poly(ADP-ribose) polymerase (PARP) has been shown contribute cell necrosis organ failure in various disease conditions associated with oxidative stress. We tested hypothesis whether PARP activation plays a role multiple dysfunction complicating HS murine model by using mice genetically deficient (PARP(-/-)) their wild-type littermates (PARP(+/+)). Animals were bled mean blood...
Peroxynitrite is a cytotoxic oxidant formed from nitric oxide (NO) and superoxide. Tyrosine nitration, footprint of peroxynitrite, has been demonstrated in the pancreatic islets as well cardiovascular system diabetic subjects. Delineation pathogenetic role peroxynitrite disease conditions requires use potent, vivo active decomposition catalysts. The aim current work was to produce potent catalyst test its effects rodent models diabetes complications. FP15 synthesized analyzed using standard...
The susceptibilities of 265 strains Corynebacterium species and other non-spore-forming gram-positive bacilli to 18 antimicrobial agents were tested. Most susceptible vancomycin, doxycycline, fusidic acid. jeikeium urealyticum the most resistant organisms Resistance beta-lactams, clindamycin, erythromycin, azythromycin, ciprofloxacin gentamicin was common among xerosis minutissimum. Ampicillin resistance Listeria monocytogenes more prevalent than previously reported. Optochin, fosfomycin,...
Sepsis is associated with a widespread production of proinflammatory cytokines and various oxidant species. Activation the enzyme poly(ADP-ribose) polymerase (PARP) has been shown to contribute cell necrosis organ failure in diseases inflammation reperfusion injury. The aim current study was elucidate role PARP activation multiple dysfunction complicating sepsis murine model polymicrobial induced by cecal ligation puncture (CLP). Mice genetically deficient (PARP−/−) their wild-type...
Background: The incorporation of lipid emulsions in parenteral diets is a requirement for energy and essential fatty acid supply to critically ill patients. In this study, the toxicity emulsion rich (60%) triacylglycerol ω‐6 polyunsaturated acids on leukocytes from healthy volunteers was investigated. Methods: Eleven were recruited, blood samples collected before infusion soybean oil emulsion, immediately afterwards, 18 hours later. cells studied after isolation again 24 or 48 culture....
Abstract Background Crohn's disease (CD) is a high morbidity chronic inflammatory disorder of unknown aetiology. Adherent-invasive Escherichia coli (AIEC) has been recently implicated in the origin and perpetuation CD. Because bacterial biofilms gut mucosa are suspected to play role CD biofilm formation feature certain pathogenic E. strains, we compared capacity 27 AIEC 38 non-AIEC strains isolated from intestinal mucosa. Biofilm was then contrasted with phenotype, serotype, phylotype,...
In four patients with alkaline-encrusted cystitis, Corynebacterium group D2 was isolated from consecutive urine cultures and stones. Encrusted cystitis occurred in bladders harboring inflammatory or tumorous lesions chronic recurrent urinary tract infections appearing after surgery instrumentation. The urease activity of the neutralization this enzyme by acetohydroxamic acid are shown. Clinical improvement, disappearance struvite crystals, decrease pH were obtained when these bacteria...