A5059377141- Escherichia coli research studies
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Mycobacterium research and diagnosis
- Probiotics and Fermented Foods
- Radiopharmaceutical Chemistry and Applications
- Colorectal Cancer Screening and Detection
- Clostridium difficile and Clostridium perfringens research
- Global Cancer Incidence and Screening
- Radioactive element chemistry and processing
- Boron Compounds in Chemistry
- Evolution and Genetic Dynamics
- Bacterial Genetics and Biotechnology
- Cancer Risks and Factors
Universitat de Girona
2018-2023
Institut d'Investigació Biomèdica de Girona
2014
Background & Aims Adherent-invasive E. coli (AIEC) has largely been implicated in the pathogenesis of Crohn’s disease (CD). strains with similar genetic backgrounds and virulence genes profiles have associated other intestinal disorders, such as ulcerative colitis (UC), colorectal cancer (CRC), coeliac (CeD), but role AIEC these diseases remains unexplored. We aimed to assess distribution, abundance, pathogenic features UC, CRC, CeD. Methods The phenotype was investigated 4,233...
Adherent-invasive Escherichia coli (AIEC) have been involved in Crohn's disease (CD). Currently, AIEC are identified by time-consuming techniques based on vitro infection of cell lines to determine their ability adhere and invade intestinal epithelial cells as well survive replicate within macrophages. Our aim was find signature sequences that can be used identify the pathotype. Comparative genomics performed between three E. strain pairs, each pair comprised one non-AIEC with identical...
To date no molecular tools are available to identify the adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease and colonizes intestine of different hosts. Current techniques based on phenotypic screening isolates extremely time-consuming. The aim this work was search for signature traits assist in rapid AIEC identification. occurrence at least 54 virulence genes (VGs), resistance 30 antibiotics distribution FimH ChiA amino acid substitutions...
Increasingly, tcdA/tcdB qPCR is being used to diagnose Clostridioides difficile infections (CDI). Under the hypothesis that toxin genes' quantification through Ct values could potentially improve clinical accuracy, this study aimed assess linearity of C. assay on QIAstat-Dx® Gastrointestinal Panel 2 (GI2 Panel) and correlate bacterial toxins load. Four analytical thirty-five toxigenic isolates were quantified using three validated standard curve assays targeting adK, tcdA tcdB genes. Of...
The salts Na[ROC(O)Ph], Na[1-ROC(O)-1,12-C2B10H11], K2[1,4-(ROC(O))2-C6H4], and K2[1,12-(ROC(O))2-1,12-C2B10H10], where R is the cobaltabis(dicarbollide)-diethylene glycol group [3,3′-Co(8-(OCH2CH2OCH2CH2)-1,2-C2B9H10)(1′,2′-C2B9H11)]−, were synthesized from corresponding carboxylate salt zwitterion [3,3′-Co(8-(CH2CH2O)2-1,2-C2B9H10)(1′,2′-C2B9H11)]. dianion in K2[1,12-(ROC(O))2-1,12-C2B10H10] showed at least one K+ cation to be tightly bound via K···O K···H–B interactions with both...
Adherent-invasive Escherichia coli (AIEC) is strongly associated with the pathogenesis of Crohn's disease (CD). However, no molecular markers currently exist for AIEC identification. This study aimed to identify differentially expressed genes (DEGs) between and non-AIEC strains that may contribute pathogenicity evaluate their utility as markers.Comparative transcriptomics was performed on two closely related AIEC/non-AIEC strain pairs during Intestine-407 cell infection. DEGs were quantified...
Variations in the sequence and/or expression of outer membrane proteins (OMPs) may modulate bacterial virulence. OmpA and OmpC have been involved interaction adherent-invasive Escherichia coli (AIEC) strain LF82 with intestinal epithelial cells (IECs). Scarce data exist about OMPs variants a collection AIEC strains, no study during infection exists. We aimed to determine whether particular mutations or differential are associated The ompA, ompC, ompF genes 14 30 non-AIEC strains were...
Abstract Adherent-invasive Escherichia coli (AIEC) have been extensively implicated in Crohn’s disease pathogenesis. Currently, AIEC is identified phenotypically, since no molecular marker specific for exists. An algorithm based on single nucleotide polymorphisms was previously presented as a potential tool to classify AIEC/non-AIEC, with 84% accuracy collection of 50 strains isolated Girona (Spain). Herein, our aim determine the using AIEC/non-AIEC isolates from different geographical...
Abstract Background Adherent invasive Escherichia coli (AIEC) have been related to Crohn’s disease. Phenotypic assays of adhesion and invasion intestinal epithelial cells intra-macrophage replication are used identify AIEC. No molecular tools still available for AIEC identification. We hypothesise that differential gene expression may drive the phenotype. To test this hypothesis we sequenced genes expressed by non-AIEC strains during infection, investigated (DEGs) suitability as markers....