A5101703286- Effects and risks of endocrine disrupting chemicals
- Pharmacogenetics and Drug Metabolism
- Toxic Organic Pollutants Impact
- Eicosanoids and Hypertension Pharmacology
- Alcohol Consumption and Health Effects
- Estrogen and related hormone effects
- Drug Transport and Resistance Mechanisms
- Environmental Toxicology and Ecotoxicology
- Metabolism and Genetic Disorders
- Carcinogens and Genotoxicity Assessment
- Pesticide Exposure and Toxicity
- Drug-Induced Hepatotoxicity and Protection
- Nitric Oxide and Endothelin Effects
- Analytical Chemistry and Chromatography
- Sulfur Compounds in Biology
- Inflammatory mediators and NSAID effects
- Metabolomics and Mass Spectrometry Studies
- Amino Acid Enzymes and Metabolism
- Bone Tissue Engineering Materials
- Chemical Reactions and Isotopes
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- Aldose Reductase and Taurine
- Free Radicals and Antioxidants
- Olfactory and Sensory Function Studies
Nihon Pharmaceutical University
2014-2025
Hiroshima University
2002-2012
Hiroshima International University
2012
PhoenixBio (Japan)
2012
Nagoya University
2007-2012
Xenobe Research Institute
2006
National Institute of Advanced Industrial Science and Technology
2003-2004
Nippon Medical School
2000-2004
Maruho (Japan)
1999
Kyoto Research Park
1999
The endocrine-disrupting activities of bisphenol A (BPA) and 19 related compounds were comparatively examined by means different in vitro vivo reporter assays. BPA some exhibited estrogenic activity human breast cancer cell line MCF-7, but there remarkable differences activity. Tetrachlorobisphenol (TCBPA) showed the highest activity, followed B, BPA, tetramethylbisphenol (TMBPA); 2,2-bis(4-hydroxyphenyl)-1-propanol, 1,1-bis(4-hydroxyphenyl)propionic acid 2,2-diphenylpropane little or no...
An increasing number of studies are reporting the existence polybrominated diphenyl ethers (PBDEs) and their hydroxylated (HO) methoxylated (MeO) metabolites in environment tissues from wildlife humans.Our aim was to characterize compare agonistic antagonistic activities principle PBDE congeners HO MeO against human nuclear hormone receptors.We tested receptor estrogen alpha (ERalpha), ERbeta, androgen (AR), glucocorticoid (GR), thyroid alpha(1) (TRalpha(1)), TRbeta(1) BDEs 15, 28, 47, 85,...
To develop novel nonallergenic pyrazolone analgesics, we synthesized a series of compounds in which position 1 the ring was substituted place original methyl group order to block formation allergenic metabolites via N-dealkylation. These analogues were found show as potent an antipyretic and analgesic effect antipyrine (AT). In examination allergenicity, AT induced typical skin reaction guinea pigs, whereas inactive. When administered (po) rats, norantipyrine (NORA) active metabolite...
Indoor dust is a sink for many kinds of pollutants, including flame retardants (FRs), plasticizers, and their contaminants degradation products. These pollutants can be migrated to indoor from household items such as televisions computers. To reveal high-priority end points contaminant candidates in dust, using CALUX reporter gene assays based on human osteosarcoma (U2OS) cell lines, we evaluated characterized the endocrine-disrupting potencies crude extracts collected Japan (n = 8), United...
In order to investigate the effect of sunlight on persistence and ecotoxicity pharmaceuticals contaminating aquatic environment, we exposed nine (acetaminophen (AA), amiodarone (AM), dapsone (DP), dexamethasone (DX), indomethacin (IM), naproxen (NP), phenytoin (PH), raloxifene (RL), sulindac (SL)) in aqueous media ultraviolet (UV) irradiation at 254, 302 or 365 nm (UV-C, UV-B UV-A, respectively). Degradation was monitored by means high-performance liquid chromatography (HPLC). Sunlight...
The non-structural X protein, HBx, of hepatitis B virus (HBV) is assumed to play an important role in HBV replication. Woodchuck protein indispensable for replication, but the duck not. In this study, we investigated whether HBx replication vivo using human hepatocyte chimeric mice. HBx-deficient (HBx-def) was generated HepG2 cells by transfection with overlength genome. Human mice were infected HBx-def or without hepatic expression hydrodynamic injection plasmids. Serum levels and sequences...
The affinity for thyroid hormone receptor (TR) of polybromodiphenyl ethers (PBDEs) and hydroxylated PBDEs was examined. 4-Hydroxy-2,2',3,4',5-pentabromodiphenyl ether (4-OH-BDE-90) 3-hydroxy-2,2',4,4'-tetrabromodiphenyl (3-OH-BDE-47) markedly inhibited the binding triiodothyronine (1×10-10 M) to TR in concentration range 1×10-6-1×10-4 M. 2,3,4,5,6-Pentabromophenol (PBP) also showed an inhibitory effect at 1×10-5-1×10-4 However, 2,2',3,4,4',5'-hexabromodiphenyl (BDE-138), decabromodiphenyl...
Accurate prediction of pharmacokinetics (PK) parameters in humans from animal data is difficult for various reasons, including species differences. However, chimeric mice with humanized liver (PXB mice; urokinase-type plasminogen activator/severe combined immunodeficiency repopulated approximately 80% human hepatocytes) have been developed. The expression levels and metabolic activities cytochrome P450 (P450) non-P450 enzymes the livers PXB are similar to those humans. In this study, we...
The in vitro metabolism of <i>p</i>,<i>p</i>′-DDT [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane], an important environmental pollutant, was examined rat liver, focusing on reductive dechlorination. When incubated with liver microsomes rats the presence NADPH or NADH, a dechlorinated metabolite,<i>p</i>,<i>p</i>′-DDD [1,1-dichloro-2,2-bis(4-chlorophenyl)ethane], formed under anaerobic conditions together dehydrochlorinated metabolite,<i>p</i>,<i>p</i>′-DDE...
Abstract This study deals with individual and species variations in the converting activity of methotrexate (MTX) to 7‐hydroxymethotrexate animals humans. When MTX 7‐hydroxylase was assayed six human liver cytosols, a 48‐fold range intersubject variation observed. The were correlated concentrations aldehyde oxidase subjects benzaldehyde as substrate. Species differences also highest rabbits, followed by rats, hamsters, monkeys but undetectable dogs. Strain based on observed rats mice....
Zonisamide (1,2-benzisoxazole-3-methanesulphonamide), a new anticonvulsant, is mainly metabolized to 2-sulphamoylacetylphenol by reduction of the benzisoxazole ring. Recent studies have shown that mammalian liver enzymes are responsible for zonisamide. Because intestinal bacteria can also mediate xenobiotics, this study was designed evaluate role in in-vivo reductive metabolism Treatment rats with antibiotics significantly reduced urinary and faecal excretion after oral administration...