A5006798714- Cannabis and Cannabinoid Research
- Prenatal Substance Exposure Effects
- Ectopic Pregnancy Diagnosis and Management
- Molecular Biology Techniques and Applications
- Ovarian function and disorders
- Gynecological conditions and treatments
- Animal Genetics and Reproduction
- Homelessness and Social Issues
- Sexual Differentiation and Disorders
- Uterine Myomas and Treatments
- Hormonal and reproductive studies
- Reproductive System and Pregnancy
- Gestational Trophoblastic Disease Studies
- Reproductive Health and Technologies
- Religion, Spirituality, and Psychology
- Assisted Reproductive Technology and Twin Pregnancy
- Pregnancy-related medical research
- Alcoholism and Thiamine Deficiency
- Pain Management and Opioid Use
- Neonatal and fetal brain pathology
- Ovarian cancer diagnosis and treatment
- Bone fractures and treatments
- Minimally Invasive Surgical Techniques
- Growth Hormone and Insulin-like Growth Factors
- Emergency and Acute Care Studies
John Hunter Hospital
2023
University of Newcastle Australia
2023
Sheffield Teaching Hospitals NHS Foundation Trust
2017
Royal Hallamshire Hospital
2016
University of Leicester
2010-2013
Leicester Royal Infirmary
2010-2013
National Health Service
2012-2013
University Hospitals of Leicester NHS Trust
2012-2013
Royal Victoria Infirmary
2010
Ectopic pregnancy is associated with significant morbidity and mortality, but the molecular mechanisms underlying this condition remain unclear. Although endocannabinoids, N-arachidonoylethanolamine (anandamide), N-oleoylethanolamine, N-palmitoylethanolamine, are thought to play a negative role in ectopic pregnancy, their precise role(s) within fallopian tube remains Anandamide activates cannabinoid receptors (CB1 CB2) and, together its degrading [e.g. fatty acid amide hydrolase (FAAH)]...
Studies from knockout mice suggest that perturbations in oviductal endocannabinoid levels, receptors, or degrading enzyme [fatty acid amide hydrolase (FAAH)] expression result infertility secondary to physical trapping of embryos. Similar observations have been made ectopic pregnant women together with a suggestion the receptor gene polymorphism 1359G/A (rs1049353) is associated pregnancy. These led hypothesis pregnancy perturbation levels endocannabinoids and FAAH activity such changes are...
Ultrasonography is a core skill required by all obstetrics and gynecology trainees; however, training opportunities in clinical ultrasound are declining. Simulation has been proposed as strategy to overcome this.The study aims were determine the current availability of simulation United Kingdom explore trainees' perspective on role simulation.All trainees within East Midlands Local Education Training Board asked complete an anonymous web-based survey July 2014.Of 140 trainees, 70 (50%)...
RT-qPCR is commonly employed in gene expression studies ectopic pregnancy. Most use RN18S1, β-actin or GAPDH as internal controls without validation of their suitability reference genes. A systematic study the endogenous genes for pregnancy lacking. The aims this were therefore to evaluate stability 12 and suggest those that are stable control fallopian tubes endometrium from healthy non-pregnant controls. Analysis results showed consistently ranked top six by geNorm NormFinder algorithms,...
Quantitative real-time polymerase chain reaction (qRT-PCR) has been employed to study the gene expression profiles in human spermatozoa, but accurate analysis is dependent upon normalisation of data against an endogenous control. β-Actin (ACTB) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are most commonly used reference genes for these many tissues considerable variation under different physiological, pathological experimental conditions which limits their effectiveness...
Objectives: Cannabinoids are effective antiemetics and the "endogenous cannabinoids" (endocannabinoids) thought to modulate emesis in both humans animal models. Endocannabinoids, their receptors metabolising enzymes present peripheral blood a reduction endocannabinoid concentration has been observed individuals with excessive nausea vomiting following parabolic flight manoeuvres. We tested hypothesis that plasma levels similarly perturbed women hyperemesis gravidarum (HG), condition where...
A 31-year-old woman presented with a 7-week history of irregular vaginal bleeding without abdominal pain. She had been using the intrauterine contraceptive device (IUD) for last 3 years. pregnancy test was positive and subsequent serum beta human chorionic gonadotropin (β-HCG) 4992 mIU/mL. transvaginal ultrasound scan demonstrated an empty uterus associated adnexal mass but no free fluid. right primary ovarian ectopic diagnosed laparoscopy. This managed laparoscopically monopolar diathermy...
The aim of this study was to assess cardiotocograph (CTG) interpretation by midwives and trainee obstetricians using the standard a modified definition fetal heart rate deceleration compared with consultant as Gold Standard. A randomised survey online tool at: www.surveymonkey.com between 4 January 24 April 2009, conducted at tertiary obstetric unit, UK. total 104 (∼54%) health professionals responded, providing 1,118 responses respect presence decelerations on 13 anonymised CTGs. Five...
Ectopic pregnancy is common and still associated with significant morbidity mortality, despite advances in diagnosis treatment. Most of the consequences are related to either late or missed diagnosis. Despite these consequences, a reliable biomarker yet be developed. Although classical risk factors include pelvic inflammatory disease, smoking, tubal surgery, previous ectopic number pregnancies occur women without factors. This article aims elaborate on understanding molecular biology by...