A5080554132- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Fermentation and Sensory Analysis
- Lipid Membrane Structure and Behavior
- Advanced Proteomics Techniques and Applications
- Protein Degradation and Inhibitors
- Cellular transport and secretion
- Calcium signaling and nucleotide metabolism
- Lipid metabolism and biosynthesis
- Cancer-related gene regulation
- Microbial Metabolic Engineering and Bioproduction
- Nutrition and Health in Aging
- Frailty in Older Adults
- Colorectal Cancer Surgical Treatments
- Peroxisome Proliferator-Activated Receptors
Weizmann Institute of Science
2021-2024
Shaare Zedek Medical Center
2019
Actively maintained close appositions between organelle membranes, also known as contact sites, enable the efficient transfer of biomolecules cellular compartments. Several such sites have been described well their tethering machineries. Despite these advances we are still far from a comprehensive understanding function and regulation most sites. To systematically characterize site proteomes, established high-throughput screening approach in Saccharomyces cerevisiae based on co-localization...
Contact sites are areas of close apposition between two membranes that coordinate nonvesicular communication organelles. Such interactions serve a wide range cellular functions from regulating metabolic pathways to executing stress responses and coordinating organelle inheritance. The past decade has seen dramatic increase in information on certain contact sites, mostly those involving the endoplasmic reticulum. However, despite its central role secretory pathway, Golgi apparatus remain...
Abstract Genome-wide collections of yeast strains, known as libraries, revolutionized the way systematic studies are carried out. Specifically, libraries that involve a cellular perturbation, such deletion collection, have facilitated key biological discoveries. However, short-term rewiring and long-term accumulation suppressor mutations often obscure functional consequences perturbations. We present AID library which supplies “on demand” protein depletion to overcome these limitations....
Genome-wide collections of yeast strains, known as libraries, revolutionized the way systematic studies are carried out. Specifically, libraries that involve a cellular perturbation, such deletion collection, have facilitated key biological discoveries. However, short-term rewiring and long-term accumulation suppressor mutations often obscure functional consequences perturbations. We present AID library which supplies “on demand” protein depletion to overcome these limitations. Here, each is...
Peroxisomes host essential metabolic enzymes and are crucial for human health survival. Although peroxisomes were first described over 60 years ago, their entire proteome has not yet been identified. As a basis understanding the variety of peroxisomal functions, we used high-throughput screen to discover proteins in yeast. To visualize low abundance proteins, utilized collection strains containing marker which each protein is expressed from constitutive strong TEF2 promoter. Using this...
Abstract Actively maintained close appositions, or contact sites, between organelle membranes, enable the efficient transfer of biomolecules various cellular compartments. Several such sites have been described together with their tethering machinery. Despite these advances we are still far from a comprehensive understanding function and regulation most sites. To systematically characterize proteome support discovery new tethers functional molecules, established high throughput screening...