A5075544469- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Atherosclerosis and Cardiovascular Diseases
- Atrial Fibrillation Management and Outcomes
- Immune Response and Inflammation
- Protease and Inhibitor Mechanisms
- Chemokine receptors and signaling
- Cardiac Arrhythmias and Treatments
- Hormonal Regulation and Hypertension
- Immunotherapy and Immune Responses
- Transplantation: Methods and Outcomes
- Antioxidant Activity and Oxidative Stress
- Health Systems, Economic Evaluations, Quality of Life
- Cardiac electrophysiology and arrhythmias
- Apelin-related biomedical research
- Cardiac and Coronary Surgery Techniques
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immune cells in cancer
- Acute Myocardial Infarction Research
- Electrospun Nanofibers in Biomedical Applications
- Heart Failure Treatment and Management
- Vitamin C and Antioxidants Research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Lipoproteins and Cardiovascular Health
Liverpool Heart and Chest Hospital
2022
Aalborg University
2022
University of Liverpool
2022
Laboratoire Informatique d'Avignon
2022
Centre Hospitalier Universitaire de Tours
2021
Thion Medical (France)
2021
Carol Davila University of Medicine and Pharmacy
2021
Ludwig-Maximilians-Universität München
2005-2018
Massachusetts Institute of Technology
2005-2018
Harvard–MIT Division of Health Sciences and Technology
2004-2012
Atherosclerosis is an inflammatory disease in which monocytes and macrophages have been suggested to play essential role. The underlying signaling mechanisms are unknown thus far. We hypothesized that the human isoform of Toll-like receptor (hTLR)-4 involved monocyte activation patients with accelerated forms atherosclerosis.Expression hTLR4 on circulating from 30 controls, 20 stable angina (SA), 40 unstable (UA), 28 acute myocardial infarction (AMI) was compared use flow-cytometry reverse...
Anti-inflammatory effects of statins contribute to their clinical benefit. Molecular mechanisms underlying these have not been well explored. Because attenuate lipopolysaccharide (LPS) responsiveness, we hypothesized that part the pleiotropic are mediated through innate immunity.Toll-like receptor (TLR) 4 expression and downstream signaling in CD14+ monocytes after incubation with simvastatin atorvastatin were quantified via flow-cytometry, quantitative RT-PCR, kinase assay, enzyme-linked...
Background. Long-term success of cardiac transplantation is limited by various forms graft rejection. The specific mechanisms initiating and controlling these highly specialized immune processes remain unclear so far. Methods. To investigate the role innate immunity in development allograft rejection, we assessed toll-like receptor (TLR)4 expression typical downstream effects TLR signaling (B7–1, interleukin [IL]-12, tumor necrosis factor [TNF]-α) circulating CD14+ monocytes 38 transplant...
Endothelial cell phenotypes markedly differ, depending upon function and vascular bed of origin. Differences might account for specific susceptibility to pathological conditions. As leukocyte adhesion activated endothelium is the initiating event in a range diseases, we compared influence bed-specific flow patterns on molecule expression human saphenous vein (HSVEC) coronary artery endothelial cells (HCAEC). In vitro, immune attachment was increased 1.6-fold when tumor necrosis factor...
Background— Endothelial cell (EC) dysfunction represents the first manifestation of atherosclerotic disease. Restoration endothelium via seeding or transfection is hampered by local alterations in flow, inflammation, and metabolic activation. Perivascular EC matrix implants are shielded from these forces still control vascular repair. The host immune response to such implants, however, remains largely unknown. We investigated effect embedding ECs within 3-dimensional matrices on responses...
The endothelium is a highly specialized active interface between blood and the underlying tissues, maintaining vascular tone, thrombo-resistance selective permeability to cells proteins. It also an important regulator of inflammatory diseases, endothelial-leukocyte interactions often herald complex diseases with component. Yet, exact mechanisms promoting immune activation endothelial (EC) are incompletely understood. Knowledge accumulating that immediate environment defines cellular...
The fate of allo- and xenogeneic endothelial cell (EC) implants is regulated by EC-matrix interactions. While free EC are destroyed a vigorous immune reaction, embedded within 3D collagen cells well tolerated. Given the critical role DC serve in reactivity, we hypothesized that EC-driven maturation depends on contact. In marked contrast to co-cultured with cytokine cocktail or grown confluence 2D tissue culture plates, exposed matrix-embedded failed mature, retaining their endocytic activity...
ABSTRACT Endothelial integrity regulates vascular tone, luminal patency, and the immune reactivity to tissue grafts. dysfunction is first marker site of disease initiation severity. It has long been known that endothelial biochemical function density dependent, we have recently shown immunobiology anchorage dependent. Matrix‐embedded cells (EC) establish a controlled state are not only protected but also induce system protective state. We now define this aspect biology in detail. The vitro...
Platelets are playing a crucial role in acute cardiovascular events. We investigated if physical stress activates platelets and whether this activation can be inhibited by polyphenol-enriched diet.
Autoimmunity may exacerbate vascular disease, particularly in the form of anti-endothelial cell (EC) antibodies. The increased morbidity cardiovascular diseases concert with diabetes mellitus, hypertension, and other systemic illnesses reflect increase presence potency these Matrix-embedded ECs act as powerful regulators repair accompanied by significant reduction expected local inflammation. We compared immune response against free matrix-embedded naïve mice heightened EC reactivity.Mice...
Because changes in subendothelial matrix composition are associated with alterations of the endothelial immune phenotype, we sought to understand if cytokine-induced NF-κB activity and downstream effects depend on substrate adherence cells (EC). We compared upstream phosphorylation cascade, activation NF-κB, expression/secretion EC grown tissue culture polystyrene plates (TCPS) embedded within collagen-based matrices (MEEC). Adhesion natural killer (NK) was quantified vitro vivo. subunit p65...
Stress is associated with increased susceptibility to infection. We investigated if the mechanism involves immunomodulation of dendritic cells and whether this can be inhibited by a polyphenol-rich diet. Blood samples were taken from total 100 male endurance athletes at 5 time points around marathon run: 4 weeks before; 1 week immediately, 24 h, 72 h after. Participants randomized into 2 double-blinded groups. One group received beverage during 3-week training phase before while other...
The programmed form of cell death (apoptosis) is essential for normal development multicellular organisms. Dysregulation apoptosis has been linked with embryonal and involved in the pathophysiology various diseases. Specifically, endothelial plays pivotal roles atherosclerosis whereas prevention a prerequisite neovascularization tumors metastasis. Endothelial biology intertwined composition subendothelial basement membrane proteins. Apoptosis was induced by addition tumor necrosis factor-α...