A5047549979- Laser Applications in Dentistry and Medicine
- Pain Management and Treatment
- Laser Material Processing Techniques
- Indoor Air Quality and Microbial Exposure
- Infection Control and Ventilation
- Ultrasound and Cavitation Phenomena
- Air Quality and Health Impacts
- Prenatal Screening and Diagnostics
- Ocular and Laser Science Research
- Biosensors and Analytical Detection
- Laser Design and Applications
- Laser-induced spectroscopy and plasma
- Chromosomal and Genetic Variations
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- HER2/EGFR in Cancer Research
- Genomics and Phylogenetic Studies
- Photoreceptor and optogenetics research
- Ergonomics and Musculoskeletal Disorders
- Infection Control in Healthcare
- Laser-Ablation Synthesis of Nanoparticles
- CRISPR and Genetic Engineering
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
Bio-Rad (United States)
2011-2018
Lawrence Livermore National Laboratory
1996-2009
Digital PCR enables the absolute quantitation of nucleic acids in a sample. The lack scalable and practical technologies for digital implementation has hampered widespread adoption this inherently powerful technique. Here we describe high-throughput droplet (ddPCR) system that processing ∼2 million reactions using conventional TaqMan assays with 96-well plate workflow. Three applications demonstrate massive partitioning afforded by our ddPCR provides orders magnitude more precision...
Using aerosol-based tracers to estimate risk of infectious aerosol transmission aids in the design buildings with adequate protection against transmissible pathogens, such as SARS-CoV-2 and influenza. We propose a method for scaling bulk quantitative microbial assessment (QMRA) model impulse emissions, coughing or sneezing, aerosolized synthetic DNA tracer concentration measurements. With point-of-emission ratios describing relationships between respiratory emission characteristics (i.e.,...
Tissue ablation with ultrashort laser pulses offers several unique advantages. The nonlinear energy deposition is insensitive to tissue type allowing this tool be used for soft and hard ablation. localized leads precise depth minimal collateral damage. In paper we will report on our efforts study demonstrate using an pulse laser. efficiency, extent of damage 0.3 ps 1000 duration compared. Temperature measurements the rear surface a tooth section also presented.
An in vitro study of laser tissue welding mediated with a dye-enhanced protein solder was performed. Freshly harvested sections porcine aorta were used for the experiments. Arteriotomies approximately 4 mm length treated using an 805 nm continuous-wave diode coupled to 1-mm diameter fiber. Temperature histories surface weld site obtained fiberoptic-based infrared thermometer. The experimental effort complemented by LATIS (LAser-TISsue) computer code, which numerically simulates exposure...
Objective: This study examines the use of dye-enhanced protein bonding agents in 805 nm diode laser-assisted tissue welding. A comparison an albumin liquid solder and collagen solid-matrix patches used to repair arteriotomies vitro porcine model is presented. Summary background data: Extrinsic media form solders have been enhance practice laser Preferential absorption wavelength has achieved by incorporation chromophores. Methods: Both patch included indocyanine green dye (ICG) absorb...
A study of laser tissue welding mediated with an indocyanine green dye-enhanced protein solder was performed. Freshly obtained sections porcine artery were used for the experiments. Sample arterial wall thickness ranged from two to three millimeters. Incisions approximately four millimeters in length treated using 805 nanometer continuous- wave diode coupled a one millimeter diameter fiber. Controlled parameters included power delivered by laser, duration process, and concentration dye...
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An in vivo study of vascular welding with a fiber-delivered argon laser was conducted using canine model. Longitudinal arteriotomies and venotomies were treated on femoral vein artery. Laser energy delivered to the vessel wall via 400 micrometer optical fiber. The surface temperature at center spot monitored real time hollow glass fiber-based two-color infrared thermometer. limited by either room-temperature saline drip or direct feedback control mechanical shutter alternately pass block...
We have implemented an interferometric method to probe the dynamics of transients induced in absorbing water solutions with nanosecond laser pulses delivered through optical fiber. examined two cases corresponding strong absorption (deposition within 30 micrometers fiber tip) and moderate 500 tip). With deposited energy leads a stress transient followed by development thermally generated vapor bubble. Moderate formation cavitation bubble cloud arising from tensile stresses that are produced fluid.
Optically generated acoustic waves have been used to temporarily permeate biological cells. This technique may be useful for enhancing transfection of DNA into cells or the absorption locally delivered drugs. A diode- pumped frequency-doubled Nd:YAG laser operating a kHz repetition rates was produce series pulses. An wave formed via thermoelastic expansion by depositing radiation an absorbing dye. Generated pressures were measured with PVDF hydrophone. The transmitted culture and plated cell...
The strength and stability of laser-welded tissue may be influenced, in part, by the effects laser exposure on collagen crosslinking. We therefore studied diode (805 nm, 1 - 8 watts, 30 seconds) plus indocyanine green dye (ICG) calf tail tendon crosslinks. effect ICG alone crosslink content prior to was investigated; unexpectedly, we found that ICG-treated had significantly increased DHLNL OHP, but not HLNL. Laser after application reduced elevated OHP down their native levels....