A5026603719- Plant Molecular Biology Research
- Advances in Cucurbitaceae Research
- Plant Reproductive Biology
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Cocoa and Sweet Potato Agronomy
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Plant Physiology and Cultivation Studies
- Plant nutrient uptake and metabolism
- Water Quality and Pollution Assessment
- Marine and coastal ecosystems
- Food composition and properties
- Cellular transport and secretion
- Light effects on plants
- Plant Disease Resistance and Genetics
- Fish Ecology and Management Studies
- Plant Disease Management Techniques
- Signaling Pathways in Disease
- biodegradable polymer synthesis and properties
- Plant Diversity and Evolution
- Microbial Community Ecology and Physiology
Qingdao Agricultural University
2020-2025
Inner Mongolia University
2024
Ministry of Agriculture and Rural Affairs
2024
Shandong Agricultural University
2013-2020
Abstract Structural variants (SVs) represent a major source of genetic diversity and are related to numerous agronomic traits evolutionary events; however, their comprehensive identification characterization in cucumber ( Cucumis sativus L.) have been hindered by the lack high-quality pan-genome. Here, we report graph-based pan-genome analyzing twelve chromosome-scale genome assemblies. Genotyping seven large chromosomal rearrangements based on provides useful information for use wild...
ABSTRACT Transgenic and gene‐editing technologies are essential for gene functional analysis crop improvement. However, the pleiotropic effects unknown mechanisms of morphogenic genes have hindered their broader application. In this study, we employed one‐step de novo shoot organogenesis (DNSO) method, demonstrated that overexpression Arabidopsis thanalia GROWTH‐REGULATING FACTOR 5 ( AtGRF5 ) significantly enhanced genetic transformation efficiency in cucurbit crops by promoting callus...
Abstract The basic helix–loop–helix (bHLH) family of transcription factors (TFs) participate in a variety biological regulatory processes plants, and have undergone significant expansion during land plant evolution by gene duplications. In cucurbit crops, several bHLH genes been found to be responsible for agronomic traits such as bitterness. However, the characterization across genomes species has not reported, how they evolved diverged remains largely unanswered. Here we identified 1,160...
Abstract The use of heterografts is widely applied for the production several important commercial crops, but molecular mechanism graft union formation remains poorly understood. Here, cucumber grafted onto pumpkin was used to study development, and genome-wide tempo-spatial gene expression at interface comprehensively investigated. Histological analysis suggested that resumption rootstock growth occurred after both phloem xylem reconnection, scion showed evident callus compared with 3 days...
Spraying N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU), an exogenous cytokinin (CK) growth regulator, is the conventional method for inducing fruit set during melon (Cucumis melo L.) production; however, mechanism by which CPPU induces unclear. Through histological and morphological observations, size was comparable between CPPU-induced fruits normal pollinated because had higher cell density but smaller compared with fruits. promotes accumulation of gibberellin (GA) auxin decreases level...
Summary Polar growth of root hairs is critical for plant survival and requires fine‐tuned Rho plants ( ROP ) signaling. Multiple regulators participate in hair growth. However, protein S ‐acyl transferases PAT s), mediating the ‐acylation membrane partitioning s, are yet to be found. Using a reverse genetic approach, combining fluorescence probes, pharmacological drugs, site‐directed mutagenesis analysis with related root‐hair mutants, we have identified characterized an Arabidopsis , which...
A number of cis-regulatory elements (CREs) conserved during evolution have been found to be responsible for phenotypic novelty and variation. Cucurbit crops such as cucumber (Cucumis sativus), watermelon (Citrullus lanatus), melon melo), squash (Cucurbita maxima) develop fruits from an inferior ovary share some similar biological processes fruit development. Whether regulatory sequences play critical roles in development cucurbit remains explored. In six well-studied species, we identified...
Trans-species polymorphisms (TSPs) are fundamental to preserving ancient genetic diversity, yet the evolutionary forces driving their long-term maintenance remain largely unexplored. Here, we investigate genome-wide TSPs in two Cucumis species, cucumber and melon, using whole-genome sequencing data from over 1200 accessions. A total of 5149 were identified, which predominantly located genic promoter regions. Coalescent analysis indicated that both gene flow balancing selection have...
Cucurbits (Cucurbitaceae) include major horticultural crops with high nutritional and economic value that also serve as model plants for studying plant development crop improvement. Conventional breeding methods have made important contributions to the production of cucurbit but led a bottleneck because narrow genetic bases low variation rates these crops. With molecular techniques, innovations in germplasm through transgenesis gene editing breakthroughs breeding. Although transformation...
Prenylation, the post-translational attachment of prenyl groups to substrate proteins, can affect their distribution and interactomes. Arabidopsis PLURIPETALA (PLP) encodes shared α subunit two heterodimeric protein isoprenyltransferases, whose functional loss provides a unique opportunity study developmental cellular processes mediated by its prenylated substrates, such as ROP GTPases. As molecular switches, activation ROPs are various factors, including guanine nucleotide exchange GTPase...
Crop yield is sensitive to salt stresses, for which Calcineurin B-like proteins (CBLs) are major response factors. This study shows that Arabidopsis CBL10, through protein S-acylation by S-acyl transferase10, targets the vacuolar membrane confer tolerance. Protein a reversible posttranslational modification involves addition of 16-carbon saturated lipid group, usually palmitate cysteine residues substrate (Hemsley and Grierson 2008; Hemsley et al. 2013; Running 2014). plays important roles...
Asymmetric root growth (ARG) on tilted plates, or coiling horizontally placed is proposed to be a combination of gravitropism, mechanical sensing, and "circumnutation," word designated by Charles Darwin describe the helical movement that all plant organs make around direction. ARG developmentally controlled in which microtubule-regulating proteins phytohormone auxin participates. Nutrient deficiency influences ARG. However, it unclear nutrients play key roles regulating ARG, what endogenous...
Many biotic or abiotic factors such as CPPU (N-(2-chloro-pyridin-4-yl)-N'-phenylurea), a growth regulator of numerous crops, can induce bitterness in cucurbits. In melon, cucurbitacin B is the major compound leading to bitterness. However, molecular mechanism underlying CuB biosynthesis response different conditions remains unclear. Here, we identified set genes involved CPPU-induced melon fruit and proposed CmBr gene regulator. Using CRISPR/Cas9 editing, confirmed CmBr's role regulating...
High-throughput genotyping tools can effectively promote molecular breeding in crops. In this study, by target sequencing (GBTS) system was utilized to develop a genome-wide liquid SNP chip for facilitating genetics and melon (Cucumis melo L.), globally cultivated economically important horticultural crop. Based on over eight million SNPs derived from 823 representative accessions, 16K, 8K, 4K, 2K, 1K, 500, 250 125 informative were screened evaluated their polymorphisms, conservation of...
Sex determination in plants gives rise to unisexual flowers. A better understanding of the regulatory mechanism underlying production flowers will illuminate sex and allow researchers farmers harness heterosis. Androecious cucumber (Cucumis sativus L.) can be used as male parent when planted alongside a gynoecious line produce heterozygous seeds, thus decreasing cost seed production. The isolation characterization additional androecious genotypes varied backgrounds increase pool available...
Abstract Most eukaryotic cells are polarized. Common toolbox regulating cell polarization includes Rho guanosine triphosphatases (GTPases), in which spatiotemporal activation is regulated by a plethora of regulators. plants (ROPs) the only GTPases plants. Although vesicular trafficking was hinted regulation ROPs, it unclear where vesicle‐carried ROP starts, whether dynamically regulated, and components participate vesicle‐mediated targeting. In addition, although vesicle guanine nucleotide...
Ovules are female reproductive organs of angiosperms, containing sporophytic integuments and gametophytic embryo sacs. After fertilization, sacs develop into embryos endosperm whereas seed coat. Ovule development is regulated by transcription factors (TF) whose expression often controlled microRNAs. Mutations Arabidopsis DICER-LIKE 1 (DCL1), a microRNA processing protein, caused defective ovule reduced fertility. However, it was not clear whether other proteins participate in this process...
Abstract Background Chloroplasts are essential organelles of plant cells for not only being the energy factory but also making adaptable to different environmental stimuli. The nuclear genome encodes most chloroplast proteins, among which a large percentage membrane proteins have yet be functionally characterized. Results We report here functional characterization two nuclear-encoded Chloroplast protein Growth and Fertility (CGF1) CGF2. CGF1 CGF2 expressed in diverse tissues developmental...