A5082371277- Metabolomics and Mass Spectrometry Studies
- Toxic Organic Pollutants Impact
- Fluoride Effects and Removal
- Radiomics and Machine Learning in Medical Imaging
- Computational Drug Discovery Methods
- Microbial Community Ecology and Physiology
- Antimicrobial Peptides and Activities
- Health, Environment, Cognitive Aging
- Mental Health Research Topics
- Bioinformatics and Genomic Networks
- Analytical Chemistry and Chromatography
- Streptococcal Infections and Treatments
- Respiratory Support and Mechanisms
- Antimicrobial Resistance in Staphylococcus
- Advanced Chemical Sensor Technologies
- Gene expression and cancer classification
- Protist diversity and phylogeny
- Marine and coastal ecosystems
- Photosynthetic Processes and Mechanisms
University of Tübingen
2023-2024
Max Planck Institute for Biology
2023
German Center for Infection Research
2023
Non-targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a widely used tool for metabolomics analysis, enabling the detection and annotation of small molecules in complex environmental samples. Data-dependent acquisition (DDA) product ion spectra thereby currently one most frequently applied data strategies. The optimization DDA parameters central to ensuring high spectral quality, coverage, number compound annotations. Here, we evaluated influence 10 settings Q Exactive...
Abstract Long-chain fatty acids with antimicrobial properties are abundant on the skin and mucosal surfaces, where they essential to restrict proliferation of opportunistic pathogens such as Staphylococcus aureus . These (AFAs) elicit bacterial adaptation strategies, which have yet be fully elucidated. Characterizing pervasive mechanisms used by S. resist AFAs could open new avenues prevent pathogen colonization. Here, we identify lipase Lip2 a novel resistance factor against AFAs....
Feature-Based Molecular Networking (FBMN) is a popular analysis approach for LC-MS/MS-based non-targeted metabolomics data. While processing LC-MS/MS data through FBMN fairly streamlined, downstream handling and statistical interrogation often key bottleneck. Especially, users new to struggle effectively handle analyze complex matrices. In this protocol, we provide comprehensive guide the of results. We explain structure principles clean-up normalization, as well uni- multivariate...
Dissolved organic matter (DOM) is an ultracomplex mixture that plays a central role in global biogeochemical cycles. Despite its importance, DOM remains poorly understood at the molecular level. Over last decades, significant efforts have been made to decipher chemical composition of by high-resolution mass spectrometry (HR-MS) and liquid chromatography (LC) coupled with tandem (MS/MS). Yet, complexity high degree nonresolved isomers still hamper full structural analysis DOM. To address this...
Non-targeted liquid chromatography tandem mass spectrometry (LC-MS/MS) is a widely used tool for the detection and annotation of small molecules in complex environmental samples. Data Dependent Acquisition (DDA) product ion spectra thereby currently one most frequently applied data acquisition strategies. The optimization DDA parameters central to ensuring high spectral quality, coverage number compound annotations. Here, we evaluated influence 10 settings Q Exactive spectrometer on natural...
Dissolved organic matter (DOM) is one of the most complex chemical mixtures and plays a central role in biogeochemical cycles across our ecosphere. Despite its importance, DOM remains poorly understood at molecular level. Over last decades, significant efforts have been made to decipher composition by high-resolution mass spectrometry (HRMS) liquid chromatography (LC) coupled with tandem (MS/MS). Yet, complexity high degree non-resolved isomers still hamper full structural analysis DOM. To...
Natural competence requires a contractile pilus system. Here, we provide evidence that the biogenesis and natural in cyanobacteria are regulated by second messenger c-di-AMP. Furthermore, show ComFB signaling protein is novel c-di-AMP-receptor protein, widespread bacterial phyla, required for DNA uptake.
Abstract Long-chain fatty acids with antimicrobial properties are abundant on the skin and mucosal surfaces, where they essential to restrict proliferation of opportunistic pathogens such as Staphylococcus aureus . These (AFAs) elicit bacterial adaptation strategies, which have yet be fully elucidated. Characterizing pervasive mechanisms used by S. resist AFAs could open new avenues prevent pathogen colonization. Here, we identify lipase Lip2 a novel resistance factor against AFAs....