A5103000020- Advanced X-ray and CT Imaging
- Cellular transport and secretion
- Laser-Plasma Interactions and Diagnostics
- Lysosomal Storage Disorders Research
- Calcium signaling and nucleotide metabolism
- Laser-induced spectroscopy and plasma
- Advanced X-ray Imaging Techniques
- Parkinson's Disease Mechanisms and Treatments
- High-pressure geophysics and materials
- X-ray Spectroscopy and Fluorescence Analysis
- Ocular and Laser Science Research
- Nuclear Physics and Applications
- Medical Imaging Techniques and Applications
- Magnetic confinement fusion research
- Neurological diseases and metabolism
- Alzheimer's disease research and treatments
Lawrence Livermore National Laboratory
1986-2024
MRC Protein Phosphorylation and Ubiquitylation Unit
2024
University of Dundee
2024
Medical Research Council
2024
Abstract Lysosomes are implicated in a wide spectrum of human diseases including monogenic lysosomal storage disorders (LSDs), age-associated neurodegeneration and cancer. Profiling content using tag-based immunoprecipitation (LysoTagIP) cell animal models allowed major discoveries the field, however studying dysfunction patients remains challenging. Here, we report development “tagless LysoIP method” to enable rapid enrichment lysosomes, via immunoprecipitation, endogenous integral membrane...
Lysosomes are implicated in a wide spectrum of human diseases including monogenic lysosomal storage disorders (LSDs), age-associated neurodegeneration and cancer. Profiling content using tag-based immunoprecipitation (LysoTagIP) cell animal models has substantially moved the field forward, but studying dysfunction patients remains challenging. Here, we report development 'tagless LysoIP' method, designed to enable rapid enrichment lysosomes, via immunoprecipitation, endogenous integral...
To study matter at extreme densities and pressures, we need mega laser facilities such as the National Ignition Facility well creative methods to make observations during timescales of a billionth second. facilitate this, developed platform diagnostic characterize new point-projection radiography configuration using two micro-wires irradiated by short pulse system that provides large field view with up 3.6 ns separation between images. We used tungsten-carbide solid spheres reference objects...
Eight Lawrence Livermore National Laboratory (LLNL) x-ray streak cameras have been characterized for spatial resolution and the line spread function contribution to temporal resolution. The contrast transfer of a gold grid varying frequency was measured each camera. In addition, image intensifier tube normally used LLNL (ITT 4113) separately. Limiting visual system found at 10 pairs/mm (lp/mm) camera photocathode with corresponding 440 μm FWHM. 14 lp/mm photocathode. Comparisons commerical...
ABSTRACT Leucine-rich repeat kinase 2 (LRRK2) inhibition is a promising disease-modifying therapy for LRRK2-associated Parkinson’s disease (L2PD) and idiopathic PD (iPD). Yet, pharmaco-dynamic readouts progression biomarkers modification clinical trials are insufficient. Employing phospho-/proteomic analyses we assessed the impact that LRRK2 activating mutations had in peripheral blood mononuclear cells (PBMCs) from cohort Spain (n=174) encompassing G2019S L2PD patients (n=37),...
Abstract Leucine-rich repeat kinase 2 (LRRK2) inhibition is a promising disease-modifying therapy for LRRK2-associated Parkinson’s disease (L2PD) and idiopathic PD (iPD). However, pharmaco-dynamic readouts progression biomarkers clinical trials aiming modification are insufficient since no endogenous marker reflecting enhanced activity of the most common LRRK2 G2019S mutation has been reported yet in L2PD patients. Employing phospho-/proteomic analyses we assessed impact that activating...