A5042908961- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- Neuroendocrine regulation and behavior
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Cellular transport and secretion
- Hearing, Cochlea, Tinnitus, Genetics
- Lipid Membrane Structure and Behavior
- Molecular Biology Techniques and Applications
- Neuropeptides and Animal Physiology
- Biochemical Analysis and Sensing Techniques
- Alcohol Consumption and Health Effects
- Stress Responses and Cortisol
- Education, Psychology, and Social Research
- Chemical Synthesis and Analysis
- Neurotransmitter Receptor Influence on Behavior
- Advanced Glycation End Products research
- Proteoglycans and glycosaminoglycans research
- Steroid Chemistry and Biochemistry
- Birth, Development, and Health
- Ubiquitin and proteasome pathways
- Sexual Differentiation and Disorders
Czech Academy of Sciences, Institute of Experimental Medicine
2012-2024
Charles University
2023
Czech Academy of Sciences
2008-2023
Czech Academy of Sciences, Institute of Molecular Genetics
2016
Columbia University
2008-2010
GABA
GABA B receptors are the G-protein coupled for main inhibitory neurotransmitter in brain, GABA. were shown to associate with homo-oligomers of auxiliary KCTD8, KCTD12, KCTD12b, and KCTD16 subunits (named after their T1 K + -channel tetramerization domain) that regulate signaling receptor. Here we provide evidence also hetero-oligomers KCTD subunits. Coimmunoprecipitation experiments indicate two-thirds proteins hippocampus adult mice KCTD12. We show hetero-oligomerize through...
The properties of glycine receptors (GlyRs) depend upon their subunit composition. While the prevalent adult forms GlyRs are heteromers, previous reports suggested functional α homomeric in mature nervous tissues. Here we show two functionally different populations rat medial nucleus trapezoid body (MNTB). Postsynaptic formed α1/β-containing clusters on somatodendritic domains MNTB principal neurons, colocalizing with glycinergic nerve endings to mediate fast, phasic IPSCs. In contrast,...
The physiological functions of glycine receptors (GlyRs) depend on their subcellular locations. In axonal terminals the central neurons, GlyRs trigger a slow facilitation presynaptic transmitter release; however, spatial relationship to release sites is not known. this study, we examined distribution in rat glutamatergic calyx Held nerve terminal using high-resolution pre-embedding immunoelectron microscopy. We performed quantitative analysis GlyR-associated immunogold (IG) labeling 3D...
High-frequency oscillations (HFOs) represent an electrographic biomarker of endogenous epileptogenicity and seizure-generating tissue that proved clinically useful in presurgical planning delineating the resection area. In neocortex, clinical observations on HFOs are not sufficiently supported by experimental studies stemming from a lack realistic neocortical epilepsy models could provide explanation pathophysiological substrates HFOs. this study, we explored pathological epileptiform...
Stress responses are activated by the hypothalamic-pituitary-adrenal axis (HPA axis), culminating in release of glucocorticoids. During prolonged periods secretion glucocorticoids or inappropriate behavioral to a stressor, pathologic conditions may occur. Increased glucocorticoid concentration is linked generalized anxiety, and there knowledge gaps regarding its regulation. It known that HPA under GABAergic control, but contribution individual subunits GABA receptor largely unknown. In this...