A5024152241- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Chromatin Dynamics
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Biosimilars and Bioanalytical Methods
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- HER2/EGFR in Cancer Research
- Plant-Microbe Interactions and Immunity
- Enzyme Structure and Function
- Alzheimer's disease research and treatments
- Psoriasis: Treatment and Pathogenesis
- Viral Infectious Diseases and Gene Expression in Insects
- Peptidase Inhibition and Analysis
- Plant and Fungal Interactions Research
- Plant Virus Research Studies
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cancer-related gene regulation
- IL-33, ST2, and ILC Pathways
- CRISPR and Genetic Engineering
Merck Serono (Switzerland)
2010-2016
Scripps Research Institute
2003-2010
University of Fribourg
1994-1999
Kyushu University
1994
In previous studies, we have developed a technology for the rapid construction of novel DNA-binding proteins with potential to recognize any unique site in given genome. This relies on modular assembly modified zinc finger domains, each which recognizes three bp subsite DNA. A complete set 64 domains would provide comprehensive recognition desired DNA sequence, and new could be assembled by laboratory matter hours. However, critical parameter this approach is extent domain functions as an...
Mutations in the unc-51 gene of nematode Caenorhabditis elegans result various abnormalities axonal elongation and structures. We cloned by tagging with transposon Tc1. The wild-type gene, which rescued mutant phenotypes, encodes a novel serine/threonine kinase 856 amino acids. Mutation sites were identified six mutants. A Lys-->Met mutation created vitro domain led to loss rescuing activity was dominant negative, indicating that Unc-51 is essential for function. Expression an unc-51/lacZ...
Biosimilars are biological products that highly similar to existing approved by health authorities. Demonstration of similarity starts with the comprehensive analysis reference product and its proposed biosimilar at physicochemical functional levels. Here, we report results a comparative adalimumab MSB11022 product, Humira®. Three batches up 23 Humira® were analyzed set state-of-the-art orthogonal methods. Primary higher order structure included N/C-terminal modifications, molecular weight...
Artificial transcription factors can be engineered to interact with specific DNA sequences modulate endogenous gene expression within cells. A significant hurdle implementation of this approach is the selection appropriate sequence for targeting. We reasoned that a good target site should located in chromatin, where it accessible DNA-binding proteins, and close vicinity known transcriptional regulators gene. Here we have explored efficacy these criteria guide our potential gamma-globin...
Telomerase is the ribonucleoprotein complex responsible for maintenance of physical ends, or telomeres, most eukaryotic chromosomes. In this study, telomerase activity has been identified in cell extracts from nematode Ascaris suum. This parasitic particularly suited as a model system study telomerase, because it shows phenomenon chromatin diminution, consisting developmentally programmed chromosomal breakage, DNA elimination, and new telomere formation. vitro, A. suum capable efficiently...
Signaling through the ErbB family of tyrosine kinase receptors in normal and cancer-derived cell lines contributes to growth differentiation.In this work, we altered levels ErbB2 ErbB3 receptors, individually combination, by using 6-finger 12-finger synthetic zinc finger protein artificial transcription factors (ATFs) an epidermoid squamous carcinoma line, A431.We successfully designed ATFs capable coregulating ICAM-1 or ErbB3.With ATFs, effects changes receptor were evaluated proliferation,...
Forced degradation, also known as stress testing, is used throughout pharmaceutical development for many purposes including assessing the comparability of biopharmaceutical products according to ICH Guideline Q5E. These formal studies, results which are submitted health authorities, investigate potential impacts manufacturing process changes on quality, safety, and efficacy drug. Despite wide use forced degradation in assessments, detailed guidance design interpretation such studies scarce....