A5025123523- MicroRNA in disease regulation
- Extracellular vesicles in disease
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Nanopore and Nanochannel Transport Studies
- Blood properties and coagulation
- Reproductive System and Pregnancy
- Ovarian cancer diagnosis and treatment
- RNA Interference and Gene Delivery
University of Kansas Medical Center
2023-2025
The human fallopian tube epithelium (hFTE) is the site of fertilization, early embryo development, and origin most high-grade serous ovarian cancers (HGSOCs). Little known about content functions hFTE-derived small extracellular vesicles (sEVs) due to limitations biomaterials proper culture methods. We have established a microfluidic platform hFTE for EV collection with adequate yield mass spectrometry-based proteomic profiling, reported 295 common sEV proteins first time. These are...
The fallopian tube (FT) plays a crucial role in fertility, gynecological health, and high-grade serous ovarian cancer (HGSOC) development. Despite its importance, the spatial transcriptome of FT's distinct anatomical regions (fimbria, infundibulum, ampulla, isthmus) remains underexplored. Using GeoMx Digital Spatial Profiler (DSP) targeted ~1800 gene panel, we analyze premenopausal FT epithelium, identifying region-specific expression patterns. Our analysis reveals upregulation mature...
Abstract Epithelial ovarian cancer (EOC) remains the leading cause of death from gynecological malignancy and fifth all cancer-related deaths among American women. The most common lethal subtype, high-grade serous (HGSOC) tends to originate fallopian tube. Precursor lesions HGSOC such as secretory cell expansion (SCE), loss PAX2, PTEN, p53 mutation tubal intraepithelial carcinoma (STIC) are often found at tube epithelium (FTE). However, mechanism how neoplastic disseminate in FTE eventually...
Extracellular vesicles (EVs) are cell-secreted lipid bilayer delimited particles that mediate cellular communication. These tiny sacs of information play an important role in cell communication and alter the physiological process under both normal pathological conditions. As such, tracking EVs can provide valuable regarding basic understanding communication, onset early malignancy, biomarker discovery. Most current EV-tracking strategies invasive, altering natural characteristics by...