A5008026546- Reproductive Biology and Fertility
- Prenatal Screening and Diagnostics
- Genetic Syndromes and Imprinting
- Effects of Radiation Exposure
- Ovarian function and disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Sperm and Testicular Function
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
Karolinska Institutet
2024
University of Helsinki
2023
University of Tartu
2021-2023
Pregnancy loss is often caused by chromosomal abnormalities of the conceptus. The prevalence these and allocation (ab)normal cells in embryonic placental lineages during intrauterine development remain elusive. In this study, we analyzed 1,745 spontaneous pregnancy losses found that roughly half (50.4%) products conception (POCs) were karyotypically abnormal, with maternal paternal age independently contributing to increased genomic aberration rate. We applied genome haplarithmisis a subset...
STUDY QUESTION: How do human cortical (cPOCs) and medullary (mPOCs) primary ovarian cells respond to acute X-ray exposure? SUMMARY ANSWER: Acute high-dose exposure causes a shift in cPOCs mPOCs transcriptomic profiles impairs significantly their cell-cell adhesion ability. WHAT IS KNOWN ALREADY: Radiotherapy is leading cancer treatment, due its effectiveness targeting malignant cells. However, it can also affect healthy cells, potentially causing organ dysfunction, among which ovaries. When...
Abstract Purpose Ovarian tissue cryopreservation is vital for fertility preservation, yet its effect on ovarian follicle survival and transcriptomic signature requires further investigation. This study delves into the effects of vitrification morphology, function, changes, helping to find possibilities protocol improvements. Methods cortex from 19 bovine animals were used conduct pre- post-vitrification culture followed by histological assessment, immunohistochemistry, TUNEL assay....
Here, we present a modification of single-cell tagged reverse transcription protocol to study gene expression on level or with limited RNA input. We describe different enzymes for and cDNA amplification, modified lysis buffer, additional clean-up steps before amplification. also detail an optimized sequencing method handpicked single cells, tens hundreds as input material mammalian preimplantation development. For complete details the use execution this protocol, please refer Ezer et al.1
Abstract Study question Which are the potential effects of vitrification on ovarian tissue, isolated follicles and short-term cultured follicles? Summary answer Vitrification-warming protocol using ethylene glycol as a cryoprotectant causes no severe damage to tissue or follicles. What is known already Ovarian cryopreservation (OTC) used for fertility preservation (the only possible approach applicable prepubertal girls). To date, more than 200 babies have been born from cryopreserved...
Abstract Study question To investigate the prevalence and effect of (mosaic) de novo genomic aberrations in recurrent pregnancy loss (RPL) sporadic abortion (SA). Summary answer Prevalence maternal uniparental disomies (UPDs) was high both cohorts. While chromosomal UPDs were found cohorts, genome wide RPL specific. What is known already Spontaneous occurs 10–15% clinically recognized pregnancies 1–3%. SA are associated with reduced quality life. Multiple factors contribute to RPL, such as...