A5022410212- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Renal and related cancers
- Chromosomal and Genetic Variations
- Tissue Engineering and Regenerative Medicine
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
Genome Institute of Singapore
2008-2011
Duke-NUS Medical School
2011
National University of Singapore
2006-2010
Mammalian genomes harbor a larger than expected number of complex loci, in which multiple genes are coupled by shared transcribed regions antisense orientation and/or bidirectional core promoters. To determine the incidence, functional significance, and evolutionary context mammalian we identified characterized 5,248 cis–antisense pairs, 1,638 promoters, 1,153 chains bidirectionally promoted pairs from 36,606 mouse transcriptional units (TUs), along with 6,141 2,113 1,480 42,887 human TUs....
The maintenance of pluripotency and specification cellular lineages during embryonic development are controlled by transcriptional regulatory networks, which coordinate specific sets genes through both activation repression. repressor RE1-silencing transcription factor (REST) plays important but distinct roles in (ESC) neural (NSC) stem cells. We investigated how these biological effected at a genomic level. present integrated, comparative genome- transcriptome-wide analyses networks...
Abstract The transcription factor Zic3 is required for maintenance of ESC pluripotency. By genome-wide chromatin immunoprecipitation (ChIP-chip) in ESCs, we have identified 379 direct targets, many which are functionally associated with pluripotency, cell cycle, proliferation, oncogenesis, and early embryogenesis. Through a computational analysis target sequences, novel consensus binding motif (5′-CCC/TGCTGGG-3′). ChIP results vitro DNA assays revealed that binds high affinity specificity on...
Abstract Oct4, Sox2, and Nanog are key components of a core transcriptional regulatory network that controls the ability embryonic stem cells to differentiate into all cell types. Here we show Zfp281, zinc finger transcription factor, is component it required maintain pluripotency. Zfp281 was shown directly activate expression by binding site in promoter very close proximity Oct4 Sox2 sites. We present data showing physically interacts with Nanog. Chromatin immunoprecipitation experiments...
Zfp206 (recently renamed Zscan10) encodes a zinc finger transcription factor specifically expressed in human and mouse embryonic stem cells (ESC). It has been shown that is required to maintain ESC an undifferentiated, pluripotent state. Presented here are data showing works together with two other factors, Oct4 Sox2, which also essential regulators of pluripotency. We show binds the promoter directly regulates expression. Genome-wide mapping Zfp206-binding sites identifies more than 3000...
The differentiation of pluripotent embryonic stem cells is regulated by networks activating and repressing transcription factors that orchestrate determinate patterns gene expression. With the recent mapping target sites for many factors, it has been a conundrum so few genes directly targeted these are transcriptionally responsive to binding factor. To address this, we generated genome-wide maps transcriptional repressor REST five its corepressors in mouse cells. Combining binding-site with...
Abstract Background Transcriptional control of embryonic stem (ES) cell pluripotency has been a subject intense study. regulators including Oct4 (Oct3/4 index), Sox2 and Nanog are fundamental for maintaining the undifferentiated state. However, ES transcriptome is not limited to their targets, exhibits considerable complexity when assayed with microarray, MPSS, cDNA/EST sequencing, SAGE technologies. To identify novel genes associated pluripotency, we globally searched transcripts...