A5040529258- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Seed Germination and Physiology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant and animal studies
- Plant Taxonomy and Phylogenetics
- MicroRNA in disease regulation
- Biocrusts and Microbial Ecology
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
Northeast Forestry University
2022-2025
Competitive endogenous RNAs (ceRNAs) absorb microRNAs and subsequently promote corresponding mRNA long noncoding RNA (lncRNA) expression, which may alter cancer cell malignancy. Thus, dissecting ceRNA networks reveal novel targets in therapies. In this study, we analyzed differentially expressed genes (DEGs) of mRNAs lncRNAs, (DE-miRNAs) circular (DE-circRNAs) extracted from high-throughput sequencing datasets hepatocellular carcinoma patients. Based on these data, identified 26 gene modules...
In this study, the morphological and anatomical characteristics of growth internal ovules dynamic changes in content endogenous hormones during development Korean pine (Pinus koraiensis Siebold & Zucc.) cones were investigated detail their interrelationships determined. addition, examinations, paraffin section, analysis enzyme immunoassays performed to observe as well fertilization stages P. from July pollination year June following year. From May next year, increase indoleacetic acid...
During somatic embryogenesis of Pinus koraiensis, the ability embryogenic callus to produce embryos gradually decreases with long-term proliferative culture, which seriously affects large-scale application this technology. In study, calli Korean pine 1–100 cell line were used as materials. It was found that in 7-day subculture cycle potential remained for longer time. comparison a 14 days, indoleacetic acid (IAA), soluble sugar, and starch contents higher 7 while hydrogen peroxide (H2O2)...
The fertilization process is a critical step in plant reproduction. However, the mechanism of action and mode regulation gymnosperms remain unclear. In this study, we investigated molecular regulatory networks involved Korean pine ovules through anatomical observation, physiological biochemical assays, transcriptome sequencing technology. morphological results indicated that proceeds demise proteinaceous vacuole, egg cell division, pollen tube elongation. Auxin, cytokinin, soluble sugar,...
Cryopreservation is an important method for the excellent long-term preservation of plant germplasm. This study explores optimal cryopreservation technology embryogenic callus Fraxinus mandshurica to effectively maintain its genetic stability and morphogenesis potential. The conditions were assessed using F. as material, slow cooling was optimized cryopreservation. results indicated that preculture in 0.4 mol·L−1 sorbitol solution 20 h at room temperature, followed by cryoprotection 7.5%...
In order to simplify the experimental procedure and treatment procedure, we preserved embryonic callus (EC) of Fraxinus mandshurica more efficiently. this paper, established a method for cryopreservation EC F. by vitrification. was subcultured 7–10 days (d). Vigorous with good growth conditions were selected, performed The best pre-culture on 0.5 mol·L−1 sucrose medium 3 d, load culture in liquid woody plant (WPM) supplemented 2 glycerol 0.4 60 min, then dehydrate mL vitrification solution...