A5103002301- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Biochemical Analysis and Sensing Techniques
- melanin and skin pigmentation
- Regulation of Appetite and Obesity
- Cancer Genomics and Diagnostics
ETH Zurich
2024
The Francis Crick Institute
2021
It is estimated that only 0.02% of disseminated tumour cells are able to seed overt metastases
The only proteins known to be modified by O-linked lipidation are Wnts and ghrelin, enzymatic removal of this post-translational modification inhibits ligand activity. Indeed, the Wnt-deacylase activity Notum is basis its ability act as a feedback inhibitor Wnt signalling. Whether also deacylates ghrelin has not been determined. We used mass spectrometry assay deacylation co-crystallisation reveal enzyme-substrate interactions at atomic level. CRISPR/Cas technology was tag endogenous assess...