A5092902463- Herpesvirus Infections and Treatments
- Innovative Microfluidic and Catalytic Techniques Innovation
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Toxin Mechanisms and Immunotoxins
- Poxvirus research and outbreaks
- Parvovirus B19 Infection Studies
- Metabolomics and Mass Spectrometry Studies
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Liver Disease Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Virology and Viral Diseases
Montana State University
2023-2025
Single-cell analyses of viral infections reveal heterogeneity that is not detected by traditional population-level studies. This study applies drop-based microfluidics to investigate the dynamics herpes simplex virus type 1 (HSV-1) infection neurons at single-cell level. We used micrometer-scale Matrigel beads, termed microgels, culture individual murine superior cervical ganglia (SCG) or epithelial cells. Microgel-cultured cells are encapsulated in media-in-oil droplets with a...
The cell that a virus replicates in i.e., the producer cell, can alter macromolecular composition and infectious capacity of virions are produced. Herpes Simplex type 1 (HSV-1) primarily infects keratinocytes epidermis or oral mucosa prior to establishing latency neurons peripheral nervous system, where persist for lifetime host. Many lines used amplify HSV-1 derived from species tissue types less physiologically relevant disease. To understand if influences infection, we tested infectivity...
Viral infections induce major shifts in cellular metabolism elicited by active viral replication and antiviral responses. For the virus, harnessing evading changes that limit are essential for productive replication. In contrast, response to infection disrupts metabolic pathways prevent promote an state host cell neighboring bystander cells. This competition between virus results measurable differ depending on type, extracellular environment. The resulting can be observed analyzed using...
Critical stages of lytic herpes simplex virus type 1 (HSV-1) replication are marked by the sequential expression immediate early (IE) to (E), then late (L) viral genes. HSV-1 can also persist in neuronal cells via a non-replicative, transcriptionally repressed infection called latency. The regulation and latent transcriptional profiles is critical pathogenesis persistence. We sought fluorescence-based approach observe outcome at single-cell level. To achieve this goal, we constructed...
Subacute sclerosing panencephalitis (SSPE) is a lethal neurological disorder occurring several years after measles. Reconstruction of the evolution measles virus (MeV) genome in an SSPE case suggested that matrix (M) protein mutation M-F50S, when added to other mutations, drove neuropathogenesis. However, whether and how M-F50S would promote spread independently from mutations was question. We investigated here cell specificity MeV this brain documented both neurons astrocytes were heavily...
Abstract Critical stages of lytic Herpes simplex type 1 (HSV-1) replication are marked by the sequential expression immediate early (IE) to (E), then late (L) viral genes. HSV-1 also persists in neuronal tissues via a non-replicative, transcriptionally repressed infection called latency. Understanding regulation and latent transcriptional profiles provides focused insight into disease. We sought fluorescence-based approach observe temporal progression at single-cell level. constructed...
Single-cell analyses of viral infections often reveal heterogeneity that is not detected by traditional population-level studies. This study applies drop-based microfluidics to investigate the dynamics HSV-1 infection neurons at single-cell level. We used micron-scale Matrigel beads, termed microgels, culture individual murine Superior Cervical ganglia (SCG) or epithelial cells. Microgel-cultured cells are subsequently enclosed in media-in-oil droplets with a dual fluorescent-reporter HSV-1,...