A5035188295- interferon and immune responses
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
Institute of Medical Microbiology and Hygiene
2023
Most SARS-CoV-2 proteins are translated from subgenomic RNAs (sgRNAs). While the majority of these sgRNAs monocistronic, some viral mRNAs encode more than one protein. One example is ORF3a sgRNA that also encodes ORF3c, an enigmatic 41-amino-acid peptide. Here, we show ORF3c expressed in SARS-CoV-2-infected cells and suppresses RIG-I- MDA5-mediated IFN-β induction. interacts with signaling adaptor MAVS, induces its C-terminal cleavage, inhibits interaction RIG-I MAVS. The immunosuppressive...
Accumulating evidence suggests that endogenous retroviruses (ERVs) play an important role in the host response to infection and development of disease. By analyzing ChIP-sequencing data sets, we show SARS-CoV-2 induces H3K27 acetylation several loci within LTR69 subfamily ERVs. Using functional assays, identified one SARS-CoV-2-activated locus, termed Dup69, which exhibits regulatory activity is responsive transcription factors IRF3 p65/RELA. LTR69_Dup69 located about 500 bp upstream a long...
Abstract Accumulating evidence suggests that endogenous retroviruses (ERVs) play an important role in the host response to infection and development of disease. By combining RNA- ChIP-sequencing analyses with RT-qPCR, we show SARS-CoV-2 induces LTR69 subfamily ERVs, both vitro vivo . Using functional assays, identified one SARS-CoV-2-activated locus, termed Dup69, which exhibits enhancer activity is responsive transcription factors IRF3 p65/RelA. LTR69-Dup69 located about 500 bp upstream a...