A5016041982- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Additive Manufacturing and 3D Printing Technologies
- Liver physiology and pathology
- Electrospun Nanofibers in Biomedical Applications
- Osteoarthritis Treatment and Mechanisms
- Nanofabrication and Lithography Techniques
- Periodontal Regeneration and Treatments
- CAR-T cell therapy research
- Cellular Mechanics and Interactions
- Advanced Materials and Mechanics
Utrecht University
2021-2025
Organ- and tissue-level biological functions are intimately linked to microscale cell-cell interactions the overarching tissue architecture. Together, biofabrication organoid technologies offer unique potential engineer multi-scale living constructs, with cellular microenvironments formed by stem cell self-assembled structures embedded in customizable bioprinted geometries. This study introduces volumetric bioprinting of complex organoid-laden which capture key human liver. Volumetric via...
Biofabrication via light-based 3D printing offers superior resolution and ability to generate free-form architectures, compared conventional extrusion technologies. While extensive efforts in the design of new hydrogel bioinks lead major advances methods, accessibility lithographic bioprinting is still hampered by a limited choice cell-friendly resins. Herein, we report development novel set photoresponsive bioresins derived from ichthyic-origin gelatin, designed print high-resolution...
Conventional additive manufacturing and biofabrication techniques are unable to edit the chemicophysical properties of printed object postprinting. Herein, a new approach is presented, leveraging light-based volumetric printing as tool spatially pattern any biomolecule interest in custom-designed geometries even across large, centimeter-scale hydrogels. As biomaterial platform, gelatin norbornene resin developed with tunable mechanical suitable for tissue engineering applications. The can be...
There is an increasing need for novel biomaterials compatible with advanced biofabrication technologies, which also permit cells to remodel their microenvironment. This remodelling crucial maturing tissue constructs into fully functional replacements. Recent progress in supramolecular chemistries has allowed the production of dynamic biomaterials. Their properties enable bonds be reversibly broken by cells, facilitating processes requiring morphological changes or migration, development and...
The use of acellular hydrogels to repair osteochondral defects requires cells first invade the biomaterial and then deposit extracellular matrix for tissue regeneration. Due diverse physicochemical properties engineered hydrogels, specific that allow or even improve behaviour are not yet clear. aim this study was investigate influence various on cell migration related formation using in vitro, ex vivo models.Three hydrogel platforms were used study: Gelatine methacryloyl (GelMA) (5% wt),...
Abstract Fabricating hydrogel‐based channels with diameters below 200 µm remains challenging in advanced vitro modeling and tissue engineering. To address this challenge, thermoshrinkable hydrogels that undergo reversible isotropic dimensional changes temperature are developed. A thermoresponsive polymer methacrylate groups (PNH‐MA) is synthesized from polyethylene glycol (PEG), N–isopropylacrylamide (NIPAM), 2‐hydroxyethyl acrylate (HEA), enabling photo‐cross‐linking precise material...
Volumetric Bioprinting Tomographic light fields enable the photopatterning of bioactive proteins and morphogens in 3D, across centimeter-scale, printed hydrogel construct. In article number 2300026, Riccardo Levato co-workers report development volumetric bioprinting, not only as a manufacturing technology, but also to draw imprint gradients patterns growth factors any custom-designed geometry. This technology has implication tissue engineering, regenerative medicine, developmental biology...
Volumetric Bioprinting bioprinting shapes organoid-laden constructs into centimeter-scale assemblies that mimic native liver function. In article number 2110054, Riccardo Levato and co-workers report the development of a hydrogel-based bioresin with tunable optical properties to minimize scattering in light-based printing ensure high resolution. Organoid viability maturation is preserved by shear-stress-free printing, salient functions mature response 3D bioprinted architecture.
Abstract Background Hydrogel-based 3D culture systems are emerging as a valuable tool for preclinical screening of cell-based immunotherapies against solid and hematological malignancies, such chimeric antigen receptor T (CAR-T) cells. Hydrogels can influence cell function in non-desired manner due to their mechanical properties chemical composition, potentially skewing results testing novel immunotherapeutic compounds. Methods In this study, we assess CD4 + CAR-T activation proliferation...