A5108058855- Advanced Electron Microscopy Techniques and Applications
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Force Microscopy Techniques and Applications
- Lipid metabolism and biosynthesis
- Immune cells in cancer
- Advanced Memory and Neural Computing
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Natural product bioactivities and synthesis
- Bioactive natural compounds
- Machine Learning in Materials Science
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- NF-κB Signaling Pathways
University Medical Center
2024-2025
Universitätsmedizin Göttingen
2022-2024
University of Göttingen
2022-2024
Institute of Mathematics
2004
Abstract Despite decades of intense research, the molecular organization synapse is not well understood. To address this issue, we sought to develop a method for systematic imaging synapses by cryo-electron tomography (cryo-ET), technology capable mapping cellular architecture at resolution. Thinning samples cryo-focused ion beam milling prerequisite high-quality cryo-ET imaging, but process needs be guided structures interest. allow robust synaptic targeting, established correlative...
Abstract Electron microscopy is an important technique for the study of synaptic morphology and its relation to function. The data analysis this task requires segmentation relevant structures, such as vesicles, active zones, mitochondria, presynaptic densities, ribbons, compartments. Previous studies were predominantly based on manual segmentation, which very time-consuming prevented systematic large datasets. Here, we introduce SynapseNet, a tool automatic synapses in electron micrographs....
Abstract For see ChemInform in Full Text.
Abstract Crucial brain functions such as neurotransmission, myelination, and signaling pose a high demand for lipids. Lipid dysregulation is associated with neuroinflammation neurodegeneration. Astrocytes protect neurons from lipid induced damage by accumulating metabolizing toxic lipids in organelles called droplets (LDs). LDs have long been considered storage compartments adipocytes, but less known about their biogenesis composition the brain. In particular, proteins covering LD surface...
Mouse bone marrow-derived macrophages (BMDM) have been identified as an important host cell model for studying mammalian macrophage functions during pathogen infection. Whereas colony stimulating factor (CSF)-1 is required BMDM differentiation, the commercial CSF-1, however, expensive. Therefore, L929 cell-conditioned medium (LCM) has reported to be used a source of CSF-1 in many recent studies. However, whether differentiated by and LCM had any functional differences bacterial infection,...
Abstract Crucial brain functions such as neurotransmission, myelination, and signaling pose a high demand for lipids. Lipid dysregulation is associated with neuroinflammation neurodegeneration. Astrocytes protect neurons from lipid induced damage by accumulating metabolizing toxic lipids in organelles called droplets (LDs). LDs have long been considered storage compartments adipocytes, but less known about their biogenesis composition the brain. In particular, proteins covering LD surface...