A5048006119- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Physiological and biochemical adaptations
- Cancer-related gene regulation
- Insect and Arachnid Ecology and Behavior
- Parasite Biology and Host Interactions
- Neurobiology and Insect Physiology Research
- Animal Behavior and Reproduction
- Insect-Plant Interactions and Control
- Genetics and Neurodevelopmental Disorders
- Chromosomal and Genetic Variations
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
University of North Carolina at Chapel Hill
2019-2024
Abstract Insect parasitoids, and the arthropod hosts they consume during development, are important ecological players in almost all environments across globe. As ectothermic organisms, both parasitoid host strongly impacted by environmental temperature. If thermal tolerances differ between insect parasitoid, then outcome of their interaction will be determined ambient mean temperatures continue to rise extreme become more frequent, we must determine effect high temperature stress on...
Monomethylation of lysine 20 histone H4 (H4K20me1) is catalyzed by Set8 and thought to play important roles in many aspects genome function that are mediated H4K20me binding proteins. We interrogated this model a developing animal comparing parallel the transcriptomes null , K20R/A l(3)mbt mutant Drosophila melanogaster . found gene expression profiles K20A K20R larvae markedly different than despite similar reductions H4K20me1. cells have severely disrupted transcriptome fail proliferate...
When thermal tolerances differ between interacting species, extreme temperature events (heat waves) will alter the ecological outcomes. The parasitoid wasp
Abstract Mono-methylation of Lysine 20 histone H4 (H4K20me1) is catalyzed by Set8 and thought to play important roles in many aspects genome function that are mediated H4K20me-binding proteins. We interrogated this model a developing animal comparing parallel the transcriptomes null , K20R/A l(3)mbt mutant Drosophila melanogaster . found gene expression profiles K20A K20R larvae markedly different than despite similar reductions H4K20me1. cells have severely disrupted transcriptome fail...
ABSTRACT Climate change is increasing the frequency of heat waves and other extreme weather events experienced by organisms. How does number developmental timing affect survival, growth development insects? Do early in alter performance later development? We addressed these questions using experimental with larvae tobacco hornworm, Manduca sexta. The experiments used diurnally fluctuating temperature treatments differing (0–3) (early, middle and/or late larval development) waves, which a...
Replication initiation in eukaryotic cells occurs asynchronously throughout S phase, yielding early- and late-replicating regions of the genome, a process known as replication timing (RT). RT changes during development to ensure accurate genome duplication maintain stability. To understand relative contributions that cell lineage, cycle, regulators have on RT, we utilized powerful developmental systems available
Abstract Diurnal fluctuations in temperature are ubiquitous terrestrial environments, and insects other ectotherms have evolved to tolerate or acclimate such fluctuations. Few studies examined whether diurnal fluctuations, how natural domesticated populations differ their responses We examine diurnally fluctuating temperatures during development affect growth, acclimation, stress for two of Manduca sexta : a field population that typically experiences wide variation mean temperature,...
Asynchronous replication of chromosome domains during S phase is essential for eukaryotic genome function, but the mechanisms establishing which replicate early versus late in different cell types remain incompletely understood. Intercalary heterochromatin very both diploid chromosomes dividing cells and endoreplicating polytene where they are also underreplicated. Drosophila SNF2-related factor SUUR imparts locus-specific underreplication chromosomes. negatively regulates DNA fork...
Abstract Replication initiation in eukaryotic cells occurs asynchronously throughout S phase, yielding early and late replicating regions of the genome, a process known as replication timing (RT). RT changes during development to ensure accurate genome duplication maintain stability. To understand relative contributions that cell lineage, cycle, regulators have on RT, we utilized powerful developmental systems available Drosophila melanogaster . We generated compared profiles from mitotic...
Abstract Asynchronous replication of chromosome domains during S phase is essential for eukaryotic genome function, but the mechanisms establishing which replicate early versus late in different cell types remain incompletely understood. Drosophila SNF2-related factor SUUR imparts under- late-replicating intercalary heterochromatin polytene chromosomes. negatively regulates DNA fork progression; however, its mechanism action remains obscure. Here we developed a novel method termed MS-Enabled...