A5057771335- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Photosynthetic Processes and Mechanisms
- Click Chemistry and Applications
- RNA modifications and cancer
- Biotin and Related Studies
- Genetics, Aging, and Longevity in Model Organisms
- Electromagnetic Compatibility and Noise Suppression
- Electromagnetic Compatibility and Measurements
- Electrostatic Discharge in Electronics
- Genomics and Chromatin Dynamics
- Smart Parking Systems Research
- Indoor and Outdoor Localization Technologies
- RNA and protein synthesis mechanisms
- Inertial Sensor and Navigation
University of Southern California
2023
Stanford University
2020
PES University
2019
The University of Texas at Dallas
2019
Significance Most of the thousands proteins that comprise a human cell have specific subcellular localization patterns essential for their function. “Proximity labeling” (PL) is method mapping endogenous cellular on proteome-wide scale. To improve specificity and versatility PL, we developed split-TurboID, promiscuous biotinylating enzyme split into two inactive fragments. The fragments are coexpressed in cells brought together by drug, protein–protein interaction, or organelle contact to...
ABSTRACT RNA silencing pathways are complex, highly conserved, and perform crucial regulatory roles. In Caenorhabditis elegans germlines, surveillance occurs through a series of perinuclear germ granule compartments – P granules, Z SIMR foci, Mutator foci multiple which form via phase separation. Although the functions individual proteins have been extensively studied, relationships between (collectively, ‘nuage’) less understood. We find that key assemble into separate but adjacent...
In the Caenorhabditis elegans germline, fem-3 Binding Factor (FBF) partners with LST-1 to maintain stem cells. A crystal structure of an FBF-2/LST-1/RNA complex revealed that FBF-2 recognizes a short RNA motif different from characteristic 9-nt FBF binding element, and compact recognition coincided curvature changes in scaffold. Previously, we engineered favor shorter motifs without change (Bhat et al., 2019). vitro selection RNAs bound by suggested sequence specificity central region...
Abstract Proximity labeling (PL) catalyzed by promiscuous enzymes such as TurboID have enabled the proteomic analysis of subcellular regions difficult or impossible to access conventional fractionation-based approaches. Yet some cellular regions, organelle contact sites, remain out reach for current PL methods. To address this limitation, we split enzyme into two inactive fragments that recombine when driven together a protein-protein interaction membrane-membrane apposition. At endoplasmic...
Electromagnetic Interference contributes to a significant percentage of failures in the verification stage automotive electronics design cycle. Today these issues are caught late cycle leading delay time-to-market. An effective simulation methodology is key catching problems at an early stage. However, accurate system-level analysis time intensive. This letter presents model-based for immunity characterization The framework comprised hybrid 2D-3D electromagnetic solver and circuit solver. IC...
ABSTRACT RNA silencing pathways are complex, highly conserved, and perform widespread, critical regulatory roles. In C. elegans germlines, surveillance occurs through a series of perinuclear germ granule compartments—P granules, Z SIMR foci, Mutator foci—multiple which form via phase separation exhibit liquid-like properties. The functions individual proteins within granules well-studied, but the spatial organization, physical interaction, coordination biomolecule exchange between...
The advent of smart wearable technology in recent years has seen applications which bridge the gap between diverse aspects day-to-day life. Navigation as one its feature, while providing convenience to a large degree, reputation for quick battery drainage. This paper proposes alleviate this issue with simplistic approach navigation on wearable. proposed method also aims provide real-time orientation based directions user compass-like feature. It accomplishes these functionalities by relying...