A5092014948- Viral Infectious Diseases and Gene Expression in Insects
- Protein Structure and Dynamics
- Nerve injury and regeneration
- Microbial Metabolic Engineering and Bioproduction
- RNA Interference and Gene Delivery
Dompé (Italy)
2023-2024
Fermentation is a widely used process in the biotechnology industry, which sugar-based substrates are transformed into new product through chemical reactions carried out by microorganisms. yields depend heavily on critical parameter (CPP) values need to be finely tuned throughout process; this usually performed biotech production expert relying empirical rules and personal experience. Although developing mathematical model analytically describe how CPP too challenging because involves living...
Thus far, no manufacturing process able to support industrialization has been reported for the recombinant human brain-derived neurotrophic factor (rhBDNF). Here, we described setup of a new protocol its production in Escherichia coli (E. coli) and purification homogeneity. A synthetic gene, codifying neurotrophin precursor, was inserted into an E. expression vector transformed BL21 (DE3) strain. The protein accumulates, at high yields, inclusion bodies. With developed strategy, more than...