A5002699251- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Fibroblast Growth Factor Research
- PI3K/AKT/mTOR signaling in cancer
- Kruppel-like factors research
- Gastrointestinal Tumor Research and Treatment
- Helicobacter pylori-related gastroenterology studies
- Cancer Genomics and Diagnostics
- Virus-based gene therapy research
- Lymphoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Metastasis and carcinoma case studies
- Lung Cancer Treatments and Mutations
- Gastric Cancer Management and Outcomes
- Synthesis and Biological Evaluation
- Circular RNAs in diseases
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetic factors in colorectal cancer
- Layered Double Hydroxides Synthesis and Applications
- Bacterial biofilms and quorum sensing
- Adsorption and biosorption for pollutant removal
- Muscle Physiology and Disorders
- Corneal Surgery and Treatments
- Heat shock proteins research
Tessa Therapeutic (Singapore)
2022-2024
Universidad de Zamboanga
2024
National University of Singapore
2014-2018
National University Cancer Institute, Singapore
2014-2017
Huaian First People’s Hospital
2017
Nanjing Medical University
2017
Central South University
2014-2016
University of Malaya
2013-2016
Staphylococcus aureus is one of the leading causes food poisoning. Its pathogenicity results from possession virulence genes that produce different toxins which result in self-limiting to severe illness often requiring hospitalization. In this study 200 sushi and sashimi samples, S. contamination was confirmed 26% samples. The isolates were further characterized for antibiotic susceptibility. A high incidence identified 96.2% 20 gene profiles confirmed. DNA amplification showed 30.8% (16/52)...
Preclinical models of diffuse-type gastric cancer (DGC) that reliably predict clinical activity novel compounds are lacking. To overcome the problem poor tumor cellularity in DGC, we used cells from malignant ascites to establish DGC patient-derived xenograft (PDX) recapitulate primary cancer. Cells PDX model GAGA6 with FGFR2 amplification were sensitive AZD4547, a potent FGFR inhibitor is being clinically evaluated for FGFR-aberrant types. Intermittent vivo treatment tumors AZD4547 gave...
For the first time, dramatically decreased Dp71 protein and mRNA was found in 34 pairs of resected primary gastric adenocarcinoma. Immunohistochemistry identified expression suppressed 72.2% 104 cancer patients. The significantly correlated with differentiation (P=0.001) lymph vascular invasion (p=0.041). Decreased associated a poor adenocarcinoma prognosis (P=0.001). Significantly less were BGC823, SGC7901, AGS compared GES-1. Via increasing lamin B1 protein, enforced Dp71d Dp71f resulted...
Heat shock factor 1 (HSF1) is one of the most important transcriptional molecules in heat process; however, HSF1 can also regulate expression other proteins. Dystrophin Dp71 widely expressed isoforms dystrophin gene family. In our experiments, we showed for first time that function as a endogenous vivo and vitro. We demonstrated messenger RNA (mRNA) protein were significantly reduced HSF1-knockout mice compared with wild-type brain, lung, liver, spleen, kidney. Overexpression enhanced mRNA...
Abstract Encouraged by the observations of significant B7-H3 protein overexpression in many human solid tumors compared to healthy tissues, we directed our focus towards targeting using chimeric antigen receptor (CAR) T cells. We utilized a nanobody as B7-H3–targeting domain CAR construct circumvent stability issues associated with single-chain variable fragment–based domains. In efforts expand patient access T-cell therapy, engineered nanobody-based into Epstein-Barr virus–specific cells...
Aims: Follicular lymphoma is frequently associated with t(14;18)(q32;q21) translocation. This study was undertaken to determine the pattern of Bcl‐2, CD10 and Bcl‐6 expression in relation t(14;18) translocation follicular from a cohort multi‐ethnic Asian population. Methods results: Sixty‐two cases were retrieved for immunohistochemistry, analysis by polymerase chain reaction fluorescent in‐situ hybridization techniques. Bcl‐2 present 74% cases. also relatively low (61%), decreasing...
β-Thalassemia is a public health problem where 4.5% of Malaysians are β-thalassemia carriers. The genetic disorder caused by defects in the β-globin gene complex which lead to reduced or complete absence chain synthesis. Five TaqMan genotyping assays were designed and developed detect common mutations Malaysian Malays. evaluated with 219 "blinded" DNA samples results showed 100% sensitivity specificity. in-house found be cost- time-effective for characterization population.
Stomach adenocarcinoma is estimated to cause 10,000 deaths in the US 2016 and third most deadly cancer China. We aim identify proteins genes that have impact on prognosis of patients with stomach adenocarcinoma. Data were retrieved from The Cancer Genome Atlas (TCGA). Proteins whose expression levels highly correlated survival status figured out. their mRNAs roles pathway used determine driver gene candidates. effects mutations encoding KIT mRNA expressions carried. Ten antibodies out...
Keratoconus is an ocular degeneration characterized by the thinning of corneal stroma that may lead to varying degrees myopia and visual impairment. Genetic factors have been reported in pathology keratoconus where Asians a higher incidence, earlier onset, undergo grafts compared Caucasians. The system homeobox 1 (VSX1) gene forms part paired-like homeodomain transcription factor which responsible for development. was marked as candidate genetic studies various populations. Single nucleotide...
Abstract In contrast to its limited expression in normal tissues, the B7 homolog 3 protein (B7-H3) is over-expressed many human tumors, making it an attractive target for chimeric antigen receptor (CAR) T cell therapy. To address technical challenges limiting broader patient access autologous CAR therapy, we armoured Epstein-Barr Virus Specific Cells (EBVSTs) with CARs as off-the-shelf therapy against solid tumors. We identified and utilised a nanobody domain specifically targeting B7-H3 our...
<p>Supplementary figure 3</p>
<p>B7-H3.mCAR T cells exhibit little on-tumor off target toxicity in a syngeneic mouse model. <b>A,</b> Body weight (left), tumor volume measurements (middle), and fold change volumes (right) of B16F10-hB7H3 tumors after treatment. <b>B,</b> Endpoint murine immune cell counts blood, bone marrow, spleen, liver quantified by flow cytometry. Each point on the graph represents mouse. <b>C,</b> Pathologic evaluation organs from two B16F10WT-hB7H3...
<p>B7H3.CAR EBVSTs targets allogeneic monocytes and matured HSPCs but spares T, B, NK cells as well resting early HSPCs. <b>A,</b> Cytolysis of allogenic NK, B by UT B7H3.CAR in the presence various cytokines. Fold change cell counts was calculated normalizing immune against that EBVSTs. <b>B,</b> Percentage death after 24 hours coculture at a 1:1 E:T ratio with or <b>C,</b> B7-H3 expression on memory have been reactivated antigen-presenting pulsed...
<p>B7-H3 expression in tumors is higher compared with healthy tissues. <b>A,</b> Grading and pattern of B7-H3 IHC staining human normal <b>B,</b> Level area gastric cancer tumor bed adjacent areas. <b>C,</b> stromal. <b>D,</b> breast <b>E,</b> TNBC. <b>F,</b> H-score for versus For F, groups were using Student unpaired <i>t</i> test. ****, <i>P</i> < 0.0001. Each ID or point on the graph...
<p>Supplementary table 1</p>
<p>Supplementary table 1</p>
<p>Supplementary figure 7</p>
<p>Supplementary figure 8</p>
<p>Supplementary Material and Methods</p>
<p>Supplementary figure 2</p>
<p><i>In vivo</i> targeting of allogeneic myeloid cells by B7H3.CAR EBVSTs is linked to a lower risk CRS. <b>A,</b> IVIS monitoring was performed track tumor progression. Bioluminescence images burden in mice injected intravenously with NALM-6 (left) or HT-29 (right) at days −1, 2, and 5 posttreatment. <b>B,</b> Average represented each treatment group (top) (bottom). Body weight measurements (<b>C</b>) HT-29-injected...
<p>Supplementary figure 1</p>