A5103058909- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Genetic and phenotypic traits in livestock
- Identification and Quantification in Food
- Metallurgy and Material Forming
- Animal Diversity and Health Studies
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- RNA Interference and Gene Delivery
The University of Tokyo
2023-2024
Each nucleosome contains four types of histone proteins, each with a tail. These tails are essential for the epigenetic regulation gene expression through post-translational modifications (PTMs). However, their influence on dynamics at single-molecule level remains undetermined. Here, we employed high-speed atomic force microscopy to visualize in absence N-terminal tail or all tails. Loss stripped 6.7 base pairs from core, and DNA entry-exit angle expanded by 18° that wild-type nucleosomes....
The N-terminal tails of histones protrude from the nucleosome core and are target sites for histone modifications, such as acetylation methylation. Histone is considered to enhance transcription in chromatin. However, contribution tail by RNA polymerase II (RNAPII) has not been clarified. In present study, we reconstituted nucleosomes lacking each histone, H2A, H2B, H3 or H4, performed RNAPII assays. We found that H3, but H2B functions pausing at SHL(-5) position nucleosome. Consistently,...