A5101543322- Genetics, Aging, and Longevity in Model Organisms
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Spaceflight effects on biology
- Circadian rhythm and melatonin
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Light effects on plants
Rowan University
2021-2024
Drexel University
1999
The auxin-inducible degradation system in C. elegans allows for spatial and temporal control of protein via heterologous expression a single Arabidopsis thaliana F-box protein, transport inhibitor response 1 (AtTIR1). In this system, exogenous auxin (Indole-3-acetic acid; IAA) enhances the ability AtTIR1 to function as substrate recognition component that adapts engineered degron-tagged proteins endogenous E3 ubiquitin ligases complex [SKR-1/2-CUL-1-F-box (SCF)], targeting them by...
Abstract The heterochronic genes of Caenorhabditis elegans comprise the best-studied pathway controlling timing tissue and organ formation in an animal. To begin to understand evolution this significance relationships among its components, we characterized 11 briggsae orthologs C. genes. Using CRISPR/Cas9, made a variety alleles found that several mutant phenotypes differ significant ways from those elegans. Although most displayed defects developmental timing, their could which stages were...
Abstract The heterochronic genes of the nematode Caenorhabditis elegans control succession postembryonic developmental events. 4 core lin-14, lin-28, hbl-1, and lin-41 act in a sequence to specify cell fates specific each larval stages. It was previously shown that lin-14 has 2 activities separated time promote L1 L2 events, respectively. Using auxin-inducible degron system, we find lin-28 hbl-1 have L3 events which are also time. Relative they control, both appear just prior or concurrently...
Abstract The heterochronic genes of the nematode Caenorhabditis elegans control succession postembryonic developmental events. four core lin-14, lin-28, hbl-1, and lin-41 act in a sequence to specify cell fates specific each larval stages. It was previously shown that lin-14 has two activities separated time promote L1 L2 events, respectively. Using auxin-inducible degron system, we find lin-28 hbl-1 have L3 events which are also time. Relative they control, both appear just prior or...
Abstract Characteristically, the heterochronic genes control stage-specific timing of developmental events throughout developing Caenorhaditis elegans larva while gonad development occurs mostly normally. In a few cases, some regulators lead to detectable variations in with little no effect on fertility. We found that double mutant lin-28 null allele and hbl-1 hypomorphic results catastrophic failure morphogenesis resulting sterility. This defect includes high-penetrance disruption normal...
Abstract The heterochronic genes of C. elegans comprise the best-studied pathway controlling timing tissue and organ formation in an animal. To begin to understand evolution this significance relationships among its components, we characterized 11 briggsae orthologs genes. Using CRISPR/Cas9, made a variety alleles found that several mutant phenotypes differ significant ways from those . Although most displayed defects developmental timing, their could which stages were affected, penetrance...
The heterochronic genes of C. elegans comprise the best-studied pathway controlling timing tissue and organ formation in an animal. To begin to understand evolution this pathway, significance each factor, relationships among components, we characterized 11 briggsae orthologs genes. Using CRISPR/Cas9, made a variety alleles found that several mutant phenotypes differ significant ways from those elegans. Although most displayed defects developmental timing, could which stages they controlled,...