A5102882417- Reproductive Physiology in Livestock
- Ruminant Nutrition and Digestive Physiology
- Animal health and immunology
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Effects of Environmental Stressors on Livestock
- Metabolism and Genetic Disorders
- Meat and Animal Product Quality
- Genetic and phenotypic traits in livestock
- Muscle metabolism and nutrition
- Fatty Acid Research and Health
Purdue University West Lafayette
2024-2025
Periparturient dairy cattle undergo physiological adaptations to support fetal growth and colostrum synthesis in late gestation milk production early lactation. To energy protein demands mobilize body tissue reserves. The objective of this study was determine the effects prepartum skeletal muscle reserves supplementation branched-chain volatile fatty acids (BCVFA) on composition measurements, metabolic markers related health, protein, status, subsequent yield multiparous cows. Skeletal were...
Pyruvate carboxylase (PC) catalyzes the formation of oxaloacetate, a TCA cycle intermediate and gluconeogenic substrate. Altering saturated to unsaturated fatty acid ratio alters PC expression, suggesting central role in mediating carbon flow through metabolic pathways. Herein, we describe changes flux intermediates proteome Madin Darby bovine kidney (MDBK) cells with expression knocked-down (PC-KD), overexpressed (PC-OE), unaltered using Scramble control, or pretreated for 21 h vehicle...
Dairy cattle mobilize skeletal muscle in the periparturient period to close energy and metabolizable protein gaps due high demands of growing fetus milk production. The objective this study was determine if amount dam prepartum reserves branched-chain VFA (BCVFA) supplementation affected calf birth weight, circulating AA, semitendinosus metabolic activity, colostrum composition. We hypothesized that calves born dams with higher supplemented BCVFA would be heavier, have greater mass as...
Nutrition and physiological state affect hepatic metabolism. Our objective was to determine if feeding flaxseed oil (∼50% C18:3n-3 cis), high oleic soybean (∼70% C18:1 cis-9), or milk fat C16:0) alters expression of PC, PCK1, PCK2 the flow carbons from propionate pyruvate into TCA cycle in preruminating calves. Male Holstein calves (n = 40) were assigned a diet skim with either: 3% (MF; n 8), (Flax; (HOSO; 1.5% MF + (MF-HOSO; (MF-Flax; 8) d 14 21 postnatal. At postnatal, liver biopsy taken...
Muscle biopsies of the longissimus dorsi prepartum dairy cattle indicate that molecular signatures support distinct metabolic strategies between low- and high-muscle branched-chain volatile fatty acid supplementation increased substrates for energy generation.
Periparturient dairy cows experience metabolic adaptations to prepare for increased nutrient requirements of the fetus and onset lactation. Adaptations include peripheral tissue insulin resistance, which can be evaluated experimentally using intravenous glucose tolerance tests (IVGTT). The objective this study was determine if prepartum skeletal muscle reserves supplementation branched-chain volatile fatty acids (BCVFA) in period affected blood glucose, β-hydroxybutyrate (BHB),...