A5054399421- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Respiratory viral infections research
- Plant Virus Research Studies
- Environmental DNA in Biodiversity Studies
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Microbial bioremediation and biosurfactants
- Groundwater flow and contamination studies
- Wastewater Treatment and Nitrogen Removal
- Forest Insect Ecology and Management
- Underwater Vehicles and Communication Systems
- Energy Harvesting in Wireless Networks
- Polyomavirus and related diseases
- Vector-borne infectious diseases
- Environmental remediation with nanomaterials
- SARS-CoV-2 detection and testing
- Toxic Organic Pollutants Impact
- Phytoplasmas and Hemiptera pathogens
- Plant and Fungal Interactions Research
University of Delaware
2004-2013
Biotechnology Institute
2005-2011
Michigan State University
2000-2003
Oklahoma State University
2003
University of Michigan
2003
Nagaoka University of Technology
2000
A side-by-side comparison of bioaugmentation, biostimulation, and a recirculation-only control was implemented in chloroethene-contaminated aquifer. The objective to develop contaminant mass balance based on the analysis groundwater aquifer solids quantify key dechlorinating populations during treatment determine their relation rate chloroethenes removed. bioaugmentation strategy, using Dehalococcoides-containing PCE-to-ethene inoculum enriched from same aquifer, resulted near-stoichiometric...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 41:221-232 (2005) - doi:10.3354/ame041221 Tangential flow diafiltration: an improved technique for estimation of virioplankton production Danielle M. Winget1, Kurt E. Williamson2, Rebekah R. Helton2, K. Eric Wommack1,2,* 1College Marine Studies, and 2Department Plant Soil Sciences, Delaware Biotechnology Institute,...
The Chesapeake Bay, a seasonally variable temperate estuary, provides natural laboratory for examining the fluctuations and impacts of viral lysis on aquatic microorganisms. Viral abundance (VA) production (VP) were monitored in Bay over 4 1/2 annual cycles, producing unique, long-term, interannual study virioplankton production. High dynamic VP rates, averaging 7.9 × 10 6 viruses per mL h, indicate that significant fraction microorganisms Chesapeake. Viral-mediated bacterial mortality, VA,...
ABSTRACT Accurate enumeration of viruses within environmental samples is critical for investigations the ecological role and viral infection microbial communities. This report evaluates differences in bacterial direct counts between estuarine sediment which were either immediately processed onboard ship or frozen at −20°C later processed. Viral abundances recorded three stations spanning length Chesapeake Bay April June 2003 fractions: pore water (PW), whole (WS), after removal (AP). No...
ABSTRACT Direct enumeration and genetic analyses indicate that aquatic sediments harbor abundant diverse viral communities. Thus far, synecological analysis of estuarine sediment diversity over an annual cycle has not been reported. This oversight is due in large part to a lack molecular approaches for assessing within collection environmental samples. Here, randomly amplified polymorphic DNA PCR (RAPD-PCR) was used examine genotypic Chesapeake Bay sediments. Using single 10-mer...
The Gram-positive bacterium Rhodococcus sp. strain RHA1, naturally containing the biphenyl pathway, was electroporated with a broad host range plasmid 4-chlorobenzoate (4-CBA) degradation operon (fcb) isolated from Arthrobacter globiformis KZT1. recombinant grew in medium 4-CBA and 4-chlorobiphenyl (4-CB) as only source of carbon, stoichiometric release chloride molar growth yield on 4-CB that suggested utilization both rings. In resting cell assays, similar rates were observed for wild-type...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 41:209-219 (2005) - doi:10.3354/ame041209 Evaluation of incubation-based methods for estimating virioplankton production in estuaries Rebekah R. Helton1, Matthew T. Cottrell2, David L. Kirchman2, K. Eric Wommack1, 2, * 1Department Plant and Soil Sciences, Delaware Biotechnology Institute, University Delaware, 15...
Despite significant implications of viral activity in sediment ecosystems, there are limited data describing how assemblages respond to broader ecosystem changes. To document this, the spatial and temporal dynamics bacterial abundance (BA) changes morphological distribution viruses were examined within three salinity regions over 2 years. Viral abundances (VA) ranged from 0.2 17 × 10(10) mL(-1) while direct counts 3.8 37 10(8) cells sediment. Peaks valleys extracted bacteria surface...
Abstract Virtually every study that has used transmission electron microscopy (TEM) to estimate viral diversity acknowledged loss of phage tails during sample preparation may have biased the results. However, magnitude this potential bias yet be constrained. To characterize biases in virus morphological due tail loss, six strains representing order Caudovirales were inoculated into sterile sediments and soils. Phage particles then extracted using standard methods. Morphologies compared those...
This protocoloutlines and evaluates the filtration steps needed for preparation of samples (viral concentrates) containing a density viral particles concentrated from large volumes natural water samples.(Fig. 2A B)
Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – 30,