A5005909677- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- RNA Research and Splicing
- Cancer-related gene regulation
- HVDC Systems and Fault Protection
- PARP inhibition in cancer therapy
- Cardiac Structural Anomalies and Repair
- Genomics and Phylogenetic Studies
- Genetic factors in colorectal cancer
Genome Institute of Singapore
2018-2024
Agency for Science, Technology and Research
2020-2024
Institute of Molecular and Cell Biology
2018
Abstract Various methyltransferases and demethylases catalyse methylation demethylation of N 6 -methyladenosine (m6A) ,2′-O-dimethyladenosine (m6Am) but precise methylomes uniquely mediated by each methyltransferase/demethylase are still lacking. Here, we develop m6A-Crosslinking-Exonuclease-sequencing (m6ACE-seq) to map transcriptome-wide m6A m6Am at quantitative single-base-resolution. This allows for the generation a comprehensive atlas distinct every individual known methyltransferase or...
Abstract N 6-methylation of 2′-O-methyladenosine (Am) in RNA occurs eukaryotic cells to generate N6,2′-O-dimethyladenosine (m6Am). Identification the methyltransferase responsible for m6Am catalysis has accelerated studies on function processing. While is generally found first transcribed nucleotide mRNAs, modification also internally within U2 snRNA. However, writer required catalyzing internal formation had remained elusive. By sequencing transcriptome-wide methylation at...
N6-methyldeoxyadenosine (6mA) is a well-characterized DNA modification in prokaryotes but reports on its presence and function mammals have been controversial. To address this issue, we established the capacity of 6mA-Crosslinking-Exonuclease-sequencing (6mACE-seq) to detect genome-wide 6mA at single-nucleotide-resolution, demonstrating by accurately mapping synthesized bacterial genomes. Using 6mACE-seq, generated human-genome-wide map that reproduced known enrichment active...
The identification of cancer driver genes from sequencing data has been crucial in deepening our understanding tumor biology and expanding targeted therapy options. However, apart the most commonly altered genes, mechanisms underlying contribution other mutations to acquisition remain understudied. Leveraging on whole-exome largest Asian lung adenocarcinoma (LUAD) cohort (n = 302), we now functionally assess mechanistic role a novel driver, PARP4.
Abstract Differences in RNA expression can provide insights into the molecular identity of a cell, pathways involved human diseases, and variation levels across patients associated with clinical phenotypes. modifications such as m6A have been found to contribute functions RNAs. However, quantification differences has challenging. Here we develop computational method (xPore) identify differential from direct sequencing data. We evaluate our on transcriptome-wide profiling data, demonstrating...
Abstract N 6 -methylation of 2’-O-methyladenosine (Am) in RNA occurs eukaryotic cells to generate ,2’-O-dimethyladenosine (m6Am). Identification the methyltransferase responsible for m6Am catalysis has accelerated studies on function processing. While is generally found first transcribed nucleotide mRNAs, modification also internally within U2 snRNA . However, writer required catalyzing internal formation had remained elusive. By sequencing transcriptome-wide methylation at...