A5018487490- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Xenotransplantation and immune response
- Renal Transplantation Outcomes and Treatments
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
Northwestern University
2023-2025
University of Rochester
1998
Abstract The vast complexity of the proteome currently overwhelms any single analytical technology in capturing full spectrum proteoform diversity. In this study, we evaluated complementarity two cutting-edge proteomic technologies—single-molecule protein sequencing and individual ion mass spectrometry—for analyzing recombinant human IL-6 (rhIL-6) at amino acid, peptide, intact levels. For single-molecule sequencing, employ recently released Platinum ® instrument. Next-Generation Protein...
Solid organ transplant recipients with immunosuppressant regimens to prevent rejection are less able mount effective immune responses pathogenic infection. Here, we apply a recently reported mass spectrometry-based serological approach known as Ig-MS characterize against infection SARS-CoV-2 in cohorts of and immunocompetent controls, both at single early time point following COVID-19 diagnosis well over the course one-month postdiagnosis. We found that antibody repertoires generated by do...
504 Introduction: Intrathymic mechanisms are fundamental to the development of self-tolerance and may be responsible for tolerance seen in mixed chimeras. Transplantation a chimeric thymus would enable study intrathymic events by isolating from effects peripheral alloantigen radiation neonatal However traditional methods nonvascularized transplantation limited as grafts undergo ischemia necrosis exhibit destruction thymic architecture. To circumvent these drawbacks, we developed method adult...