A5017745898- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Polyamine Metabolism and Applications
- Advanced biosensing and bioanalysis techniques
- Cannabis and Cannabinoid Research
- RNA Research and Splicing
- Insect and Arachnid Ecology and Behavior
- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Pancreatic function and diabetes
- RNA and protein synthesis mechanisms
- Physiological and biochemical adaptations
- Circadian rhythm and melatonin
- Neuroscience of respiration and sleep
Imperial College London
2021-2024
University of Cambridge
2019-2021
Sapienza University of Rome
2021
Cancer Research UK
2019
Cancer Research UK Cambridge Center
2019
The PIWI-interacting RNA (piRNA) pathway is a small RNA-based immune system that controls the expression of transposons and maintains genome integrity in animal gonads. In Drosophila, piRNA-guided silencing achieved, part, via co-transcriptional repression by Piwi. This depends on Panoramix (Panx); however, precisely how an binding event silences transcription remains to be determined. Here we show Nuclear Export Factor 2 (Nxf2) its co-factor, Nxt1, form complex with Panx are required for...
Abstract Living organisms synchronize their biological activities with the earth’s rotation through circadian clock, a molecular mechanism that regulates biology and behavior daily. This synchronization factually maximizes positive (e.g., social interactions, feeding) during safe periods, minimizes exposure to dangers predation, darkness) typically at night. Beyond basic regulation, some behaviors like sleep have an additional layer of homeostatic control, ensuring those essential are...
The Piwi-interacting RNA (piRNA) pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires specialized machinery converts long single-stranded precursors into RNAs of ∼25-nucleotides length. This process involves factors operate two different subcellular compartments: the nuage/Yb body and mitochondria. How these sites communicate to achieve accurate substrate selection efficient processing remains unclear. Here, we...
The PIWI-interacting RNA (piRNA) pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. In Drosophila , piRNAs are intergenerationally inherited through the maternal lineage, and this has demonstrated importance specification of piRNA source loci silencing I- P- elements cells daughters. Maternally Piwi protein enters somatic nuclei early embryos prior to zygotic activation persists therein for roughly half time required complete...
Abstract Most living organisms have evolved to synchronize their biological activities with the earth’s rotation, a daily regulation of biology and behaviour controlled by an evolutionary conserved molecular machinery known as circadian clock. For most animals, mechanisms are meant maximize exposure positive ( e.g.: social interactions, mating, feeding – generally during day) minimize peril predation, weather, darkness night 1 ). On top regulation, some behaviours also feature second layer...
Microsatellite expansions of CCTG repeats in the cellular nucleic acid-binding protein (CNBP) gene leads to accumulation toxic RNA and have been associated with myotonic dystrophy type 2 (DM2). However, it is still unclear whether dystrophic phenotype also linked CNBP decrease, a conserved CCHC-type zinc finger RNA-binding that regulates translation required for mammalian development. Here, we show depletion Drosophila muscles causes ageing-dependent locomotor defects are correlated impaired...
ABSTRACT The piRNA pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. biogenesis requires specialised machinery converts long single-stranded precursors into RNAs of ~25-nucleotides length. This process involves factors operate two different subcellular compartments: the nuage/Yb-body and mitochondria. How these sites communicate to achieve accurate substrate selection efficient processing remains unclear. Here, we investigate previously...
Summary The PIWI-interacting RNA (piRNA) pathway is a small RNA-based immune system that controls the expression of transposons and maintains genome integrity in animal gonads. In Drosophila , piRNA-guided silencing achieved, part, via co-transcriptional repression by Piwi. This depends on Panoramix (Panx); however, precisely how an binding event silences transcription remains to be determined. Here we show Nuclear Export Factor 2 (Nxf2) its cofactor, Nxt1, form complex with Panx are...
ABSTRACT Microsatellite expansions of CCTG repeats in the CNBP gene leads to accumulation toxic RNA and have been associated DM2. However, it is still unclear whether dystrophic phenotype also linked decrease, a conserved CCHC-type zinc finger binding protein that regulates translation required for mammalian development. Here we show depletion Drosophila muscles causes age-dependent locomotor defects are correlated with impaired polyamine metabolism. We demonstrate levels ornithine...
Abstract The PIWI-interacting RNA (piRNA) pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. In Drosophila , piRNAs are intergenerationally inherited through the maternal lineage, and this has demonstrated importance specification of piRNA source loci silencing I- P- elements cells daughters. Maternally Piwi protein enters somatic nuclei early embryos prior to zygotic activation persists therein for roughly half time required...