A5012256403- Sulfur Compounds in Biology
- Eicosanoids and Hypertension Pharmacology
- Nitric Oxide and Endothelin Effects
- Renin-Angiotensin System Studies
- Immune Cell Function and Interaction
- Genomics, phytochemicals, and oxidative stress
- Fibroblast Growth Factor Research
- Estrogen and related hormone effects
- Pharmacogenetics and Drug Metabolism
- Advanced Glycation End Products research
- Chronic Lymphocytic Leukemia Research
- Immune Response and Inflammation
- Free Radicals and Antioxidants
- Pharmacological Effects of Natural Compounds
- Neuroscience of respiration and sleep
- Bioactive Compounds and Antitumor Agents
- T-cell and B-cell Immunology
- Metabolomics and Mass Spectrometry Studies
- Galectins and Cancer Biology
- Phytochemicals and Antioxidant Activities
- Cardiac Ischemia and Reperfusion
- COVID-19 Clinical Research Studies
- Effects and risks of endocrine disrupting chemicals
- Chemical synthesis and alkaloids
- Bone health and treatments
University of Basilicata
2020-2024
Diabetes is associated with severe vascular complications involving the impairment of endothelial nitric oxide synthase (eNOS) as well cystathionine γ-lyase (CSE) activity. eNOS function suppressed in hyperglycaemic conditions, resulting reduced NO bioavailability, which paralleled by levels hydrogen sulfide (H2S). Here we have addressed molecular basis interplay between and CSE pathways. We tested impact H2S replacement using mitochondrial-targeted donor AP123 isolated vessels cultured...
Here, by using in vitro and ex vivo approaches, we elucidate the impairment of hydrogen sulfide (H2S) pathway vascular complications associated with metabolic syndrome (MetS). In model simulating hyperlipidemic/hyperglycemic conditions, observe significant hallmarks endothelial dysfunction, including eNOS/NO signaling impairment, ROS overproduction, a reduction CSE-derived H2S. Transitioning to an db/db mice, genetic MetS model, identify downregulation CBS CSE expression aorta, coupled...
Preservation of vascular wall integrity against degenerative processes associated with ageing, fat-rich diet and metabolic diseases is a timely therapeutical challenge. The loss endothelial function leads to cardiovascular multiorgan inflammation. protective effects the H2S-donor erucin, an isothiocyanate purified by Eruca sativa Mill. seeds, were evaluated on human smooth muscle cells. In particular, erucin actions cell viability, ROS, caspase 3/7, inflammatory markers levels...
Vascular inflammation (VI) represents a pathological condition that progressively affects the integrity and functionality of vascular wall, thus leading to endothelial dysfunction onset several cardiovascular diseases. Therefore, research novel compounds able prevent VI compelling need. In this study, we tested erucin, natural isothiocyanate H2S-donor derived from Eruca sativa Mill. (Brassicaceae), in an vivo mouse model lipopolysaccharide (LPS)-induced peritonitis, where it significantly...
Hydrogen sulfide (H2S) is a gasotransmitter deeply involved in cardiovascular homeostasis and implicated the myocardial protection against ischemia/reperfusion. The post-translational persulfidation of cysteine residues has been identified as mechanism through which H2S regulates plethora biological targets. Erucin (ERU) an isothiocyanate produced upon hydrolysis glucosinolate glucoerucin, presents edible plants Brassicaceae family, such Eruca sativa Mill., it emerged slow long-lasting...
Modulation of mitochondrial K channels represents a pharmacological strategy to promote cardioprotective effects. Isothiocyanates emerge as molecules capable releasing hydrogen sulfide (H2S), an endogenous pleiotropic gasotransmitter responsible for anti-ischemic effects also through the involvement mitoK channels. Erucin (ERU) is natural isothiocyanate resulting from enzymatic hydrolysis glucosinolates (GSLs) present in Eruca sativa Mill. seeds, edible plant Brassicaceae family. In this...
Phenolic compounds of red wine powder (RWP) extracted from the Italian Aglianico del Vulture have been investigated for potential immunomodulatory and anti‐inflammatory capacity on human macrophages. These reduce secretion IL‐1 β , IL‐6, TNF‐ α proinflammatory cytokines increase release IL‐10 cytokine induced by lipopolysaccharide (LPS). In addition, RWP restores Annexin A1 levels, thus involving activation proresolutive pathways. Noteworthy, lowers NF‐ κ B protein promoter activity, nuclear...
The interplay between immune activation and regulation is a fundamental aspect of the functional harmony system. This delicate balance essential to triggering correct effective responses against pathogens while preventing excessive inflammation immunopathogenic mechanisms autoimmunity. knowledge all involved in not yet definitive, and, probably, overall picture much broader than what has been described scientific literature so far. Given plasticity system diversity organisms, it highly...
Chronic lymphocytic leukaemia (CLL) is characterised by the expansion of a neoplastic mature B cell clone. CLL clinical outcome very heterogeneous, with some subjects never requiring therapy and showing an aggressive disease. Genetic epigenetic alterations pro-inflammatory microenvironment influence progression prognosis. The involvement immune-mediated mechanisms in control needs to be investigated. We analyse activation profile innate adaptive cytotoxic immune effectors cohort 26 patients...
Clodronate is a bisphosphonate agent commonly used as anti-osteoporotic drug. Throughout its use, additional anti-inflammatory and analgesic properties have been reported, although the benefits described in literature could not solely relate to their inhibition of bone resorption. Thus, purpose our vitro study investigate whether there are underlying mechanisms explaining effect clodronate possibly involving hydrogen sulphide (H2S). Immortalised fibroblast-like synoviocyte cells (K4IM) were...